# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5256384 | Binding | Inhibition of CDK4/CDK6 signaling pathway in human MDA-MB-231 cells assessed as reduction of RB phosphorylation at Ser780 residue level at 1 to 8 uM incubated for 24 hrs by western blot assay | Homo sapiens | 1 | ALA5252647 | cell-based format | Scientific Literature | |
2. | ALA5256385 | Binding | Inhibition of CDK4/CDK6 signaling pathway in human MDA-MB-231 cells assessed as inhibition of CDK6 expression level at 1 to 8 uM incubated for 24 hrs by western blot assay | Homo sapiens | 1 | ALA5252647 | cell-based format | Scientific Literature | |
3. | ALA5256386 | Binding | Inhibition of CDK4/CDK6 signaling pathway in human MDA-MB-231 cells assessed as inhibition of CDK4 expression level at 1 to 8 uM incubated for 24 hrs by western blot assay | Homo sapiens | 1 | ALA5252647 | cell-based format | Scientific Literature | |
4. | ALA5256387 | Functional | Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay | Homo sapiens | 15 | ALA5252647 | cell-based format | Scientific Literature | |
5. | ALA5256388 | Functional | Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay | Homo sapiens | 15 | ALA5252647 | cell-based format | Scientific Literature | |
6. | ALA5256389 | Functional | Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay | Homo sapiens | 15 | ALA5252647 | cell-based format | Scientific Literature | |
7. | ALA5256390 | Functional | Antiproliferative activity against human HepG2 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay | Homo sapiens | 15 | ALA5252647 | cell-based format | Scientific Literature | |
8. | ALA5256391 | Functional | Antiproliferative activity against palbociclib-resistant human MCF7 cells assessed as inhibition of cell growth incubated for 72 hrs by MTT assay | Homo sapiens | 2 | ALA5252647 | cell-based format | Scientific Literature | |
9. | ALA5256392 | Functional | Resistance factor, ratio of IC50 for palbociclib-resistant human MCF7 cells to IC50 for human MCF7 cells | Homo sapiens | 2 | ALA5252647 | cell-based format | Scientific Literature | |
10. | ALA5256393 | Binding | Covalent inhibition of CDK4 (unknown origin) using histone H1 as substrate incubated for 10 mins in presence of [gamma-32P]ATP by radioactivity based kinase assay | Homo sapiens | 4 | ALA5252647 | single protein format | Scientific Literature | |
11. | ALA5256394 | Binding | Covalent inhibition of CDK6 (unknown origin) using histone H1 as substrate incubated for 10 mins in presence of [gamma-32P]ATP by radioactivity based kinase assay | Homo sapiens | 4 | ALA5252647 | single protein format | Scientific Literature | |
12. | ALA5256395 | Binding | Covalent inhibition of CDK9 (unknown origin) using histone H1 as substrate incubated for 10 mins in presence of [gamma-32P]ATP by radioactivity based kinase assay | Homo sapiens | 4 | ALA5252647 | single protein format | Scientific Literature | |
13. | ALA5256396 | Binding | Covalent inhibition of CDK12 (unknown origin) using histone H1 as substrate incubated for 10 mins in presence of [gamma-32P]ATP by radioactivity based kinase assay | Homo sapiens | 4 | ALA5252647 | single protein format | Scientific Literature | |
14. | ALA5256397 | Functional | Induction of apoptosis in human MDA-MB-231 cells assessed as viable cells at 10 uM incubated for 24 hrs by annexin V-FITC/propidium iodide staining based flow cytometry analysis (Rvb = 96.5%) | Homo sapiens | 4 | ALA5252647 | cell-based format | Scientific Literature | |
15. | ALA5256398 | Functional | Induction of apoptosis in human MDA-MB-231 cells assessed as early apoptotic cells at 10 uM incubated for 24 hrs by annexin V-FITC/propidium iodide staining based flow cytometry analysis (Rvb = 3.39%) | Homo sapiens | 4 | ALA5252647 | cell-based format | Scientific Literature | |
16. | ALA5256399 | Functional | Induction of apoptosis in human MDA-MB-231 cells assessed as late apoptotic cells at 10 uM incubated for 24 hrs by annexin V-FITC/propidium iodide staining based flow cytometry analysis (Rvb = 0.113%) | Homo sapiens | 4 | ALA5252647 | cell-based format | Scientific Literature | |
17. | ALA5256400 | Functional | Induction of apoptosis in human MDA-MB-231 cells assessed as necrotic cells at 10 uM incubated for 24 hrs by annexin V-FITC/propidium iodide staining based flow cytometry analysis (Rvb = 0.034%) | Homo sapiens | 4 | ALA5252647 | cell-based format | Scientific Literature | |
18. | ALA5256401 | Functional | Cell cycle arrest in human MDA-MB-231 cells assessed as accumulation of cells at G0/G1 phase at 10 uM incubated for 24 hrs by propidium iodide staining based assay (Rvb = 54.43%) | Homo sapiens | 4 | ALA5252647 | cell-based format | Scientific Literature | |
19. | ALA5256402 | Functional | Cell cycle arrest in human MDA-MB-231 cells assessed as accumulation of cells at G2/M phase at 10 uM incubated for 24 hrs by propidium iodide staining based assay (Rvb = 20.49%) | Homo sapiens | 4 | ALA5252647 | cell-based format | Scientific Literature | |
20. | ALA5256403 | Functional | Cell cycle arrest in human MDA-MB-231 cells assessed as accumulation of cells at S phase at 10 uM incubated for 24 hrs by propidium iodide staining based assay (Rvb = 25.09%) | Homo sapiens | 4 | ALA5252647 | cell-based format | Scientific Literature |