# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5252340 | B | Binding affinity to 5'-Cy5-labelled SARS-CoV-2 frameshift element RNA at 10 uM by microarray-based fluorescence analysis | Severe acute respiratory syndrome coronavirus 2 | 1 | assay format | Scientific Literature | ||
2. | ALA5252341 | B | Binding affinity to SARS-CoV-2 frameshift element RNA at 150 uM by water ligand observed gradient spectroscopic analysis | Severe acute respiratory syndrome coronavirus 2 | 3 | assay format | Scientific Literature | ||
3. | ALA5252342 | B | Binding affinity to 5'-biotin-labelled SARS-CoV-2 frameshift element RNA assessed as dissociation constant by SPR analysis | Severe acute respiratory syndrome coronavirus 2 | 3 | assay format | Scientific Literature | ||
4. | ALA5252343 | B | Binding affinity to 5'-CY5-labelled SARS-CoV-2 frameshift element RNA assessed as dissociation constant by MST analysis | Severe acute respiratory syndrome coronavirus 2 | 2 | assay format | Scientific Literature | ||
5. | ALA5252345 | B | Binding affinity to SARS-CoV-2 frameshift element RNA assessed as thermal stability by measuring change in melting temperature of RNA at 150 uM by circular dichroism analysis | Severe acute respiratory syndrome coronavirus 2 | 1 | assay format | Scientific Literature | ||
6. | ALA5252346 | B | Binding affinity to SARS-CoV-2 frameshift element RNA assessed as thermal stability by measuring change in melting temperature of RNA at 500 uM by circular dichroism analysis | Severe acute respiratory syndrome coronavirus 2 | 1 | assay format | Scientific Literature | ||
7. | ALA5252348 | B | Binding affinity to 5'-CY5-labelled SARS-CoV-2 frameshift element RNA by MST analysis | Severe acute respiratory syndrome coronavirus 2 | 27 | assay format | Scientific Literature | ||
8. | ALA5252349 | B | Binding affinity to 5'-CY5-labelled SARS-CoV-2 frameshift element RNA by SPR analysis | Severe acute respiratory syndrome coronavirus 2 | 25 | assay format | Scientific Literature | ||
9. | ALA5252351 | T | Cytotoxicity against human MCF7 cells assessed as cell viability at 50 uM measured after 48 hrs by Cell-titer Glo luminescent assay relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
10. | ALA5252352 | B | Binding affinity to wild-type SARS-CoV-2 frameshift element RNA pseudoknot sequence transfected with Renilla/Firefly luciferase in rabbit reticulocyte lysate assessed as frameshifting by measuring ratio of renilla/firefly luminescence by dual-luciferase reporter assay | Severe acute respiratory syndrome coronavirus 2 | 4 | subcellular format | Scientific Literature | ||
11. | ALA5252353 | B | Binding affinity to wild-type SARS-CoV-2 frameshift element RNA pseudoknot sequence transfected with Renilla/Firefly luciferase in rabbit reticulocyte lysate assessed as translation by measuring ratio of renilla luminescence by dual-luciferase reporter assay | Severe acute respiratory syndrome coronavirus 2 | 4 | subcellular format | Scientific Literature | ||
12. | ALA5252354 | B | Binding affinity to wild-type Rous sarcoma virus frameshift element RNA pseudoknot sequence transfected with Renilla/Firefly luciferase in rabbit reticulocyte lysate assessed as frameshifting by measuring ratio of renilla/firefly luminescence by dual-luciferase reporter assay | Rous sarcoma virus | 4 | subcellular format | Scientific Literature | ||
13. | ALA5252355 | B | Binding affinity to wild-type Rous sarcoma virus frameshift element RNA pseudoknot sequence transfected with Renilla/Firefly luciferase in rabbit reticulocyte lysate assessed as translation by measuring ratio of renilla luminescence by dual-luciferase reporter assay | Rous sarcoma virus | 4 | subcellular format | Scientific Literature | ||
14. | ALA5252359 | F | Inhibition of programmed -1 ribosomal frameshifting in human MCF7 cells harboring pCherryGFP-CoV2-WT plasmid assessed as inhibition of GFP expression incubated for 30 mins by fluorescence microscopic analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
15. | ALA5252361 | F | Inhibition of programmed -1 ribosomal frameshifting in human MCF7 cells harboring pCherryGFP-Inframe-noFSE plasmid assessed as inhibition of GFP expression incubated for 30 mins by fluorescence microscopic analysis | Homo sapiens | 1 | cell-based format | Scientific Literature |