#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3706186	Binding	In Vitro HPLC Assay: This assay was used to determine the inhibiting effect of the inhibitors in a concentration of 1 mM. A standard incubation mixture consisted of 75 μl of 50 mM potassium phosphate buffer pH 7.4 and contained NMMA in a concentration of 200 μM. The inhibitors were used in final concentrations of 1 mM. By adding 2 μg of hDDAH-1, the reaction was started and the samples were incubated for 30 minutes at 37° C. in, a shaking water bath. The reaction was then stopped by adding 75 μl of acetonitrile, the samples were shaken for 5′ minutes and centrifuged (12,000 g, 5 min). The supernatant was added to the HPLC.	Homo sapiens	2	ALA3638526	single protein format	BindingDB Database
2.	ALA832369	Binding	Inhibition of [14C]-L-NMMA (1 umol/L) binding to dimethylarginine dimethylaminohydrolase of rat kidney homogenate		14	ALA1145158	assay format	Scientific Literature
3.	ALA834957	Binding	Inhibition of [14C]-L-NMMA (1 umol/L) binding to dimethylarginine dimethylaminohydrolase of rat kidney homogenate 100 uM	Rattus norvegicus	8	ALA1145158	assay format	Scientific Literature
4.	ALA1020493	Binding	Inhibition of human DDAH1 at 1 mM by HPLC	Homo sapiens	14	ALA1139258	single protein format	Scientific Literature
5.	ALA1020494	Binding	Inhibition of human DDAH1 by plate-reader assay	Homo sapiens	9	ALA1139258	single protein format	Scientific Literature
6.	ALA1020496	Binding	Inhibition of rat DDAH	Rattus norvegicus	1	ALA1139258	single protein format	Scientific Literature
7.	ALA1020497	Binding	Inhibition of human recombinant nNOS at 100 uM	Homo sapiens	14	ALA1139258	single protein format	Scientific Literature
8.	ALA1020498	Binding	Inhibition of human recombinant eNOS at 100 uM	Homo sapiens	14	ALA1139258	single protein format	Scientific Literature
9.	ALA1020499	Binding	Inhibition of human recombinant iNOS at 1 uM	Homo sapiens	14	ALA1139258	single protein format	Scientific Literature
10.	ALA1020500	Binding	Inhibition of human recombinant nNOS at 1 uM	Homo sapiens	14	ALA1139258	single protein format	Scientific Literature
11.	ALA1020501	Binding	Inhibition of human recombinant eNOS at 1 uM	Homo sapiens	14	ALA1139258	single protein format	Scientific Literature
12.	ALA1020502	Binding	Inhibition of human recombinant iNOS at 100 uM	Homo sapiens	14	ALA1139258	single protein format	Scientific Literature
13.	ALA3883167	Binding	Inhibition of DDAH in HMEC1 assessed as increase in intracellular ADMA levels at 20 uM after 24 hrs by ELISA relative to vehicle-treated control	Homo sapiens	3	ALA3882738	cell-based format	Patent Bioactivity Data
14.	ALA3883173	Binding	Inhibition of DDAH in lung fibroblasts isolated from late stage idiopathic pulmonary fibrosis patient assessed as reduction of TGF-beta-induced acid-soluble collagen levels at 50 uM after 24 hrs by sircol collagen assay	Homo sapiens	2	ALA3882738	single protein format	Patent Bioactivity Data
15.	ALA3883178	Binding	Inhibition of DDAH in human A549 cells assessed as decrease in serum-stimulated cell proliferation at 50 to 100 uM preincubated for 2 hrs followed by fetal bovine serum addition measured 24 hrs post compound treatment by BrdU incorporation assay	Homo sapiens	1	ALA3882738	cell-based format	Patent Bioactivity Data
16.	ALA3883179	Binding	Inhibition of DDAH in mouse MLE12 cells assessed as decrease in serum-stimulated cell proliferation at 50 to 100 uM preincubated for 2 hrs followed by fetal bovine serum addition measured 24 hrs post compound treatment by BrdU incorporation assay	Mus musculus	1	ALA3882738	cell-based format	Patent Bioactivity Data
17.	ALA4380142	Binding	Inhibition of 6xHis-tagged recombinant human DDAH1 expressed in Escherichia coli BL21 cells using NMMA as substrate measured after 30 mins by colder reagent based microplate reader assay	Homo sapiens	18	ALA4376856	cell-based format	Scientific Literature
18.	ALA4380143	Binding	Inhibition of bovine liver arginase at 1 mM using L-arginine as substrate measured after 30 mins by colder reagent based microplate reader assay relative to control	Bos taurus	16	ALA4376856	assay format	Scientific Literature
19.	ALA4380144	Binding	Inhibition of neuronal NOS (unknown origin) assessed as formation of methemoglobin from oxyhemoglobin at 1 mM using L-arginine as substrate in presence of oxyhemoglobin and NADPH by UV-vis spectrophotometry relative to control	Homo sapiens	16	ALA4376856	single protein format	Scientific Literature
20.	ALA4380145	Binding	Inhibition of endothelial NOS (unknown origin) assessed as formation of methemoglobin from oxyhemoglobin at 1 mM using L-arginine as substrate in presence of oxyhemoglobin and NADPH by UV-vis spectrophotometry relative to control	Homo sapiens	16	ALA4376856	single protein format	Scientific Literature