# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5251899 | B | Inhibition of N-terminal His-tagged recombinant human BTK (393 to 659 residues) expressed in baculovirus infected Sf9 cells using Biotin-AVLESEEELYSSARQ-NH2 as substrate preincubated for 1 hrs followed by substrate addition in presence of ATP at Km concentration and measured after 1 hrs by TR-FRET assay | Homo sapiens | 39 | ALA5247657 | cell-based format | Scientific Literature | |
2. | ALA5251900 | B | Inhibition of EGFR (unknown origin) preincubated for 1 hrs followed by substrate addition and measured after 1 hrs in the presence of ATP at Km concentration by TR-FRET assay | Homo sapiens | 29 | ALA5247657 | single protein format | Scientific Literature | |
3. | ALA5251901 | B | Inhibition of TEC (unknown origin) preincubated for 1 hrs followed by substrate addition and measured after 1 hrs in the presence of ATP at Km concentration by TR-FRET assay | Homo sapiens | 29 | ALA5247657 | single protein format | Scientific Literature | |
4. | ALA5251903 | B | Selectivity ratio of IC50 for inhibition of EGFR (unknown origin) to IC50 for inhibition of N-terminal His-tagged recombinant human BTK (393 to 659 residues) expressed in baculovirus infected Sf9 cells | Homo sapiens | 6 | ALA5247657 | cell-based format | Scientific Literature | |
5. | ALA5251904 | B | Selectivity ratio of IC50 for inhibition of TEC (unknown origin) to IC50 for inhibition of N-terminal His-tagged recombinant human BTK (393 to 659 residues) expressed in baculovirus infected Sf9 cells | Homo sapiens | 8 | ALA5247657 | cell-based format | Scientific Literature | |
6. | ALA5251905 | B | Inhibition of BTK phosphorylation at Tyr 223 residue in human Ramos cell incubated for 3 hrs by HTRF-based analysis | Homo sapiens | 30 | ALA5247657 | cell-based format | Scientific Literature | |
7. | ALA5251907 | F | Antiproliferative activity against human REC1 cells assessed as reduction in cell viability incubated for 6 days by CellTiter-Glo luminescent cell viability assay | Homo sapiens | 6 | ALA5247657 | cell-based format | Scientific Literature | |
8. | ALA5251928 | B | Inhibition of EGFR autophosphorylation at Tyr1068 residue in EGFR-amplified human A-431 cells preincubated with compound for 1 hrs followed by stimulation with human recombinant EGF and measured after 10 mins by HTRF-based analysis | Homo sapiens | 10 | ALA5247657 | cell-based format | Scientific Literature | |
9. | ALA5251929 | B | Inhibition of FLAG-tagged TEC autophosphorylation in HEK293 cells incubated for 2 hrs by MSD electrochemiluminescence immunoassay | Homo sapiens | 10 | ALA5247657 | cell-based format | Scientific Literature | |
10. | ALA5251930 | B | Selectivity ratio of IC50 for inhibition of EGFR autophosphorylation at Tyr1068 residue in EGFR-amplified human A-431 cells to IC50 for inhibition of BTK phosphorylation at Tyr 223 residue in human Ramos cells | Homo sapiens | 10 | ALA5247657 | cell-based format | Scientific Literature | |
11. | ALA5251931 | B | Selectivity ratio of IC50 for inhibition of FLAG-tagged TEC autophosphorylation in HEK293 cells to IC50 for inhibition of BTK phosphorylation at Tyr 223 residue in human Ramos cells | Homo sapiens | 10 | ALA5247657 | cell-based format | Scientific Literature |