#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA5260382	B	Ratio of Km for ThDP substrate activity at Zymomonas mobilis pyruvate decarboxylase to Ki for inhibition of Zymomonas mobilis pyruvate decarboxylase assessed as inhibition constant preincubated for 60 mins followed by pyruvate addition using 10 uM ThDP as substrate by microplate reader analysis	Zymomonas mobilis	2	ALA5257137	assay format	Scientific Literature
2.	ALA5260383	B	Inhibition of porcine PDHc E1-subunit assessed as DCPIP reduction at 250 uM preincubated for 30 mins followed by pyruvate addition using 50 uM ThDP as substrate by microplate reader analysis	Sus scrofa	32	ALA5257137	single protein format	Scientific Literature
3.	ALA5260384	B	Inhibition of Escherichia coli OGDHc E1 subunit assessed as DCPIP reduction at 250 uM preincubated for 60 mins followed by pyruvate addition using 50 uM ThDP as substrate by microplate reader analysis	Escherichia coli	32	ALA5257137	assay format	Scientific Literature
4.	ALA5260385	B	Inhibition of Saccharomyces cerevisiae pyruvate decarboxylase assessed as DCPIP reduction at 1.5 mM preincubated for 60 mins followed by pyruvate addition using 0.3 mM ThDP as substrate by microplate reader analysis	Saccharomyces cerevisiae	16	ALA5257137	assay format	Scientific Literature
5.	ALA5260386	B	Inhibition of Aerococcus viridans pyruvate oxidase at 250 uM preincubated for 30 mins followed by pyruvate addition using 50 uM ThDP as substrate by microplate reader analysis	Aerococcus viridans	32	ALA5257137	assay format	Scientific Literature
6.	ALA5260390	B	Inhibition of porcine PDHc E1-subunit assessed as DCPIP reduction preincubated for 30 mins followed by pyruvate addition using 10 uM ThDP as substrate by microplate reader analysis	Sus scrofa	3	ALA5257137	single protein format	Scientific Literature
7.	ALA5260391	B	Inhibition of Zymomonas mobilis pyruvate decarboxylase assessed as reduction in NADH consumption preincubated for 60 mins followed by pyruvate addition using 10 uM ThDP as substrate by microplate reader analysis	Zymomonas mobilis	2	ALA5257137	assay format	Scientific Literature
8.	ALA5260392	B	Inhibition of Escherichia coli OGDHc E1 subunit assessed as DCPIP reduction preincubated for 60 mins followed by alpha-ketoglutarate addition using 30 uM ThDP as substrate by microplate reader analysis	Escherichia coli	2	ALA5257137	assay format	Scientific Literature
9.	ALA5260393	B	Inhibition of Aerococcus viridans pyruvate oxidase preincubated for 30 mins followed by pyruvate addition using 50 uM ThDP as substrate by microplate reader analysis	Aerococcus viridans	2	ALA5257137	assay format	Scientific Literature
10.	ALA5260394	B	Inhibition of porcine PDHc E1-subunit assessed as inhibition constant preincubated for 30 mins followed by pyruvate addition using 10 uM ThDP as substrate by microplate reader analysis	Sus scrofa	3	ALA5257137	single protein format	Scientific Literature
11.	ALA5260395	B	Inhibition of Zymomonas mobilis pyruvate decarboxylase assessed as inhibition constant preincubated for 60 mins followed by pyruvate addition using 10 uM ThDP as substrate by microplate reader analysis	Zymomonas mobilis	2	ALA5257137	assay format	Scientific Literature
12.	ALA5260396	B	Inhibition of Escherichia coli OGDHc E1 subunit assessed as inhibition constant preincubated for 60 mins followed by alpha-ketoglutarate addition using 30 uM ThDP as substrate by microplate reader analysis	Escherichia coli	2	ALA5257137	assay format	Scientific Literature
13.	ALA5260397	B	Inhibition of Aerococcus viridans pyruvate oxidase assessed as inhibition constant preincubated for 30 mins followed by pyruvate addition using 50 uM ThDP as substrate by microplate reader analysis	Aerococcus viridans	2	ALA5257137	assay format	Scientific Literature
14.	ALA5260398	B	Ratio of Km for ThDP substrate activity at porcine PDHc E1-subunit to Ki for inhibition of porcine PDHc E1-subunit assessed as inhibition constant preincubated for 30 mins followed by pyruvate addition using 10 uM ThDP as substrate by microplate reader analysis	Sus scrofa	3	ALA5257137	single protein format	Scientific Literature
15.	ALA5260399	B	Ratio of Km for ThDP substrate activity at Escherichia coli OGDHc E1 to Ki for inhibition of Escherichia coli OGDHc E1 subunit assessed as inhibition constant preincubated for 60 mins followed by alpha-ketoglutarate addition using 30 uM ThDP as substrate by microplate reader analysis	Escherichia coli	2	ALA5257137	assay format	Scientific Literature
16.	ALA5260400	B	Ratio of Km for ThDP substrate activity at Aerococcus viridans pyruvate oxidase to Ki for inhibition of Aerococcus viridans pyruvate oxidase assessed as inhibition constant preincubated for 30 mins followed by pyruvate addition using 50 uM ThDP as substrate by microplate reader analysis	Aerococcus viridans	2	ALA5257137	assay format	Scientific Literature
17.	ALA5260401	F	Antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum 3D7 infected in human erythrocyte assessed as inhibition of parasite proliferation in thiamine free medium by SYBR-Safe assay	Plasmodium falciparum	9	ALA5257137	organism-based format	Scientific Literature
18.	ALA5260402	F	Antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum 3D7 infected in human erythrocyte assessed as inhibition of parasite proliferation in presence of 2.97uM thiamine by SYBR-Safe assay	Plasmodium falciparum	9	ALA5257137	organism-based format	Scientific Literature
19.	ALA5260403	F	Antiplasmodial activity against chloroquine-sensitive Plasmodium falciparum 3D7 infected in human erythrocyte assessed as inhibition of parasite proliferation in presence of 297uM thiamine by SYBR-Safe assay	Plasmodium falciparum	9	ALA5257137	organism-based format	Scientific Literature
20.	ALA5260404	T	Cytotoxicity against human HFF cells assessed as inhibition of cell proliferation incubated for 96 hrs by SYBR- Safe assay	Homo sapiens	9	ALA5257137	cell-based format	Scientific Literature