# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5243014 | B | Inhibition of recombinant full length His-tagged PIP4K2A (unknown origin) expressed in sf21 cells assessed as reduction in ADP production preincubated for 20 mins followed by substrate addition and measured after 60 mins using Dic8-PI5P as substrate in presence of 10 uM ATP by ADP-Glo luminescent assay | Homo sapiens | 56 | ALA5241108 | cell-based format | Scientific Literature | |
2. | ALA5243015 | B | Inhibition of recombinant full length His-tagged PIP4K2A (unknown origin) expressed in sf21 assessed as reduction in PI4,5P2 production preincubated for 20 mins followed by substrate addition and measured after 60 mins using Dic8-PI5P as substrate in presence of 10 uM ATP by HTRF assay | Homo sapiens | 56 | ALA5241108 | cell-based format | Scientific Literature | |
3. | ALA5243016 | B | Inhibition of recombinant full length His-tagged PIP4K2A (unknown origin) expressed in sf21 cells assessed as reduction in ADP production preincubated for 20 mins followed by substrate addition and measured after 60 mins using Dic8-PI5P as substrate in presence of 250 uM ATP by ADP-Glo luminescent assay | Homo sapiens | 56 | ALA5241108 | cell-based format | Scientific Literature | |
4. | ALA5243017 | B | Inhibition of recombinant full length His-tagged PIP4K2A (unknown origin) expressed in sf21 assessed as reduction in PI4,5P2 production preincubated for 20 mins followed by substrate addition and measured after 60 mins using Dic8-PI5P as substrate in presence of 2 mM ATP by HTRF assay | Homo sapiens | 56 | ALA5241108 | cell-based format | Scientific Literature | |
5. | ALA5243018 | B | Inhibition of human PIP4K2A in human THP-1 cells assessed as increase in Akt phosphorylation at Ser473 residue incubated for 2 hrs by HTRF assay | Homo sapiens | 56 | ALA5241108 | cell-based format | Scientific Literature | |
6. | ALA5243020 | P | Lipophilicity, logD of compound at pH 7.5 by reversed-phase HPLC analysis | 52 | ALA5241108 | small-molecule physicochemical format | Scientific Literature | ||
7. | ALA5243021 | A | Apparent permeability across apical to basolateral side in human Caco-2 cells at 2 uM measured after 2 hrs by LC/MS-MS analysis | Homo sapiens | 21 | ALA5241108 | cell-based format | Scientific Literature | |
8. | ALA5243022 | A | Apparent permeability across basolateral to apical side in human Caco-2 cells at 2 uM measured after 2 hrs by LC/MS-MS analysis | Homo sapiens | 3 | ALA5241108 | cell-based format | Scientific Literature | |
9. | ALA5243024 | A | Metabolic stability in Han Wistar rat liver microsomes assessed as intrinsic clearance at 1 uM in presence of NADP and measured upto 60 mins by LC-MS/MS analysis | Rattus norvegicus | 3 | ALA5241108 | microsome format | Scientific Literature | |
10. | ALA5243025 | A | Metabolic stability in Han Wistar rat hepatocytes assessed as intrinsic clearance at 1 uM in presence of NADP and measured upto 90 mins by LC-MS/MS analysis | Rattus norvegicus | 3 | ALA5241108 | cell-based format | Scientific Literature | |
11. | ALA5243026 | A | Metabolic stability in Han Wistar rat liver microsomes assessed as maximum bioavailability at 1 uM in presence of NADP and measured upto 60 mins by LC-MS/MS analysis | Rattus norvegicus | 3 | ALA5241108 | organism-based format | Scientific Literature | |
12. | ALA5243027 | A | Metabolic stability in Han Wistar rat hepatocytes assessed as maximum bioavailability at 1 uM in presence of NADP and measured upto 90 mins by LC-MS/MS analysis | Rattus norvegicus | 3 | ALA5241108 | organism-based format | Scientific Literature | |
13. | ALA5243028 | B | Inhibition of recombinant full length His-tagged PIP4K2A (unknown origin) expressed in sf21 cells assessed as reduction in ADP production preincubated for 20 mins followed by substrate addition and measured after 60 mins using Dic8-PI5P as substrate in presence of 20 uM ATP by ADP-Glo luminescent assay | Homo sapiens | 1 | ALA5241108 | cell-based format | Scientific Literature | |
14. | ALA5243029 | P | Aqueous solubility of compound at pH 6.5 and measured after 24 hrs by HPLC-MS/MS analysis | 3 | ALA5241108 | small-molecule physicochemical format | Scientific Literature |