| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5225583 | B | Inhibition of mPGES-1 activity in IL-1beta stimulated human A549 microsomal preparation using PGH2 as substrate assessed as inhibition of PGE2 synthesis by measuring residual activity at 10 uM preincubated for 15 mins followed by substrate addition and measured after 1 min by RP-HPLC analysis relative to control | Homo sapiens | 9 | microsome format | Scientific Literature | ||
| 2. | ALA5225584 | B | Inhibition of mPGES-1 activity in IL-1beta stimulated human A549 microsomal preparation using PGH2 as substrate assessed as inhibition of PGE2 synthesis preincubated for 15 mins followed by substrate addition and measured after 1 min by RP-HPLC analysis | Homo sapiens | 9 | microsome format | Scientific Literature | ||
| 3. | ALA5225585 | B | Inhibition of ovine COX-1 using arachidonic acid as substrate assessed as reduction in 12-HHT formation by measuring residual activity at 10 uM preincubated for 5 mins followed by substrate addition and measured after 5 mins by RP-HPLC analysis relative to control | Ovis aries | 4 | single protein format | Scientific Literature | ||
| 4. | ALA5225586 | B | Inhibition of human COX-2 using arachidonic acid as substrate assessed as reduction in 12-HHT formation by measuring residual activity at 10 uM preincubated for 5 mins followed by substrate addition and measured after 5 mins by RP-HPLC analysis relative to control | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 5. | ALA5225587 | B | Inhibition of human recombinant 5-LO expressed in Escherichia coli BL21 using arachidonic acid as substrate assessed as residual activity at 10 uM preincubated for 10 mins followed by substrate addition and measured after 10 mins by RP-HPLC analysis relative to control | Homo sapiens | 2 | assay format | Scientific Literature | ||
| 6. | ALA5225589 | B | Inhibition of recombinant human sEH expressed in baculovirus infected Sf9 cells using PHOME as substrate assessed as residual activity at 10 uM preincubated for 15 mins followed by substrate addition and measured after 60 mins by spectrofluorimetric method relative to control | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 7. | ALA5225591 | F | Inhibition of Leukotriene biosynthesis in human PMNL cells preincubated for 10 mins followed by Ca2+-ionophore A23187 addition and measured after 10 mins by RP-HPLC analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 8. | ALA5225592 | F | Inhibition of Leukotriene biosynthesis in human PMNL cells preincubated for 10 mins followed by Ca2+-ionophore A23187 and arachidonic acid addition and measured after 10 mins by RP-HPLC analysis | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 9. | ALA5225593 | F | Inhibition of 5-LO product formation in Staphylococcus aureus activated human monocyte derived macrophages at 10 uM preincubated for 15 mins followed by Staphylococcus aureus activation for 90 mins by UPLC-MS/MS analysis | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
| 10. | ALA5225594 | F | Modulation of IL-6 production in LPS-stimulated mouse J774.A1 cells at 1 uM incubated for 24 hrs by ELISA | Mus musculus | 2 | cell-based format | Scientific Literature | ||
| 11. | ALA5225595 | F | Modulation of PGE2 production in LPS-stimulated mouse J774.A1 cells at 1 uM incubated for 24 hrs by ELISA | Mus musculus | 2 | cell-based format | Scientific Literature | ||
| 12. | ALA5225596 | F | Anti-inflammatory activity against CD-1 mouse model of zymosan-induced peritonitis assessed as reduction in leucocyte recruitment in lavage fluid at 1 to 10 mg/kg, ip pretreated with zymosan for 30 mins followed by compound addition and measured after 4 to 24 hrs by TC10 automated cell counter analysis | Mus musculus | 1 | organism-based format | Scientific Literature | ||
| 13. | ALA5225598 | F | Anti-inflammatory activity against CD-1 mouse model of zymosan-induced peritonitis assessed as reduction in IL-1beta level at 10 mg/kg, ip pretreated with zymosan for 30 mins followed by compound addition and measured after 4 to 24 hrs by ELISA | Mus musculus | 1 | organism-based format | Scientific Literature | ||
| 14. | ALA5225599 | F | Anti-inflammatory activity against CD-1 mouse model of zymosan-induced peritonitis assessed as reduction in IL-6 level at 10 mg/kg, ip pretreated with zymosan for 30 mins followed by compound addition and measured after 4 to 24 hrs by ELISA | Mus musculus | 1 | organism-based format | Scientific Literature | ||
| 15. | ALA5225600 | F | Anti-inflammatory activity against CD-1 mouse model of zymosan-induced peritonitis assessed as reduction in PGE2 level at 10 mg/kg, ip pretreated with zymosan for 30 mins followed by compound addition and measured after 4 to 24 hrs by ELISA | Mus musculus | 1 | organism-based format | Scientific Literature | ||
| 16. | ALA5225601 | F | Anti-inflammatory activity against CD-1 mouse model of zymosan-induced peritonitis assessed as reduction in IL-10 level at 10 mg/kg, ip pretreated with zymosan for 30 mins followed by compound addition and measured after 24 hrs by ELISA | Mus musculus | 1 | organism-based format | Scientific Literature |
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