| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5229239 | B | Inhibition of Mycobacterium tuberculosis recombinant PknB (1 to 331 residues) expressed in Escherichia coli BL21-Rosetta cells using GarA and ATP as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured after 1 hr by ATP-competitive biochemical assay | Mycobacterium tuberculosis | 18 | cell-based format | Scientific Literature | ||
| 2. | ALA5229240 | B | Binding affinity to Mycobacterium tuberculosis recombinant N-terminal His-tagged PknB (1 to 331 residues) expressed in Escherichia coli DH5alpha cells by microscale thermophoresis analysis | Mycobacterium tuberculosis | 18 | cell-based format | Scientific Literature | ||
| 3. | ALA5229241 | T | Inhibition of human Cdk2/Cyclin A using Myelin basic protein as substrate by ATP competitive assay | Homo sapiens | 18 | assay format | Scientific Literature | ||
| 4. | ALA5229242 | B | Binding affinity to Mycobacterium tuberculosis partial length PknB (M1 to G279 residues) expressed in mammalian expression system by KdELECT kinase assay | Mycobacterium tuberculosis | 5 | assay format | Scientific Literature | ||
| 5. | ALA5229243 | T | Binding affinity to human full length CDK4 (M1 to E303 residues) expressed in mammalian expression system/CyclinD3 by KdELECT kinase assay | Homo sapiens | 5 | assay format | Scientific Literature | ||
| 6. | ALA5229244 | T | Binding affinity to human full length GSK-3 beta (M1 to T433 residues) expressed in mammalian expression system by KdELECT kinase assay | Homo sapiens | 5 | assay format | Scientific Literature | ||
| 7. | ALA5229245 | T | Binding affinity to human partial length mTOR (L1382 to W2549 residues) expressed in mammalian expression system by KdELECT kinase assay | Homo sapiens | 5 | assay format | Scientific Literature | ||
| 8. | ALA5229246 | T | Binding affinity to human partial length Pim1 (A15 to K313 residues ) expressed in bacterial expression system by KdELECT kinase assay | Homo sapiens | 5 | assay format | Scientific Literature | ||
| 9. | ALA5229247 | B | Selectivity ratio of Kd for human full length CDK4 (M1 to E303 residues) expressed in mammalian expression system/CyclinD3 to Kd for Mycobacterium tuberculosis partial length PknB (M1 to G279 residues) expressed in mammalian expression system | 1 | assay format | Scientific Literature | |||
| 10. | ALA5229248 | B | Selectivity ratio of Kd for human full length GSK-3 beta (M1 to T433 residues) expressed in mammalian expression system to Kd for Mycobacterium tuberculosis partial length PknB (M1 to G279 residues) expressed in mammalian expression system | 1 | assay format | Scientific Literature | |||
| 11. | ALA5229249 | B | Selectivity ratio of Kd for human partial length mTOR (L1382 to W2549 residues) expressed in mammalian expression system to Kd for Mycobacterium tuberculosis partial length PknB (M1 to G279 residues) expressed in mammalian expression system | 1 | assay format | Scientific Literature | |||
| 12. | ALA5229250 | B | Selectivity ratio of Kd for human partial length Pim1 (A15 to K313 residues ) expressed in bacterial expression system to Kd for Mycobacterium tuberculosis partial length PknB (M1 to G279 residues) expressed in mammalian expression system | 1 | assay format | Scientific Literature | |||
| 13. | ALA5229251 | F | Antimycobacterial activity against Mycobacterium tuberculosis H37Ra ATCC 25177 assessed as inhibition of bacterial growth incubated for 12 days by Alamar Blue based fluorescence analysis | Mycobacterium tuberculosis | 9 | organism-based format | Scientific Literature | ||
| 14. | ALA5229252 | F | Antimycobacterial activity against Mycobacterium tuberculosis H37Rv assessed as inhibition of bacterial growth incubated for 12 days by Alamar Blue based fluorescence analysis | Mycobacterium tuberculosis | 9 | organism-based format | Scientific Literature | ||
| 15. | ALA5229253 | P | Lipophilicity, logD of the compound in octanol and phosphate buffer at pH 7.4 at 66.7 uM by LC-MS/MS analysis | 9 | small-molecule physicochemical format | Scientific Literature | |||
| 16. | ALA5229254 | T | Cytotoxicity against human THP-1 cells assessed as reduction in cell viability incubated for 72 hrs by CellTiter-Glo luminescent assay | Homo sapiens | 9 | cell-based format | Scientific Literature | ||
| 17. | ALA5229255 | T | Toxicity against Zebrafish embryo assessed as embryo survival at 5 uM incubated for 5 days and measured daily | Danio rerio | 9 | assay format | Scientific Literature |
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