#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5229239	B	Inhibition of Mycobacterium tuberculosis recombinant PknB (1 to 331 residues) expressed in Escherichia coli BL21-Rosetta cells using GarA and ATP as substrate preincubated with enzyme for 10 mins followed by substrate addition and measured after 1 hr by ATP-competitive biochemical assay	Mycobacterium tuberculosis	18	ALA5226357	cell-based format	Scientific Literature
2.	ALA5229240	B	Binding affinity to Mycobacterium tuberculosis recombinant N-terminal His-tagged PknB (1 to 331 residues) expressed in Escherichia coli DH5alpha cells by microscale thermophoresis analysis	Mycobacterium tuberculosis	18	ALA5226357	cell-based format	Scientific Literature
3.	ALA5229241	T	Inhibition of human Cdk2/Cyclin A using Myelin basic protein as substrate by ATP competitive assay	Homo sapiens	18	ALA5226357	assay format	Scientific Literature
4.	ALA5229242	B	Binding affinity to Mycobacterium tuberculosis partial length PknB (M1 to G279 residues) expressed in mammalian expression system by KdELECT kinase assay	Mycobacterium tuberculosis	5	ALA5226357	assay format	Scientific Literature
5.	ALA5229243	T	Binding affinity to human full length CDK4 (M1 to E303 residues) expressed in mammalian expression system/CyclinD3 by KdELECT kinase assay	Homo sapiens	5	ALA5226357	assay format	Scientific Literature
6.	ALA5229244	T	Binding affinity to human full length GSK-3 beta (M1 to T433 residues) expressed in mammalian expression system by KdELECT kinase assay	Homo sapiens	5	ALA5226357	assay format	Scientific Literature
7.	ALA5229245	T	Binding affinity to human partial length mTOR (L1382 to W2549 residues) expressed in mammalian expression system by KdELECT kinase assay	Homo sapiens	5	ALA5226357	assay format	Scientific Literature
8.	ALA5229246	T	Binding affinity to human partial length Pim1 (A15 to K313 residues ) expressed in bacterial expression system by KdELECT kinase assay	Homo sapiens	5	ALA5226357	assay format	Scientific Literature
9.	ALA5229247	B	Selectivity ratio of Kd for human full length CDK4 (M1 to E303 residues) expressed in mammalian expression system/CyclinD3 to Kd for Mycobacterium tuberculosis partial length PknB (M1 to G279 residues) expressed in mammalian expression system		1	ALA5226357	assay format	Scientific Literature
10.	ALA5229248	B	Selectivity ratio of Kd for human full length GSK-3 beta (M1 to T433 residues) expressed in mammalian expression system to Kd for Mycobacterium tuberculosis partial length PknB (M1 to G279 residues) expressed in mammalian expression system		1	ALA5226357	assay format	Scientific Literature
11.	ALA5229249	B	Selectivity ratio of Kd for human partial length mTOR (L1382 to W2549 residues) expressed in mammalian expression system to Kd for Mycobacterium tuberculosis partial length PknB (M1 to G279 residues) expressed in mammalian expression system		1	ALA5226357	assay format	Scientific Literature
12.	ALA5229250	B	Selectivity ratio of Kd for human partial length Pim1 (A15 to K313 residues ) expressed in bacterial expression system to Kd for Mycobacterium tuberculosis partial length PknB (M1 to G279 residues) expressed in mammalian expression system		1	ALA5226357	assay format	Scientific Literature
13.	ALA5229251	F	Antimycobacterial activity against Mycobacterium tuberculosis H37Ra ATCC 25177 assessed as inhibition of bacterial growth incubated for 12 days by Alamar Blue based fluorescence analysis	Mycobacterium tuberculosis	9	ALA5226357	organism-based format	Scientific Literature
14.	ALA5229252	F	Antimycobacterial activity against Mycobacterium tuberculosis H37Rv assessed as inhibition of bacterial growth incubated for 12 days by Alamar Blue based fluorescence analysis	Mycobacterium tuberculosis	9	ALA5226357	organism-based format	Scientific Literature
15.	ALA5229253	P	Lipophilicity, logD of the compound in octanol and phosphate buffer at pH 7.4 at 66.7 uM by LC-MS/MS analysis		9	ALA5226357	small-molecule physicochemical format	Scientific Literature
16.	ALA5229254	T	Cytotoxicity against human THP-1 cells assessed as reduction in cell viability incubated for 72 hrs by CellTiter-Glo luminescent assay	Homo sapiens	9	ALA5226357	cell-based format	Scientific Literature
17.	ALA5229255	T	Toxicity against Zebrafish embryo assessed as embryo survival at 5 uM incubated for 5 days and measured daily	Danio rerio	9	ALA5226357	assay format	Scientific Literature