# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5228924 | Binding | Binding affinity to biotinylated USP5 zinc finger ubiquitin binding domain (171 to 290 residues) (unknown origin) expressed in Escherichia coli by surface plasmon resonance assay | Homo sapiens | 56 | ALA5226343 | assay format | Scientific Literature | |
2. | ALA5228925 | Binding | Binding affinity to biotinylated HDAC6 zinc finger ubiquitin binding domain (1109 to 1215 residues) (unknown origin) expressed in Escherichia coli by surface plasmon resonance assay | Homo sapiens | 55 | ALA5226343 | assay format | Scientific Literature | |
3. | ALA5228926 | Binding | Selectivity ratio of Kd for biotinylated HDAC6 zinc finger ubiquitin binding domain (1109 to 1215 residues) (unknown origin) expressed in Escherichia coli to Kd for biotinylated USP5 zinc finger ubiquitin binding domain (171 to 290 residues) (unknown origin) expressed in Escherichia coli by surface plasmon resonance assay | Homo sapiens | 45 | ALA5226343 | assay format | Scientific Literature | |
4. | ALA5228927 | Binding | Binding affinity to full length biotinylated USP5 (1 to 835 residues) (unknown origin) expressed in Escherichia coli by surface plasmon resonance assay | Homo sapiens | 1 | ALA5226343 | assay format | Scientific Literature | |
5. | ALA5228928 | Binding | Selectivity ratio of Kd for full length biotinylated USP5 (1 to 835 residues) (unknown origin) expressed in Escherichia coli to Kd for biotinylated USP5 zinc finger ubiquitin binding domain (171 to 290 residues) (unknown origin) expressed in Escherichia coli by surface plasmon resonance assay | Homo sapiens | 1 | ALA5226343 | assay format | Scientific Literature | |
6. | ALA5228929 | Binding | Binding affinity to full length biotinylated USP5 R221A mutant (unknown origin) expressed in Escherichia coli by surface plasmon resonance assay | Homo sapiens | 1 | ALA5226343 | assay format | Scientific Literature | |
7. | ALA5228930 | Binding | Displacement of N-terminal FITC-labeled Ub from full length biotinylated USP5 (unknown origin) expressed in Escherichia coli incubated for 1 hr by fluorescence competition assay | Homo sapiens | 1 | ALA5226343 | assay format | Scientific Literature | |
8. | ALA5228931 | Binding | Displacement of N-terminal FITC-labeled Ub from biotinylated USP5 zinc finger ubiquitin binding domain (171 to 290 residues) (unknown origin) expressed in Escherichia coli incubated for 1 hr by fluorescence competition assay | Homo sapiens | 1 | ALA5226343 | assay format | Scientific Literature | |
9. | ALA5228932 | Binding | Selectivity ratio of IC50 for displacement of N-terminal FITC-labeled Ub from full length biotinylated USP5 (unknown origin) expressed in Escherichia coli to IC50 for displacement of N-terminal FITC-labeled Ub from biotinylated USP5 zinc finger ubiquitin binding domain (171 to 290 residues) (unknown origin) expressed in Escherichia coli by fluorescence competition assay | Homo sapiens | 1 | ALA5226343 | assay format | Scientific Literature | |
10. | ALA5228933 | Binding | Inhibition of full length USP5 (1 to 835 residues) (unknown origin) expressed in Escherichia coli using DU48-02 as substrate preincubated with enzyme for 1 hr followed by substrate addition and measured for 10 mins by IQF assay | Homo sapiens | 1 | ALA5226343 | assay format | Scientific Literature | |
11. | ALA5228934 | Binding | Inhibition of full length USP5 (1 to 835 residues) (unknown origin) expressed in Escherichia coli using DU48-03 as substrate preincubated with enzyme for 1 hr followed by substrate addition and measured for 10 mins by IQF assay | Homo sapiens | 1 | ALA5226343 | assay format | Scientific Literature | |
12. | ALA5228935 | Binding | Competitive inhibition of full length USP5 (1 to 835 residues) (unknown origin) expressed in Escherichia coli using DU48-03 as substrate preincubated with enzyme for 1 hr followed by varying concentrations of substrate addition and measured for 10 mins by IQF assay | Homo sapiens | 1 | ALA5226343 | assay format | Scientific Literature | |
13. | ALA5228962 | ADME | Metabolic stability of the compound in human liver microsomes assessed as parent compound remaining at 1 uM measured for 60 mins in presence of NADPH regenerating system by LC/MS analysis | Homo sapiens | 1 | ALA5226343 | microsome format | Scientific Literature | |
14. | ALA5228963 | ADME | Metabolic stability of the compound in CD-1 mouse liver microsomes assessed as parent compound remaining at 1 uM measured after 60 mins in presence of NADPH regenerating system by LC/MS analysis | Mus musculus | 1 | ALA5226343 | microsome format | Scientific Literature |