| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5250787 | P | Thermodynamic aqueous solubility of compound at 25 degreeC measured after 24 hrs by HPLC analysis | 8 | small-molecule physicochemical format | Scientific Literature | |||
| 2. | ALA5250788 | B | Inhibition of HIS-tagged recombinant Leishmania infantum TryR oxidoreductase activity expressed in Escherichia coli in the presence of NADPH by spectrophotometry based DTNB-coupled assay | Leishmania infantum | 17 | assay format | Scientific Literature | ||
| 3. | ALA5250789 | B | Disruption of HIS-FLAG-tagged recombinant Leishmania infantum TryR dimerization expressed in Escherichia coli Rossetta BL21 (DE3) cells incubated for 16 hrs by ELISA analysis | Leishmania infantum | 17 | cell-based format | Scientific Literature | ||
| 4. | ALA5250791 | B | Inhibition of HIS-tagged recombinant Trypanosoma brucei TryR oxidoreductase activity expressed in Escherichia coli in the presence of NADPH by spectrophotometry based DTNB-coupled assay | Trypanosoma brucei | 3 | assay format | Scientific Literature | ||
| 5. | ALA5250792 | B | Inhibition of HIS-tagged recombinant Trypanosoma cruzi TryR oxidoreductase activity expressed in Escherichia coli in the presence of NADPH by spectrophotometry based DTNB-coupled assay | Trypanosoma cruzi | 3 | assay format | Scientific Literature | ||
| 6. | ALA5250793 | B | Inhibition of HIS-tagged recombinant Trypanosoma congolense TryR oxidoreductase activity expressed in Escherichia coli in the presence of NADPH by spectrophotometry based DTNB-coupled assay | Trypanosoma congolense | 3 | assay format | Scientific Literature | ||
| 7. | ALA5250794 | B | Inhibition of HIS-tagged recombinant human GR oxidoreductase activity expressed in Escherichia coli in the presence of NADPH by spectrophotometry based DTNB-coupled assay | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 8. | ALA5250797 | F | Antileishmanial activity against Leishmania infantum MCAN/ES/89/IPZ229/1/89 axenic amastigotes assessed as reduction in parasite viability treated for 24 hrs by PI staining based flow cytometry | Leishmania infantum | 17 | organism-based format | Scientific Literature | ||
| 9. | ALA5250798 | T | Cytotoxicity against human HepG2 cells assessed as reduction in cell proliferation treated for 24 hrs by crystal violet staining based analysis | Homo sapiens | 17 | cell-based format | Scientific Literature | ||
| 10. | ALA5250799 | T | Selectivity index, ratio of CC50 for human HepG2 cells to EC50 for Leishmania infantum MCAN/ES/89/IPZ229/1/89 axenic amastigotes | 18 | assay format | Scientific Literature | |||
| 11. | ALA5250800 | F | Antileishmanial activity against GFP-expressing Leishmania infantum intracellular amastigotes infected in human THP-1 derived macrophage cells assessed as reduction in parasite viability measured for 72 hrs by resazurin/alamar blue fluorometric assay | Leishmania infantum | 14 | organism-based format | Scientific Literature | ||
| 12. | ALA5250801 | T | Cytotoxicity against human HepG2 cells assessed as reduction in cell proliferation measured after 72 hrs by crystal violet staining based analysis | Homo sapiens | 14 | cell-based format | Scientific Literature | ||
| 13. | ALA5250802 | T | Selectivity index, ratio of CC50 for human HepG2 cells to EC50 for GFP-expressing Leishmania infantum intracellular amastigotes infected in human THP-1 derived macrophage cells | 14 | cell-based format | Scientific Literature | |||
| 14. | ALA5250803 | F | Antileishmanial activity against Leishmania infantum promastigotes assessed as reduction in non-protein thiol content at 75 uM incubated for 1 hrs by CMF diacetate probe based flow cytometry relative to control | Leishmania infantum | 1 | organism-based format | Scientific Literature | ||
| 15. | ALA5250804 | F | Antileishmanial activity against Leishmania infantum promastigotes assessed as reduction in total thiol content at 75 uM treated for 1 hr by CMF diacetate probe based flow cytometry relative to control | Leishmania infantum | 1 | organism-based format | Scientific Literature | ||
| 16. | ALA5250807 | F | Antileishmanial activity against H2O2-induced Leishmania infantum promastigotes assessed as reduction in non-protein thiol content at 75 uM treated for 1 hr in presence of BSO by CMF diacetate probe based flow cytometry relative to control | Leishmania infantum | 1 | organism-based format | Scientific Literature | ||
| 17. | ALA5250808 | B | Mixed non-competitive inhibition of Leishmania infantum TryR using TS2 as substrate assessed as increase in apparent inhibition constant of initial EI complex by plot analysis | Leishmania infantum | 1 | single protein format | Scientific Literature | ||
| 18. | ALA5250809 | B | Selectivity ratio of IC50 for inhibition of HIS-tagged recombinant Trypanosoma brucei TryR oxidoreductase activity expressed in Escherichia coli to IC50 for inhibition of HIS-tagged recombinant Leishmania infantum TryR oxidoreductase activity expressed in Escherichia coli | 2 | assay format | Scientific Literature | |||
| 19. | ALA5250810 | B | Selectivity ratio of IC50 for inhibition of HIS-tagged recombinant Trypanosoma cruzi TryR oxidoreductase activity expressed in Escherichia coli to IC50 for inhibition of HIS-tagged recombinant Leishmania infantum TryR oxidoreductase activity expressed in Escherichia coli | 2 | assay format | Scientific Literature | |||
| 20. | ALA5250811 | B | Time-dependent inhibition of Leishmania infantum TryR using TS2 as substrate assessed as decrease in initial velocity rate at fixed TS2 concentration | Leishmania infantum | 1 | single protein format | Scientific Literature |
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