| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5242984 | B | Inhibition of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay | Homo sapiens | 54 | ALA5241107 | cell-based format | Scientific Literature | |
| 2. | ALA5242985 | B | Uncompetitive inhibition of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells using varying concentrations of ATP and 250 ug/ml yeast RNA as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 1 to 7 hrs by Malachite green ATPase assay | Homo sapiens | 2 | ALA5241107 | cell-based format | Scientific Literature | |
| 3. | ALA5242986 | B | Uncompetitive inhibition of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells assessed as decrease in Km and Vmax of ATP using varying concentrations of ATP and 250 ug/ml yeast RNA as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 1 to 7 hrs by Malachite green ATPase assay | Homo sapiens | 2 | ALA5241107 | cell-based format | Scientific Literature | |
| 4. | ALA5242987 | B | Competitive inhibition of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells using 250 uM ATP and varying concentration of yeast RNA as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 1 to 7 hrs by Malachite green ATPase assay | Homo sapiens | 2 | ALA5241107 | cell-based format | Scientific Literature | |
| 5. | ALA5242988 | B | Competitive inhibition of human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells assessed as decrease in Km with constant Vmax of RNA using 250 uM ATP and varying concentration of yeast RNA as substrate preincubated with enzyme for 20 mins followed by substrate addition and measured after 1 to 7 hrs by Malachite green ATPase assay | Homo sapiens | 2 | ALA5241107 | cell-based format | Scientific Literature | |
| 6. | ALA5242989 | B | Inhibition of human recombinant N-terminal His6-tagged eIF4A R110L mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay | Homo sapiens | 1 | ALA5241107 | cell-based format | Scientific Literature | |
| 7. | ALA5242990 | B | Inhibition of human recombinant N-terminal His6-tagged eIF4A R110M mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay | Homo sapiens | 1 | ALA5241107 | cell-based format | Scientific Literature | |
| 8. | ALA5242991 | B | Inhibition of human recombinant N-terminal His6-tagged eIF4A R110E mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay | Homo sapiens | 1 | ALA5241107 | cell-based format | Scientific Literature | |
| 9. | ALA5242992 | B | Inhibition of human recombinant N-terminal His6-tagged eIF4A T158A mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay | Homo sapiens | 1 | ALA5241107 | cell-based format | Scientific Literature | |
| 10. | ALA5242993 | B | Inhibition of human recombinant N-terminal His6-tagged eIF4A T158V mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay | Homo sapiens | 1 | ALA5241107 | cell-based format | Scientific Literature | |
| 11. | ALA5242994 | B | Inhibition of human recombinant N-terminal His6-tagged eIF4A R311K mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay | Homo sapiens | 1 | ALA5241107 | cell-based format | Scientific Literature | |
| 12. | ALA5242995 | B | Inhibition of human recombinant N-terminal His6-tagged eIF4A R311E mutant expressed in Escherichia coli BL21 CodonPlus cells using ATP and yeast RNA as substrate preincubated with enzyme for 20 mins followed by susbtrate addition and measured after 4 hrs by Malachite green ATPase assay | Homo sapiens | 1 | ALA5241107 | cell-based format | Scientific Literature | |
| 13. | ALA5242996 | F | Cytotoxicity against human BJAB cells assessed as reduction in cell viability measured after 72 hrs by CellTiter-Glo luminescent assay | Homo sapiens | 7 | ALA5241107 | cell-based format | Scientific Literature | |
| 14. | ALA5242997 | B | Inhibition of eIF4A in rabbit reticulocyte lysate assessed as inhibition of cap-dependent translation of firefly luciferase using bicistronic luciferase reporter [pSP/(CAG)33/FF/HCV/Ren.pA51] incubated for 90 mins by luciferase reporter gene based luminescence analysis | Oryctolagus cuniculus | 1 | ALA5241107 | subcellular format | Scientific Literature | |
| 15. | ALA5242998 | F | Inhibition of IRES-dependent translation of renilla luciferase in rabbit reticulocyte lysates using bicistronic luciferase reporter [pSP/(CAG)33/FF/HCV/Ren.pA51] incubated for 90 mins by luciferase reporter gene based luminescence analysis | Oryctolagus cuniculus | 1 | ALA5241107 | subcellular format | Scientific Literature | |
| 16. | ALA5243004 | B | Binding affinity to eIF4A in human A549 cells assessed as increase in thermal stability of the protein incubated for 90 mins by CETSA analysis | Homo sapiens | 1 | ALA5241107 | cell-based format | Scientific Literature | |
| 17. | ALA5243005 | B | Displacement of MANT-ATP from human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells at 200 uM preincubated with enzyme and yeast RNA for 20 mins followed by MANT-ATP addition for 30 mins by fluorescence polarization assay | Homo sapiens | 1 | ALA5241107 | cell-based format | Scientific Literature | |
| 18. | ALA5243007 | B | Displacement of FAM-A-[CAA]5 from human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells preincubated with enzyme for 20 mins followed by FAM-A[CAA]5 addition for 30 mins by fluorescence polarization assay | Homo sapiens | 2 | ALA5241107 | cell-based format | Scientific Literature | |
| 19. | ALA5243008 | B | Displacement of FAM-[AG]8 from human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells preincubated with enzyme for 20 mins followed by FAM-[AG]8 addition for 30 mins by fluorescence polarization assay | Homo sapiens | 1 | ALA5241107 | cell-based format | Scientific Literature | |
| 20. | ALA5243009 | B | Binding affinity to human recombinant N-terminal His6-tagged eIF4A expressed in Escherichia coli BL21 CodonPlus cells assessed as increase in melting temperature at 3.13 to 50 uM incubated for 5 mins by differential scanning fluorimetry | Homo sapiens | 1 | ALA5241107 | cell-based format | Scientific Literature |
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