#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5251998	B	Displacement of [3H](+)-pentazocine from sigma 1 receptor in guinea pig brain membrane assessed as inhibition constant incubated for 120 mins	Cavia porcellus	12		cell membrane format	Scientific Literature
2.	ALA5251999	B	Displacement of [3H]DTG from sigma 2 receptor in Wistar Han rat liver membrane assessed as inhibition constant incubated for 120 mins	Rattus norvegicus	11		cell membrane format	Scientific Literature
3.	ALA5252001	A	Drug accumulation in human MCF7 cells assessed as increase in mean fluorescence intensity pretreated with (+)-pentazocine for 60 mins followed by compound addition incubated for 45 mins by flow cytometric analysis	Homo sapiens	2		cell-based format	Scientific Literature
4.	ALA5252002	A	Drug accumulation in human MCF7 TMEM97 knock out cells assessed as increase in mean fluorescence intensity pretreated with (+)-pentazocine for 60 mins followed by compound addition incubated for 45 mins by flow cytometric analysis	Homo sapiens	1		cell-based format	Scientific Literature
5.	ALA5252003	B	Displacement of (+)-pentazocine from sigma 1 receptor in human MCF7 cells assessed as dissociation constant in presence of DTG incubated for 120 mins by saturation binding assay	Homo sapiens	2		cell-based format	Scientific Literature
6.	ALA5252004	B	Displacement of (+)-pentazocine from sigma 1 receptor in human MCF7 cells assessed as dissociation constant in presence of 2-(3-(6,7-dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl)propyl)-5-methoxy-3,4-dihydroisoquinolin-1(2H)-one incubated for 120 mins by saturation binding assay	Homo sapiens	2		cell-based format	Scientific Literature
7.	ALA5252005	B	Binding affinity to sigma 2 receptor in human MCF7 cells assessed as increase in fluorescent intensity pretreated with (+)-pentazocine for 60 mins followed by compound addition incubated for 45 mins by flow cytometric analysis	Homo sapiens	1		cell-based format	Scientific Literature
8.	ALA5252006	A	Drug uptake in human MCF7 cells expressing sigma 1 receptor assessed as increase in fluorescence intensity in presence of (+)-pentazocine incubated at 1 to 10000 nM for 45 mins by flow cytometric analysis	Homo sapiens	1		cell-based format	Scientific Literature
9.	ALA5252007	B	Binding affinity to sigma 2 receptor in human MCF7 cells assessed as displacement of compound by measuring inhibition constant of DTG incubated for 120 mins by saturation binding assay	Homo sapiens	2		cell-based format	Scientific Literature
10.	ALA5252008	B	Binding affinity to sigma 2 receptor in human MCF7 cells assessed as displacement of compound by measuring inhibition constant of 2-(3-(6,7-dimethoxy-3,4-dihydroisoquinolin-2(1H)-yl)propyl)-5-methoxy-3,4-dihydroisoquinolin-1(2H)-one incubated for 120 mins by saturation binding assay	Homo sapiens	2		cell-based format	Scientific Literature
11.	ALA5252009	A	Drug localization in human MCF7 cells assessed as visualization of sigma 2 receptor around the nucleus preincubated with (+)-pentazocine for 2 hrs followed by compound addition at 5 uM for 1 hr by trypan blue based confocal microscopic study	Homo sapiens	2		cell-based format	Scientific Literature
12.	ALA5252010	A	Drug localization in mitochondria of human MCF7 cells assessed as increase in drug uptake preincubated with 1-[2-(4-chlorophenoxy)ethyl]-4-methylpiperidine for 75 mins followed by compound addition at 0.05 uM for 30 mins by mito tracker biodipy-FL staining based analysis	Homo sapiens	2		mitochondrion format	Scientific Literature