# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA5240351 | B | Binding affinity to HIF-2alpha/ARNT (unknown origin) assessed as change in melting temperature at 10 uM by protein thermal shift assay | Homo sapiens | 34 | ALA5236640 | protein format | Scientific Literature | |
2. | ALA5240352 | B | Agonist activity at human HIF-2alpha expressed in human 786-0 cells expressing HRE luciferase reporter gene assessed as increase of HIF stabilization at 20 uM incubated for 24 hrs by Steady-lumi luciferase assay | Homo sapiens | 27 | ALA5236640 | cell-based format | Scientific Literature | |
3. | ALA5240353 | B | Agonist activity at human HIF-2alpha expressed in human 786-0 cells assessed as increase in NDRG1 transcription level at 20 uM incubated for 24 hrs by RT-PCR analysis relative to control | Homo sapiens | 10 | ALA5236640 | cell-based format | Scientific Literature | |
4. | ALA5240354 | B | Agonist activity at human HIF-2alpha expressed in human 786-0 cells assessed as increase in VEGFA transcription level at 20 uM incubated for 24 hrs by RT-PCR analysis relative to control | Homo sapiens | 10 | ALA5236640 | cell-based format | Scientific Literature | |
5. | ALA5240355 | B | Agonist activity at human HIF-2alpha expressed in human 786-0 cells assessed as increase in GLUT1 transcription level at 20 uM incubated for 24 hrs by RT-PCR analysis relative to control | Homo sapiens | 10 | ALA5236640 | cell-based format | Scientific Literature | |
6. | ALA5240356 | B | Agonist activity at human HIF-2alpha expressed in human 786-0 cells assessed as increase in PGK1 transcription level at 20 uM incubated for 24 hrs by RT-PCR analysis relative to control | Homo sapiens | 10 | ALA5236640 | cell-based format | Scientific Literature | |
7. | ALA5240357 | B | Agonist activity at human HIF-2alpha expressed in human 786-0 cells assessed as increase in NDRG1 transcription level at 2 uM incubated for 24 hrs by RT-PCR analysis relative to control | Homo sapiens | 1 | ALA5236640 | cell-based format | Scientific Literature | |
8. | ALA5240358 | B | Agonist activity at human HIF-2alpha expressed in human 786-0 cells assessed as increase in NDRG1 transcription level at 0.2 uM incubated for 24 hrs by RT-PCR analysis relative to control | Homo sapiens | 1 | ALA5236640 | cell-based format | Scientific Literature | |
9. | ALA5240359 | B | Agonist activity at human HIF-2alpha expressed in human 786-0 cells assessed as increase in VEGFA transcription level at 2 uM incubated for 24 hrs by RT-PCR analysis relative to control | Homo sapiens | 1 | ALA5236640 | cell-based format | Scientific Literature | |
10. | ALA5240360 | B | Agonist activity at human HIF-2alpha expressed in human 786-0 cells assessed as increase in VEGFA transcription level at 0.2 uM incubated for 24 hrs by RT-PCR analysis relative to control | Homo sapiens | 1 | ALA5236640 | cell-based format | Scientific Literature | |
11. | ALA5240361 | B | Synergistic agonist activity at human HIF-2alpha expressed in human Hep3B cells assessed as induction of EPO transcription at 5 uM pretreated with FG4592 followed by compound addition and measured after 12 hrs by qRT-PCR analysis | Homo sapiens | 16 | ALA5236640 | cell-based format | Scientific Literature |