Inhibition of GCase in human SH-SY5Y cells using MUG-Gluc as fluorogenic substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs in presence of conduritol B epoxide by fluorescence based assay
Inhibition of GCase in human SH-SY5Y cells at 1 nM to 1 mM using MUG-Gluc as fluorogenic substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs in presence of conduritol B epoxide by fluorescence based assay relative to control
Inhibition of NLGase in human SH-SY5Y cells using MUG-Gluc as fluorogenic substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs in presence of AMP-DNM by fluorescence based assay
Inhibition of NLGase in human SH-SY5Y cells at 1 nM to 1 mM using MUG-Gluc as fluorogenic substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs in presence of AMP-DNM by fluorescence based assay relative to control
Anti-inflammatory activity in human CuFi1 cells assessed as reduction in Pseudomonas aeruginosa PAO1-induced IL-8 mRNA expression level at 0.1 uM pretreated for 1 hr followed by Pseudomonas aeruginosa PAO1 challenge and measured after 4 hrs by RT-PCR analysis relative to control
Anti-inflammatory activity in human CuFi1 cells assessed as reduction in Pseudomonas aeruginosa PAO1-induced IL-8 mRNA expression level pretreated for 1 hr followed by Pseudomonas aeruginosa PAO1 challenge and measured after 4 hrs by RT-PCR analysis
Anti-inflammatory activity in human CuFi1 cells assessed as reduction in Pseudomonas aeruginosa PAO1-induced IL-8 mRNA expression level pretreated for 1 hr followed by Pseudomonas aeruginosa PAO1 challenge and measured after 4 hrs by RT-PCR analysis relative to control
Anti-inflammatory activity in human Bronchial epithelial cells assessed as reduction in Pseudomonas aeruginosa PAO1-induced IL-8 mRNA expression level at 10 nM pretreated for 1 hr followed by Pseudomonas aeruginosa PAO1 challenge and measured after 4 hrs by RT-PCR analysis relative to control
Anti-inflammatory activity in Pseudomonas aeruginosa PAO1 infected C57Bl/6NCr mouse assessed as reduction in amount of neutrophils in lungs at 100 mg/kg, po administered via gavage pretreated for 1 to 24 hrs followed by Pseudomonas aeruginosa PAO1 challenge and measured after 6 hrs
Anti-inflammatory activity in Pseudomonas aeruginosa PAO1 infected C57Bl/6NCr mouse assessed as increase in alveolar macrophages in lungs at 10 mg/kg, po administered via gavage pretreated for 1 to 24 hrs followed by Pseudomonas aeruginosa PAO1 challenge and measured after 6 hrs
Anti-inflammatory activity in Pseudomonas aeruginosa PAO1 infected C57Bl/6NCr mouse assessed as reduction in bacterial CFU in BAL at 10 mg/kg, po administered via gavage pretreated for 1 to 24 hrs followed by Pseudomonas aeruginosa PAO1 challenge and measured after 6 hrs
Anti-inflammatory activity in Pseudomonas aeruginosa PAO1 infected C57Bl/6NCr mouse assessed as reduction in bacterial CFU in lungs at 10 mg/kg, po administered via gavage pretreated for 1 to 24 hrs followed by Pseudomonas aeruginosa PAO1 challenge and measured after 6 hrs
Toxicity in Pseudomonas aeruginosa PAO1 infected C57Bl/6NCr mouse assessed as change in body weight at 100 mg/kg, po administered via gavage pretreated for 1 to 24 hrs followed by Pseudomonas aeruginosa PAO1 challenge and measured after 6 hrs