#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5251097	B	Inhibition of human full length USP21 using Btn-Ahx-PNIRFLD-K(Ubi)-LPQQT-GD-amide as substrate preincubated for 15 to 20 mins followed by substrate addition and measured after 25 mins by HTRF assay	Homo sapiens	22	ALA5247643	single protein format	Scientific Literature
2.	ALA5251098	B	Inhibition of human USP21 using ubiquitin rhodamine 110 110 as substrate preincubated for 15 to 20 mins followed by enzyme addition and measured after 25 mins by fluorometric assay	Homo sapiens	22	ALA5247643	single protein format	Scientific Literature
3.	ALA5251115	P	Lipophilicity, logD of compound at pH 7.5 by HPCL analysis		22	ALA5247643	small-molecule physicochemical format	Scientific Literature
4.	ALA5251116	P	Aqueous solubility of compound in PBS at pH 6.5 incubated for 24 hrs by HPLC-MS/MS analysis		12	ALA5247643	small-molecule physicochemical format	Scientific Literature
5.	ALA5251117	B	Inhibition of human full length USP21 using Btn-Ahx-PNIRFLD-K(Ubi)-LPQQT-GD-amide as substrate assessed as maximal response preincubated for 15 to 20 mins followed by substrate addition and measured after 25 mins by HTRF assay	Homo sapiens	11	ALA5247643	single protein format	Scientific Literature
6.	ALA5251118	B	Inhibition of human USP21 using ubiquitin rhodamine 110 as substrate assessed as maximal response preincubated for 15 to 20 mins followed by enzyme addition and measured after 25 mins by fluorometric assay	Homo sapiens	8	ALA5247643	single protein format	Scientific Literature
7.	ALA5251119	B	Inhibition of full-length human USP21 transfected in human HEK293T cells co-transfected with NF-kappaB reporter firefly luciferase plasmid assessed as NF-kappaB activation at 10 uM and measured after 20 hrs by dual-luciferase reporter gene assay	Homo sapiens	4	ALA5247643	cell-based format	Scientific Literature
8.	ALA5251120	B	Binding affinity to C-terminally NanoLuc HiBiT-tagged USP21 transfected in human HEK293T cells assessed as increase in thermal stabilization at 49 degreeC at 50 to 100 uM incubated for 1 hrs by Nano-Glo substrate based CESTA analysis relative to control	Homo sapiens	5	ALA5247643	cell-based format	Scientific Literature
9.	ALA5251121	A	Apparent permeability across apical to basolateral side in human Caco-2 cells at 2 uM and measured for 2 hrs by LC-MS/MS analysis	Homo sapiens	6	ALA5247643	cell-based format	Scientific Literature
10.	ALA5251122	A	Efflux ratio of apparent permeability across basolateral to apical side over apical to basolateral side in human Caco-2 cells at 2 uM and measured for 2 hrs by LC-MS/MS analysis	Homo sapiens	6	ALA5247643	cell-based format	Scientific Literature