#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5239838	B	Displacement of [3H]N-methylscopolamine from human muscarinic M1 receptor expressed in human HEK293T cell membranes by radioligand competition binding based analysis	Homo sapiens	20	ALA5236624	cell membrane format	Scientific Literature
2.	ALA5239839	B	Displacement of [3H]N-methylscopolamine from human muscarinic M2 receptor expressed in human HEK293T cell membranes by radioligand competition binding based analysis	Homo sapiens	20	ALA5236624	cell membrane format	Scientific Literature
3.	ALA5239840	B	Displacement of [3H]N-methylscopolamine from human muscarinic M3 receptor expressed in human HEK293T cell membranes by radioligand competition binding based analysis	Homo sapiens	20	ALA5236624	cell membrane format	Scientific Literature
4.	ALA5239841	B	Displacement of [3H]N-methylscopolamine from human muscarinic M4 receptor expressed in human HEK293T cell membranes by radioligand competition binding based analysis	Homo sapiens	10	ALA5236624	cell membrane format	Scientific Literature
5.	ALA5239842	B	Displacement of [3H]N-methylscopolamine from human muscarinic M5 receptor expressed in human HEK293T cell membranes by radioligand competition binding based analysis	Homo sapiens	10	ALA5236624	cell membrane format	Scientific Literature
6.	ALA5239843	B	Selectivity ratio, Ki for displacement of [3H]N-methylscopolamine from human muscarinic M1 receptor expressed in human HEK293T cell membranes to Ki for displacement of [3H]N-methylscopolamine from human muscarinic M3 receptor expressed in human HEK293T cell membranes	Homo sapiens	20	ALA5236624	cell-based format	Scientific Literature
7.	ALA5239844	B	Selectivity ratio, Ki for displacement of [3H]N-methylscopolamine from human muscarinic M2 receptor expressed in human HEK293T cell membranes to Ki for displacement of [3H]N-methylscopolamine from human muscarinic M3 receptor expressed in human HEK293T cell membranes	Homo sapiens	20	ALA5236624	cell-based format	Scientific Literature
8.	ALA5239845	B	Selectivity ratio, Ki for displacement of [3H]N-methylscopolamine from human muscarinic M4 receptor expressed in human HEK293T cell membranes to Ki for displacement of [3H]N-methylscopolamine from human muscarinic M3 receptor expressed in human HEK293T cell membranes	Homo sapiens	10	ALA5236624	cell-based format	Scientific Literature
9.	ALA5239846	B	Selectivity ratio, Ki for displacement of [3H]N-methylscopolamine from human muscarinic M5 receptor expressed in human HEK293T cell membranes to Ki for displacement of [3H]N-methylscopolamine from human muscarinic M3 receptor expressed in human HEK293T cell membranes	Homo sapiens	10	ALA5236624	cell-based format	Scientific Literature
10.	ALA5239847	B	Binding affinity to human muscarinic M3 receptor expressed in human HEK293T cells assessed as inhibition of carbachol-induced G-protein signaling by measuring inositol accumulation preincubated for 30 mins followed by carbachol stimulation and measured after 90 mins by HTRF assay	Homo sapiens	2	ALA5236624	cell-based format	Scientific Literature
11.	ALA5239848	B	Binding affinity to human muscarinic M3 receptor expressed in human HEK293T cells cells co-expressing beta-arrestin2 assessed as inhibition of carbachol-induced beta-arrestin recruitment preincubated for 30 mins followed by carbachol stimulation and measured after 90 mins by PathHunter assay	Homo sapiens	2	ALA5236624	cell-based format	Scientific Literature
12.	ALA5239851	B	Binding affinity to saturated human muscarinic M3 expressed in CHO cells assessed as dissociation constant incubated for 2 hrs by flow cytometry	Homo sapiens	2	ALA5236624	cell-based format	Scientific Literature
13.	ALA5239852	B	Binding affinity to saturated human muscarinic M3 expressed in CHO cells assessed as dissociation constant incubated for 2 hrs in presence of atropine by flow cytometry	Homo sapiens	2	ALA5236624	cell-based format	Scientific Literature
14.	ALA5239853	B	Binding affinity to saturated human muscarinic M1 expressed in CHO cells assessed as dissociation constant incubated for 2 hrs in presence of atropine by flow cytometry	Homo sapiens	2	ALA5236624	cell-based format	Scientific Literature
15.	ALA5239854	B	Binding affinity to saturated human muscarinic M1 expressed in CHO cells assessed as dissociation constant incubated for 2 hrs by flow cytometry	Homo sapiens	2	ALA5236624	cell-based format	Scientific Literature
16.	ALA5239856	B	Binding affinity to human muscarinic M3 expressed in CHO cells assessed as association rate constant incubated for 0.5 to 36 mins in presence of atropine by flow cytometry	Homo sapiens	1	ALA5236624	cell-based format	Scientific Literature
17.	ALA5239858	B	Binding affinity to human muscarinic M3 expressed in CHO cells assessed as dissociation rate constant preincubated for 20 mins in dark followed by atropine addition and measured after 1 to 130 mins by flow cytometry	Homo sapiens	1	ALA5236624	cell-based format	Scientific Literature
18.	ALA5239859	T	Cytotoxicity against Chinese hamster CHO-K1 cells assessed as reduction in cell viability upto 1 uM by crystal violet staining based multiplate reader analysis	Cricetulus griseus	2	ALA5236624	cell-based format	Scientific Literature
19.	ALA5239860	B	Displacement of atropine from human muscarinic M3 receptor expressed in CHO cells incubated for 2 hrs by flow cytometric competition binding assay	Homo sapiens	2	ALA5236624	cell-based format	Scientific Literature
20.	ALA5239861	B	Displacement of tiotropium bromide from human muscarinic M3 receptor expressed in CHO cells incubated for 4 hrs by flow cytometric competition binding assay	Homo sapiens	2	ALA5236624	cell-based format	Scientific Literature