#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5251097	B	Inhibition of human full length USP21 using Btn-Ahx-PNIRFLD-K(Ubi)-LPQQT-GD-amide as substrate preincubated for 15 to 20 mins followed by substrate addition and measured after 25 mins by HTRF assay	Homo sapiens	22		single protein format	Scientific Literature
2.	ALA5251098	B	Inhibition of human USP21 using ubiquitin rhodamine 110 110 as substrate preincubated for 15 to 20 mins followed by enzyme addition and measured after 25 mins by fluorometric assay	Homo sapiens	22		single protein format	Scientific Literature
3.	ALA5251115	P	Lipophilicity, logD of compound at pH 7.5 by HPCL analysis		22		small-molecule physicochemical format	Scientific Literature
4.	ALA5251116	P	Aqueous solubility of compound in PBS at pH 6.5 incubated for 24 hrs by HPLC-MS/MS analysis		12		small-molecule physicochemical format	Scientific Literature
5.	ALA5251119	B	Inhibition of full-length human USP21 transfected in human HEK293T cells co-transfected with NF-kappaB reporter firefly luciferase plasmid assessed as NF-kappaB activation at 10 uM and measured after 20 hrs by dual-luciferase reporter gene assay	Homo sapiens	4		cell-based format	Scientific Literature
6.	ALA5251120	B	Binding affinity to C-terminally NanoLuc HiBiT-tagged USP21 transfected in human HEK293T cells assessed as increase in thermal stabilization at 49 degreeC at 50 to 100 uM incubated for 1 hrs by Nano-Glo substrate based CESTA analysis relative to control	Homo sapiens	5		cell-based format	Scientific Literature
7.	ALA5251121	A	Apparent permeability across apical to basolateral side in human Caco-2 cells at 2 uM and measured for 2 hrs by LC-MS/MS analysis	Homo sapiens	6		cell-based format	Scientific Literature
8.	ALA5251122	A	Efflux ratio of apparent permeability across basolateral to apical side over apical to basolateral side in human Caco-2 cells at 2 uM and measured for 2 hrs by LC-MS/MS analysis	Homo sapiens	6		cell-based format	Scientific Literature
9.	ALA5251125	B	Binding affinity to C-terminally NanoLuc HiBiT-tagged USP21 transfected in human HEK293T cells assessed as melting temperature at 10 uM incubated for 1 hrs by CESTA analysis	Homo sapiens	2		cell-based format	Scientific Literature
10.	ALA5251126	B	Binding affinity to C-terminally NanoLuc HiBiT-tagged USP21 transfected in human HEK293T cells ssessed as thermal stability at 49 degreeC incubated for 1 hrs by CESTA analysis	Homo sapiens	2		cell-based format	Scientific Literature
11.	ALA5251127	B	Inhibition of full-length human USP21 transfected in human HEK293T cells co-transfected with NF-kappaB reporter firefly luciferase plasmid assessed as NF-kappaB activation and measured after 20 hrs by dual-luciferase reporter gene assay	Homo sapiens	2		cell-based format	Scientific Literature
12.	ALA5251128	F	Antiproliferative activity against human Jurkat cells assessed as reduction in cell viability up to 30 uM incubated for 72 hrs by CellTiter-Glo luminescent assay	Homo sapiens	2		cell-based format	Scientific Literature
13.	ALA5251129	F	Antiproliferative activity against human MOLM-13 cells assessed as reduction in cell viability up to 30 uM incubated for 72 hrs by CellTiter-Glo luminescent assay	Homo sapiens	2		cell-based format	Scientific Literature
14.	ALA5251130	F	Antiproliferative activity against human A549 cells assessed as reduction in cell viability up to 30 uM incubated for 72 hrs by CellTiter-Glo luminescent assay	Homo sapiens	2		cell-based format	Scientific Literature
15.	ALA5251131	F	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability up to 30 uM incubated for 72 hrs by CellTiter-Glo luminescent assay	Homo sapiens	2		cell-based format	Scientific Literature
16.	ALA5251132	F	Antiproliferative activity against human U2OS cells assessed as reduction in cell viability up to 30 uM incubated for 72 hrs by CellTiter-Glo luminescent assay	Homo sapiens	2		cell-based format	Scientific Literature
17.	ALA5251133	B	Inhibition of human USP21 using ubiquitin rhodamine 110 as substrate at 10 to 50 uM by fluorometric assay	Homo sapiens	2		single protein format	Scientific Literature
18.	ALA5251134	B	Inhibition of human USP10 using ubiquitin rhodamine 110 as substrate at 10 to 50 uM by fluorometric assay	Homo sapiens	2		single protein format	Scientific Literature
19.	ALA5251135	B	Inhibition of human USP22 using ubiquitin rhodamine 110 as substrate at 10 to 50 uM by fluorometric assay	Homo sapiens	2		single protein format	Scientific Literature
20.	ALA5251136	B	Inhibition of human USP2 using ubiquitin rhodamine 110 as substrate at 10 to 50 uM by fluorometric assay	Homo sapiens	1		single protein format	Scientific Literature