#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5239507	F	Antiproliferative activity against human K562 cells measured after 72 hrs by MTT assay	Homo sapiens	41	ALA5236615	cell-based format	Scientific Literature
2.	ALA5239508	F	Antiproliferative activity against human MCF7 cells measured after 72 hrs by MTT assay	Homo sapiens	41	ALA5236615	cell-based format	Scientific Literature
3.	ALA5239509	F	Antiproliferative activity against human HT-29 cells measured after 72 hrs by MTT assay	Homo sapiens	41	ALA5236615	cell-based format	Scientific Literature
4.	ALA5239510	F	Antiproliferative activity against human HCT-116 cells measured after 72 hrs by MTT assay	Homo sapiens	41	ALA5236615	cell-based format	Scientific Literature
5.	ALA5239511	A	Metabolic stability in human liver microsomes assessed as half life at 1 uM by LC/MS analysis	Homo sapiens	8	ALA5236615	microsome format	Scientific Literature
6.	ALA5239512	A	Metabolic stability in human liver microsomes assessed as intrinsic clearance at 1 uM by LC/MS analysis	Homo sapiens	8	ALA5236615	microsome format	Scientific Literature
7.	ALA5239513	B	Inhibition of porcine brain tubulin assessed as changes in the fluorescence intensity measured for 60 mins by cell free tubulin polymerization assay	Sus scrofa	3	ALA5236615	assay format	Scientific Literature
8.	ALA5239514	B	Inhibition of [3H]colchicine binding to tubulin (unknown origin) at 1 uM by competition scintillation proximity assay relative to control	Homo sapiens	3	ALA5236615	protein complex format	Scientific Literature
9.	ALA5239515	B	Inhibition of [3H]colchicine binding to tubulin (unknown origin) at 5 uM by competition scintillation proximity assay relative to control	Homo sapiens	3	ALA5236615	protein complex format	Scientific Literature
10.	ALA5239516	P	Aqueous solubility in pH 7.4 PBS buffer by HPLC-UV analysis		2	ALA5236615	small-molecule physicochemical format	Scientific Literature
11.	ALA5239517	B	Binding affinity to porcine brain tubulin assessed as changes in the fluorescence incubated for 10 mins tryptophan-based binding assay	Sus scrofa	3	ALA5236615	assay format	Scientific Literature
12.	ALA5239518	B	Binding affinity to beta-tubulin colchicine binding site in human MCF-7 cells assessed as inhibition of EBI-beta-tubulin adduct band formation preincubated for 2 hrs followed by EBI addition and measured after 1.5 hrs by EBI competition based Western blotting analysis	Homo sapiens	2	ALA5236615	cell-based format	Scientific Literature
13.	ALA5239519	B	Disruption of microtubule network in human MCF7 cells assessed as broken microtubule filaments at 20 nM measured after 6 hrs by immunofluorescence assay	Homo sapiens	2	ALA5236615	cell-based format	Scientific Literature
14.	ALA5239520	F	Induction of cell cycle arrest in human MCF7 cells assessed as accumulation of cells at G2/M phase at 5 nM measured after 24 hrs by flow cytometry relative to control	Homo sapiens	2	ALA5236615	cell-based format	Scientific Literature
15.	ALA5239521	F	Induction of cell cycle arrest in human MCF7 cells assessed as accumulation of cells at G2/M phase at 10 nM measured after 24 hrs by flow cytometry relative to control	Homo sapiens	2	ALA5236615	cell-based format	Scientific Literature
16.	ALA5239522	F	Induction of cell cycle arrest in human MCF7 cells assessed as accumulation of cells at G2/M phase at 20 nM measured after 24 hrs by flow cytometry relative to control	Homo sapiens	2	ALA5236615	cell-based format	Scientific Literature
17.	ALA5239523	F	Induction of cell cycle arrest in human MCF7 cells assessed as cdc25c activation at 5 to 25 nM incubated for 24 hrs by Western blotting analysis	Homo sapiens	2	ALA5236615	cell-based format	Scientific Literature
18.	ALA5239524	F	Induction of cell cycle arrest in human MCF7 cells assessed as arrest in G2/M phase by measuring accumulation of cyclin B1 at 5 to 25 nM incubated for 24 hrs by Western blotting analysis	Homo sapiens	2	ALA5236615	cell-based format	Scientific Literature
19.	ALA5239525	F	Induction of cell cycle arrest in human MCF7 cells assessed as arrest in G2/M phase by measuring accumulation of CDK7 at 5 to 25 nM incubated for 24 hrs by Western blotting analysis	Homo sapiens	2	ALA5236615	cell-based format	Scientific Literature
20.	ALA5239526	F	Induction of cell cycle arrest in human MCF7 cells assessed as arrest in G2/M phase by measuring accumulation of p21 at 5 to 25 nM incubated for 24 hrs by Western blotting analysis	Homo sapiens	2	ALA5236615	cell-based format	Scientific Literature