# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5251899 | B | Inhibition of N-terminal His-tagged recombinant human BTK (393 to 659 residues) expressed in baculovirus infected Sf9 cells using Biotin-AVLESEEELYSSARQ-NH2 as substrate preincubated for 1 hrs followed by substrate addition in presence of ATP at Km concentration and measured after 1 hrs by TR-FRET assay | Homo sapiens | 39 | cell-based format | Scientific Literature | ||
2. | ALA5251900 | B | Inhibition of EGFR (unknown origin) preincubated for 1 hrs followed by substrate addition and measured after 1 hrs in the presence of ATP at Km concentration by TR-FRET assay | Homo sapiens | 29 | single protein format | Scientific Literature | ||
3. | ALA5251901 | B | Inhibition of TEC (unknown origin) preincubated for 1 hrs followed by substrate addition and measured after 1 hrs in the presence of ATP at Km concentration by TR-FRET assay | Homo sapiens | 29 | single protein format | Scientific Literature | ||
4. | ALA5251902 | B | Inhibition of EGFR (unknown origin) assessed as maximum inhibition at 1000 nM preincubated for 1 hrs followed by substrate addition and measured after 1 hrs in the presence of ATP at Km concentration by TR-FRET assay relative to control | Homo sapiens | 4 | single protein format | Scientific Literature | ||
5. | ALA5251905 | B | Inhibition of BTK phosphorylation at Tyr 223 residue in human Ramos cell incubated for 3 hrs by HTRF-based analysis | Homo sapiens | 30 | cell-based format | Scientific Literature | ||
6. | ALA5251906 | F | In vivo protein occupancy at BTK in ICR mouse splenocyte at 15 mg/kg, po administered as single dose and measured after 4 hrs by ELISA analysis relative to control | Mus musculus | 6 | organism-based format | Scientific Literature |