#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA925866	B	Binding affinity to eukaryotic translation initiation factor 4E		1	ALA1142223	single protein format	Scientific Literature
2.	ALA3295044	B	Displacement of N-alpha-fluorescein tagged eIF4G-derived peptide from GST-DN26-eIF4E (unknown origin) by fluorescence polarization assay	Homo sapiens	27	ALA3286149	single protein format	Scientific Literature
3.	ALA3295045	F	Antiproliferative activity against human CRL2813 cells assessed as inhibition of cell proliferation after 5 days by sulforhodamine B assay	Homo sapiens	27	ALA3286149	cell-based format	Scientific Literature
4.	ALA3295046	F	Antiproliferative activity against human CRL2351 cells assessed as inhibition of cell proliferation after 5 days by sulforhodamine B assay	Homo sapiens	27	ALA3286149	cell-based format	Scientific Literature
5.	ALA3378730	P	Retention time of the compound in 50 to 100% ACN/Water/0.1%TFA by RP-HPLC analysis		34	ALA3351917	small-molecule physicochemical format	Scientific Literature
6.	ALA3378733	B	Competitive binding affinity to human full length recombinant GST-tagged eIF4E expressed in Escherichia coli BL21(DE3) assessed as inhibition of eIF4E/eIF4G interaction by fluorescence polarization assay relative to parent compound	Homo sapiens	54	ALA3351917	assay format	Scientific Literature
7.	ALA3378734	B	Binding affinity to human full length recombinant GST-tagged eIF4E expressed in Escherichia coli BL21(DE3) by intrinsic tryptophan fluorescence quenching method	Homo sapiens	3	ALA3351917	assay format	Scientific Literature
8.	ALA3378735	B	Inhibition of eIF4E/eIF4G interaction in human CRL2813 cells assessed as downregulation of cyclin D1 protein expression at 15 to 30 uM after 6 hrs by Western blotting analysis	Homo sapiens	3	ALA3351917	cell-based format	Scientific Literature
9.	ALA3378736	B	Inhibition of eIF4E/eIF4G interaction in human CRL2813 cells assessed as downregulation of cyclin E protein expression at 15 to 30 uM after 6 hrs by Western blotting analysis	Homo sapiens	3	ALA3351917	cell-based format	Scientific Literature
10.	ALA3378737	B	Inhibition of eIF4E/eIF4G interaction in human CRL2813 cells assessed as downregulation of survivin protein expression at 15 to 30 uM after 6 hrs by Western blotting analysis	Homo sapiens	3	ALA3351917	cell-based format	Scientific Literature
11.	ALA3378738	B	Inhibition of eIF4E/eIF4G interaction in human CRL2813 cells assessed as downregulation of cyclin D1 mRNA expression at 30 uM after 6 hrs by RT-PCR analysis	Homo sapiens	3	ALA3351917	cell-based format	Scientific Literature
12.	ALA3378739	B	Inhibition of eIF4E/eIF4G interaction in human CRL2813 cells assessed as downregulation of cyclin E mRNA expression at 30 uM after 6 hrs by RT-PCR analysis	Homo sapiens	3	ALA3351917	cell-based format	Scientific Literature
13.	ALA3378740	B	Inhibition of eIF4E/eIF4G interaction in human CRL2813 cells assessed as downregulation of survivin mRNA expression at 30 uM after 6 hrs by RT-PCR analysis	Homo sapiens	3	ALA3351917	cell-based format	Scientific Literature
14.	ALA4219797	F	Antiproliferative activity against human BJAB cells after 4 days	Homo sapiens	50	ALA4219038	cell-based format	Scientific Literature
15.	ALA4303083	F	IC50 for antiviral activity against SARS-CoV-2 in the Vero E6 cell line at 48 h by immunofluorescence-based assay (detecting the viral NP protein in the nucleus of the Vero E6 cells).	Chlorocebus sabaeus	57	ALA4303773	organism-based format	SARS-CoV-2 Screening Data 2020-21
16.	ALA4303085	F	AUC (viral infection %) for SARS-CoV-2 in the Vero E6 cell line at 48 h by immunofluorescence-based assay (detecting the viral NP protein in the nucleus of the Vero E6 cells).	Chlorocebus sabaeus	57	ALA4303773	organism-based format	SARS-CoV-2 Screening Data 2020-21
17.	ALA4303086	T	Cytotoxicity of compound against Vero E6 cells by MTT assay.	Chlorocebus sabaeus	57	ALA4303773	cell-based format	SARS-CoV-2 Screening Data 2020-21
18.	ALA4303089	T	AUC (cytotoxicity %) of compound against Vero E6 cells by MTT assay.	Chlorocebus sabaeus	57	ALA4303773	cell-based format	SARS-CoV-2 Screening Data 2020-21
19.	ALA4303091	T	Selectivity ratio: ratio of AUC (viral infection %) of SARS-CoV-2 in the Vero E6 cell line compared to AUC (cytotoxicity %) of compound against Vero E6 cells by MTT assay.		57	ALA4303773	cell-based format	SARS-CoV-2 Screening Data 2020-21
20.	ALA4303092	F	Overall antiviral activity against SARS-CoV-2 (isolate France/IDF0372/2020) in the Vero E6 cell line at 48 h based on three assays 1) detection of viral RNA by qRT-PCR (targeting the N-gene), 2) plaque assay using lysate 3 days after addition of compound by crystal violet staining 3) Cell viability (cytotoxicity) measuring percentage viable Vero E6 cells 48 h after compound addition by AlamarBlue assay.	Chlorocebus sabaeus	59	ALA4303773	organism-based format	SARS-CoV-2 Screening Data 2020-21