#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5223630	B	Binding affinity to human wild-type double stranded DNA assessed as thermal stability of compound-DNA complex by measuring change in melting temperature at stoichiometry ratio 1:0.5 by UV melting assay	Homo sapiens	3	ALA5223080	nucleic acid format	Scientific Literature
2.	ALA5223631	B	Binding affinity to human wild-type double stranded DNA assessed as thermal stability of compound-DNA complex by measuring change in melting temperature at stoichiometry ratio 1:1 by UV melting assay	Homo sapiens	3	ALA5223080	nucleic acid format	Scientific Literature
3.	ALA5223632	B	Binding affinity to human wild-type double stranded DNA assessed as thermal stability of compound-DNA complex by measuring change in melting temperature at stoichiometry ratio 1:4 by UV melting assay	Homo sapiens	3	ALA5223080	nucleic acid format	Scientific Literature
4.	ALA5223633	B	Binding affinity to human mutant double stranded DNA assessed as thermal stability of compound-DNA complex by measuring melting temperature change in at stoichiometry ratio 1:0.5 by UV melting assay	Homo sapiens	2	ALA5223080	nucleic acid format	Scientific Literature
5.	ALA5223634	B	Binding affinity to human mutant double stranded DNA assessed as thermal stability of compound-DNA complex by measuring change in melting temperature at stoichiometry ratio 1:1 by UV melting assay	Homo sapiens	2	ALA5223080	nucleic acid format	Scientific Literature
6.	ALA5223635	B	Binding affinity to human mutant double stranded DNA assessed as thermal stability of compound-DNA complex by measuring melting change in temperature at stoichiometry ratio 1:4 by UV melting assay	Homo sapiens	2	ALA5223080	nucleic acid format	Scientific Literature
7.	ALA5223636	B	Binding affinity to human 5'-biotin-double stranded DNA assessed as association rate constant by surface plasmon resonance	Homo sapiens	2	ALA5223080	nucleic acid format	Scientific Literature
8.	ALA5223637	B	Binding affinity to human 5'-biotin-double stranded DNA assessed as dissociation rate constant by surface plasmon resonance	Homo sapiens	2	ALA5223080	nucleic acid format	Scientific Literature
9.	ALA5223638	B	Binding affinity to human 5'-biotin-double stranded DNA assessed as dissociation equilibrium constant by surface plasmon resonance	Homo sapiens	2	ALA5223080	nucleic acid format	Scientific Literature
10.	ALA5223639	B	Binding affinity to wild-type double stranded DNA (unknown origin) by assessed as decrease in compound-DNA complex at >=8 uM by competitive electrophoretic mobility shift assay	Not specified	2	ALA5223080	nucleic acid format	Scientific Literature
11.	ALA5223640	B	Binding affinity to human single stranded DNA (unknown origin) by electrophoretic mobility shift assay	Not specified	3	ALA5223080	assay format	Scientific Literature
12.	ALA5223642	B	Binding affinity to DNA STAT3 binding site of PD-L1 promoter region (unknown origin) assessed as disappearance of band of wild-type double stranded DNA-STAT3 protein complex at > 8 uM by competitive electrophoretic mobility shift assay	Not specified	2	ALA5223080	assay format	Scientific Literature
13.	ALA5223644	B	Binding affinity to 5'-biotin-double stranded DNA (unknown origin) assessed as saturation of the compound-DNA complex at 350 to 500 nM by surface plasmon resonance	Homo sapiens	2	ALA5223080	assay format	Scientific Literature
14.	ALA5223645	B	Binding affinity to 5'-biotin-double stranded DNA (unknown origin) assessed as interaction with DNA to achieve steady state at 20 nM	Not specified	1	ALA5223080	nucleic acid format	Scientific Literature
15.	ALA5223646	F	Inhibition of PD-L1 mRNA expression in IFNgamma-induced human HCC827 cells measured for 36 hrs by qRT-PCR analysis	Homo sapiens	2	ALA5223080	cell-based format	Scientific Literature
16.	ALA5223647	F	Inhibition of PD-L1 mRNA expression in IFNgamma-induced human MDA-MB-231 cells measured for 36 hrs by qRT-PCR analysis	Homo sapiens	2	ALA5223080	cell-based format	Scientific Literature
17.	ALA5223648	F	Inhibition of PD-L1 protein expression in IFNgamma-induced human HCC827 cells measured for 36 hrs by Western blot analysis	Homo sapiens	3	ALA5223080	cell-based format	Scientific Literature
18.	ALA5223649	F	Inhibition of PD-L1 protein expression in IFNgamma-induced human MDA-MB-231 cells measured for 36 hrs by Western blot analysis	Homo sapiens	3	ALA5223080	cell-based format	Scientific Literature
19.	ALA5223651	F	Induction of T-cell mediated cell killing in human HCC827 cells co-cultured with PMA-activated Jurkat T cells assessed as IL-2 production at 8 uM by ELISA relative to control	Homo sapiens	3	ALA5223080	cell-based format	Scientific Literature
20.	ALA5223652	F	Induction of T-cell mediated cell killing in human MDA-MB-231 cells co-cultured with PMA-activated Jurkat T cells assessed as IL-2 production at 8 uM by ELISA relative to control	Homo sapiens	3	ALA5223080	cell-based format	Scientific Literature