#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5225647	B	Inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using L-malate and beta-NADP+ as substrate preincubated with enzyme for 5 mins followed by beta-NADP+ challenge and incubated under shaking for 5 mins followed by incubation for 3 hrs without shaking by resorufin dye based fluorescence analysis	Homo sapiens	34	ALA5223163	single protein format	Scientific Literature
2.	ALA5225648	B	Inhibition of His-tagged human recombinant full length ME2 using L-malate and beta-NAD+ as substrate preincubated with enzyme for 5 mins followed by beta-NAD+ challenge and incubated under shaking for 5 mins followed by incubation for 2 hrs without shaking by resorufin dye based fluorescence analysis	Homo sapiens	32	ALA5223163	single protein format	Scientific Literature
3.	ALA5225649	B	Inhibition of His-tagged human recombinant full length ME1 using L-malate and beta-NADP+ as substrate preincubated with enzyme for 5 mins followed by beta-NADP+ challenge and incubated under shaking for 5 mins followed by incubation for 2 hrs without shaking by resorufin dye based fluorescence analysis	Homo sapiens	32	ALA5223163	single protein format	Scientific Literature
4.	ALA5225650	F	Antiproliferative activity against SMAD4/ME2-null human BXPC-3 cells assessed as inhibition of cell growth at 10 uM incubated for 72 hrs by CellTiter-Glo luminescence assay	Homo sapiens	31	ALA5223163	cell-based format	Scientific Literature
5.	ALA5225651	F	Antiproliferative activity against SMAD4/ME2-null human BXPC-3 cells assessed as inhibition of cell growth incubated for 72 hrs by CellTiter-Glo luminescence assay	Homo sapiens	14	ALA5223163	cell-based format	Scientific Literature
6.	ALA5225652	B	Inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using L-malate and beta-NADP+ as substrate at 1 uM preincubated with enzyme for 5 mins followed by beta-NADP+ challenge and incubated under shaking for 5 mins followed by incubation for 3 hrs without shaking by resorufin dye based fluorescence analysis	Homo sapiens	16	ALA5223163	single protein format	Scientific Literature
7.	ALA5225653	B	Inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using L-malate and beta-NADP+ as substrate at 10 uM preincubated with enzyme for 5 mins followed by beta-NADP+ challenge and incubated under shaking for 5 mins followed by incubation for 3 hrs without shaking by resorufin dye based fluorescence analysis	Homo sapiens	11	ALA5223163	single protein format	Scientific Literature
8.	ALA5225656	B	Competitive inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using varying concentrations of L-malate as substrate in presence of fixed concentration of beta-NADP+ and measured for 10 mins by Lineweaver-Burk plot analysis	Homo sapiens	1	ALA5223163	single protein format	Scientific Literature
9.	ALA5225657	B	Binding affinity to ME3 in human BXPC-3 live cells assessed as change in melting temperature at 100 uM preincubated with live cells for 24 hrs followed by exposure to varying temperature by CETSA analysis	Homo sapiens	1	ALA5223163	cell-based format	Scientific Literature
10.	ALA5225658	B	Uncompetitive inhibition of His-tagged human recombinant ME3 (26 to 604 residues) using varying concentrations of beta-NADP+ as substrate in presence of fixed concentration of L-malate and measured for 10 mins by Lineweaver-Burk plot analysis	Homo sapiens	1	ALA5223163	single protein format	Scientific Literature
11.	ALA5225659	B	Binding affinity to ME3 in human BXPC-3 cell lysates assessed as change in melting temperature at 100 uM preincubated with cell lysates for 2 hrs followed by exposure to varying temperature by CETSA analysis	Homo sapiens	1	ALA5223163	subcellular format	Scientific Literature
12.	ALA5225663	T	Cytotoxicity against human HCE-T cells assessed as inhibition of cell growth	Homo sapiens	8	ALA5223163	cell-based format	Scientific Literature
13.	ALA5225664	T	Cytotoxicity against human HUVEC cells assessed as inhibition of cell growth	Homo sapiens	2	ALA5223163	cell-based format	Scientific Literature
14.	ALA5225665	U	Selectivity for SMAD4/ME2-null human BXPC-3 cells over human HCE-T cells		24	ALA5223163	assay format	Scientific Literature