| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA893657 | F | Decrease in androgen receptor protein expression in LNCaP cells by Western blotting | Homo sapiens | 11 | cell-based format | Scientific Literature | ||
| 2. | ALA893658 | F | Growth inhibition of human LNCaP cells after 3 days by MTT colorimetric assay | Homo sapiens | 6 | cell-based format | Scientific Literature | ||
| 3. | ALA4670803 | B | Inhibition of TEAD4 in HEK293 cells assessed as reduction in ANKRD1 mRNA expression at 1 uM measured after 6 hrs by SYBR green dye based RT-PCR analysis relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 4. | ALA4670804 | B | Binding affinity to N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells assessed as inhibition of protein-FAM-YAP (50 to 100 residues) interaction preincubated for 10 mins followed by FAM YAP addition and measured after 1 hr by fluorescence assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA4670806 | B | Binding affinity to nonacylated mouse TEAD4 assessed as line broadening at Phe408 residue at 0.5 mM by 1H-15N HSQC spectra analysis | Mus musculus | 1 | single protein format | Scientific Literature | ||
| 6. | ALA4670807 | B | Binding affinity to nonacylated mouse TEAD4 assessed as line broadening at Phe386 residue at 0.5 mM by 1H-15N HSQC spectra analysis | Mus musculus | 1 | single protein format | Scientific Literature | ||
| 7. | ALA4670808 | B | Binding affinity to TEAD4 in human HCT-116 cells assessed as change in melting temperature at 50 uM after 10 mins by CESTA | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA4670809 | B | Binding affinity to N-terminal His6/MBP-tagged mouse TEAD4 (209 to 427 residues) Cys360Ala mutant expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells assessed as inhibition of protein-FAM-YAP (50 to 100 residues) interaction preincubated for 10 mins followed by FAM YAP addition and measured after 1 hr by fluorescence assay | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4670810 | B | Binding affinity to nonacylated N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells assessed as inhibition of protein-FAM-YAP (50 to 100 residues) interaction preincubated for 10 mins followed by FAM YAP addition and measured after 1 hr by fluorescence assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 10. | ALA4670811 | B | Binding affinity to N-terminal His6-tagged mouse TEAD4 Cys360Ser mutant assessed as inhibition of protein-FAM-YAP (50 to 100 residues) interaction preincubated for 10 mins followed by FAM YAP addition and measured after 1 hr by fluorescence assay | Mus musculus | 1 | assay format | Scientific Literature | ||
| 11. | ALA4670813 | B | Inhibition of CPM binding to N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells preincubated for 10 mins followed by CPM addition and measured after 1 hr by fluorescence assay | Homo sapiens | 38 | cell-based format | Scientific Literature | ||
| 12. | ALA4670814 | B | Inhibition of CPM binding to N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells at 12.5 uM preincubated for 10 mins followed by CPM addition and measured after 1 hr by fluorescence assay relative to control | Homo sapiens | 34 | cell-based format | Scientific Literature | ||
| 13. | ALA4670815 | B | Inhibition of FITC-labeled palmitate tracer binding to N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells preincubated for 10 mins followed by FITC-labeled palmitate tracer addition and measured after 1 hr by fluorescence assay | Homo sapiens | 31 | cell-based format | Scientific Literature | ||
| 14. | ALA4670817 | B | Binding affinity to nonacylated N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells assessed as melting temperature at 80 uM after 10 mins by SYPRO orange dye-based differential scanning fluorimetry method (Rvb = 41 degreeC) | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
| 15. | ALA4670818 | B | Binding affinity to nonacylated N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells at 8 equiv after 1 hr by ESI-MS spectrophotometric analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 16. | ALA4670819 | B | Binding affinity to acylated N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells assessed as covalent adduct formation at 8 equiv after 1 hr by ESI-MS spectrophotometric analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA4670820 | B | Inhibition of CPM binding to N-terminal His6-tagged mouse TEAD4 Cys360Ser mutant at 10 uM preincubated for 10 mins followed by CPM addition and measured after 1 hr by fluorescence assay | Mus musculus | 1 | assay format | Scientific Literature | ||
| 18. | ALA4670821 | B | Inhibition of CPM binding to N-terminal His6/MBP-tagged mouse TEAD4 (209 to 427 residues) Cys360Ala mutant expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells at 10 uM preincubated for 10 mins followed by CPM addition and measured after 1 hr by fluorescence assay | Mus musculus | 1 | cell-based format | Scientific Literature | ||
| 19. | ALA4670822 | B | Reversible inhibition of CPM binding to nonacylated N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells measured after 1 to 24 hrs by fluorescence assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 20. | ALA4670823 | B | Inhibition of CPM binding to nonacylated N-terminal His6-tagged human TEAD4 (217 to 434 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells assessed as ratio of Kinact to Ki measured after 1 to 24 hrs by fluorescence assay | Homo sapiens | 1 | cell-based format | Scientific Literature |
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