#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5251097	Binding	Inhibition of human full length USP21 using Btn-Ahx-PNIRFLD-K(Ubi)-LPQQT-GD-amide as substrate preincubated for 15 to 20 mins followed by substrate addition and measured after 25 mins by HTRF assay	Homo sapiens	22	ALA5247643	single protein format	Scientific Literature
2.	ALA5251098	Binding	Inhibition of human USP21 using ubiquitin rhodamine 110 110 as substrate preincubated for 15 to 20 mins followed by enzyme addition and measured after 25 mins by fluorometric assay	Homo sapiens	22	ALA5247643	single protein format	Scientific Literature
3.	ALA5251115	Physicochemical	Lipophilicity, logD of compound at pH 7.5 by HPCL analysis		22	ALA5247643	small-molecule physicochemical format	Scientific Literature
4.	ALA5251116	Physicochemical	Aqueous solubility of compound in PBS at pH 6.5 incubated for 24 hrs by HPLC-MS/MS analysis		12	ALA5247643	small-molecule physicochemical format	Scientific Literature
5.	ALA5251119	Binding	Inhibition of full-length human USP21 transfected in human HEK293T cells co-transfected with NF-kappaB reporter firefly luciferase plasmid assessed as NF-kappaB activation at 10 uM and measured after 20 hrs by dual-luciferase reporter gene assay	Homo sapiens	4	ALA5247643	cell-based format	Scientific Literature
6.	ALA5251120	Binding	Binding affinity to C-terminally NanoLuc HiBiT-tagged USP21 transfected in human HEK293T cells assessed as increase in thermal stabilization at 49 degreeC at 50 to 100 uM incubated for 1 hrs by Nano-Glo substrate based CESTA analysis relative to control	Homo sapiens	5	ALA5247643	cell-based format	Scientific Literature
7.	ALA5251121	ADME	Apparent permeability across apical to basolateral side in human Caco-2 cells at 2 uM and measured for 2 hrs by LC-MS/MS analysis	Homo sapiens	6	ALA5247643	cell-based format	Scientific Literature
8.	ALA5251122	ADME	Efflux ratio of apparent permeability across basolateral to apical side over apical to basolateral side in human Caco-2 cells at 2 uM and measured for 2 hrs by LC-MS/MS analysis	Homo sapiens	6	ALA5247643	cell-based format	Scientific Literature
9.	ALA5251125	Binding	Binding affinity to C-terminally NanoLuc HiBiT-tagged USP21 transfected in human HEK293T cells assessed as melting temperature at 10 uM incubated for 1 hrs by CESTA analysis	Homo sapiens	2	ALA5247643	cell-based format	Scientific Literature
10.	ALA5251126	Binding	Binding affinity to C-terminally NanoLuc HiBiT-tagged USP21 transfected in human HEK293T cells ssessed as thermal stability at 49 degreeC incubated for 1 hrs by CESTA analysis	Homo sapiens	2	ALA5247643	cell-based format	Scientific Literature
11.	ALA5251127	Binding	Inhibition of full-length human USP21 transfected in human HEK293T cells co-transfected with NF-kappaB reporter firefly luciferase plasmid assessed as NF-kappaB activation and measured after 20 hrs by dual-luciferase reporter gene assay	Homo sapiens	2	ALA5247643	cell-based format	Scientific Literature
12.	ALA5251128	Functional	Antiproliferative activity against human Jurkat cells assessed as reduction in cell viability up to 30 uM incubated for 72 hrs by CellTiter-Glo luminescent assay	Homo sapiens	2	ALA5247643	cell-based format	Scientific Literature
13.	ALA5251129	Functional	Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell viability up to 30 uM incubated for 72 hrs by CellTiter-Glo luminescent assay	Homo sapiens	2	ALA5247643	cell-based format	Scientific Literature
14.	ALA5251130	Functional	Antiproliferative activity against human A549 cells assessed as reduction in cell viability up to 30 uM incubated for 72 hrs by CellTiter-Glo luminescent assay	Homo sapiens	2	ALA5247643	cell-based format	Scientific Literature
15.	ALA5251131	Functional	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability up to 30 uM incubated for 72 hrs by CellTiter-Glo luminescent assay	Homo sapiens	2	ALA5247643	cell-based format	Scientific Literature
16.	ALA5251132	Functional	Antiproliferative activity against human U2OS cells assessed as reduction in cell viability up to 30 uM incubated for 72 hrs by CellTiter-Glo luminescent assay	Homo sapiens	2	ALA5247643	cell-based format	Scientific Literature
17.	ALA5251133	Binding	Inhibition of human USP21 using ubiquitin rhodamine 110 as substrate at 10 to 50 uM by fluorometric assay	Homo sapiens	2	ALA5247643	single protein format	Scientific Literature
18.	ALA5251134	Binding	Inhibition of human USP10 using ubiquitin rhodamine 110 as substrate at 10 to 50 uM by fluorometric assay	Homo sapiens	2	ALA5247643	single protein format	Scientific Literature
19.	ALA5251135	Binding	Inhibition of human USP22 using ubiquitin rhodamine 110 as substrate at 10 to 50 uM by fluorometric assay	Homo sapiens	2	ALA5247643	single protein format	Scientific Literature
20.	ALA5251136	Binding	Inhibition of human USP2 using ubiquitin rhodamine 110 as substrate at 10 to 50 uM by fluorometric assay	Homo sapiens	1	ALA5247643	single protein format	Scientific Literature