| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5251097 | B | Inhibition of human full length USP21 using Btn-Ahx-PNIRFLD-K(Ubi)-LPQQT-GD-amide as substrate preincubated for 15 to 20 mins followed by substrate addition and measured after 25 mins by HTRF assay | Homo sapiens | 22 | single protein format | Scientific Literature | ||
| 2. | ALA5251098 | B | Inhibition of human USP21 using ubiquitin rhodamine 110 110 as substrate preincubated for 15 to 20 mins followed by enzyme addition and measured after 25 mins by fluorometric assay | Homo sapiens | 22 | single protein format | Scientific Literature | ||
| 3. | ALA5251099 | B | Inhibition of human USP21 using ubiquitin aminoluciferin as substrate incubated for 25 mins by luminescence based assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 4. | ALA5251100 | B | Binding affinity to recombinant human USP21 assessed as change in melting temperature at 100 uM by thermal shift assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 5. | ALA5251101 | B | Binding affinity to recombinant human N-terminal His6-tagged USP21 (209 to 563 residues) expressed in Escherichia coli BL21 (DE3) assessed as dissociation constant by SPR analysis | Homo sapiens | 3 | assay format | Scientific Literature | ||
| 6. | ALA5251102 | B | Binding affinity to human USP2 assessed as change in melting temperature at 100 uM by thermal shift assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 7. | ALA5251103 | B | Binding affinity to human USP7 assessed as change in melting temperature at 100 uM by thermal shift assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 8. | ALA5251104 | B | Inhibition of human USP21 (209 to 563 residues) expressed in Escherichia coli BL21 (DE3) using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 9. | ALA5251105 | B | Inhibition of human USP2 (251 to 605 residues) expressed in Escherichia coli BL21 (DE3) using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 10. | ALA5251106 | B | Inhibition of human USP4 (1 to 932 residues) expressed in Sf9 cells using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA5251107 | B | Inhibition of human USP30 (57 to 517 residues) expressed in Sf9 cells using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA5251108 | B | Inhibition of human USP7 (1 to 1102 residues) expressed in Sf9 cells using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA5251109 | B | Inhibition of human USP8 (734 to 1110 residues) expressed in Escherichia coli BL21 (DE3) using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 14. | ALA5251110 | B | Inhibition of human USP15 (1 to 952 residues) expressed in Sf9 cells using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA5251111 | B | Inhibition of human USP22 (1 to 525 residues) expressed in Sf9 cells using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 16. | ALA5251112 | B | Inhibition of human USP9X (1551 to 1970 residues) expressed in Escherichia coli BL21 (DE3) using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 17. | ALA5251113 | B | Inhibition of human USP5 (1 to 835 residues) expressed in Sf9 cells using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA5251114 | B | Inhibition of human Ataxin-3 (1 to 370 residues) expressed in Escherichia coli BL21 (DE3) using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 19. | ALA5251115 | P | Lipophilicity, logD of compound at pH 7.5 by HPCL analysis | 22 | small-molecule physicochemical format | Scientific Literature | |||
| 20. | ALA5251211 | B | Inhibition of human His-tagged USP2 (259 to 605 residues) using ubiquitin rhodamine 110 110 as substrate preincubated for 15 to 20 mins followed by enzyme addition and measured after 25 mins by fluorometric assay | Homo sapiens | 11 | single protein format | Scientific Literature |
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