#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA930805	B	Inhibition of human CYP17 expressed in Escherichia coli co-expressed with NADPH-P450 reductase at 0.2 uM	Homo sapiens	31	ALA1141584	assay format	Scientific Literature
2.	ALA930806	B	Inhibition of human CYP17 expressed in Escherichia coli co-expressed with NADPH-P450 reductase at 2 uM	Homo sapiens	31	ALA1141584	assay format	Scientific Literature
3.	ALA1656352	B	Inhibition of human CYP11B1 expressed in hamster V79MZ cells using 11-deoxycorticosterone substrate	Homo sapiens	39	ALA1649094	cell-based format	Scientific Literature
4.	ALA1656353	B	Inhibition of human CYP11B2 expressed in hamster V79MZ cells using 11-deoxycorticosterone substrate	Homo sapiens	39	ALA1649094	cell-based format	Scientific Literature
5.	ALA1656354	B	Selectivity index, ratio of IC50 for human CYP11B2 to IC50 for human CYP11B1	Homo sapiens	32	ALA1649094	assay format	Scientific Literature
6.	ALA1656355	B	Inhibition of human recombinant CYP17 expressed in Escherichia coli at 2 uM using progesterone substrate	Homo sapiens	38	ALA1649094	assay format	Scientific Literature
7.	ALA1656356	B	Inhibition of human placental microsomes CYP19 at 500 nM using androstenedione substrate	Homo sapiens	39	ALA1649094	assay format	Scientific Literature
8.	ALA2015355	B	Inhibition of human CYP11B1 expressed in hamster V79 MZ cells using [3H] 11 deoxycorticosterone as substrate by HPLC radioflow detector	Homo sapiens	46	ALA2010745	cell-based format	Scientific Literature
9.	ALA2015356	B	Inhibition of human CYP11B2 expressed in hamster V79 MZ cells using [3H] 11 deoxycorticosterone as substrate by HPLC radioflow detector	Homo sapiens	44	ALA2010745	cell-based format	Scientific Literature
10.	ALA2015358	B	Selectivity ratio of IC50 for human CYP11B2 to IC50 for human CYP11B1	Homo sapiens	31	ALA2010745	assay format	Scientific Literature
11.	ALA2423497	B	Selectivity factor, ratio of IC50 for human CYP11B2 to IC50 for human CYP11B1	Homo sapiens	31	ALA2417547	assay format	Scientific Literature
12.	ALA2423498	B	Inhibition of human CYP11B2 expressed in hamster V79MZh cells using [1,2-3H]-11-deoxycorticosterone as substrate	Homo sapiens	32	ALA2417547	cell-based format	Scientific Literature
13.	ALA2423499	B	Inhibition of human CYP11B1 expressed in hamster V79MZh cells using [1,2-3H]-11-deoxycorticosterone as substrate	Homo sapiens	32	ALA2417547	cell-based format	Scientific Literature
14.	ALA3424479	B	Inhibition of human CYP11B1 expressed in hamster fibroblast using 100 nM [3H]-11-deoxycorticosterone as substrate after 25 mins by HPLC analysis	Homo sapiens	30	ALA3421536	assay format	Scientific Literature
15.	ALA3424480	B	Inhibition of human CYP11B2 expressed in hamster fibroblast using 100 nM [3H]-11-deoxycorticosterone as substrate after 45 mins by HPLC analysis	Homo sapiens	30	ALA3421536	assay format	Scientific Literature
16.	ALA3424481	B	Inhibition of human CYP19 expressed in hamster fibroblast using 500 nM [1beta-3H] androstenedione as substrate by HPLC analysis	Homo sapiens	16	ALA3421536	assay format	Scientific Literature
17.	ALA3424482	B	Inhibition of rat CYP11B1 expressed in hamster fibroblast using 500 nM [3H]-11-deoxycorticosterone as substrate after 7 hrs by HPLC analysis	Rattus norvegicus	14	ALA3421536	assay format	Scientific Literature
18.	ALA3424483	B	Selectivity index, ratio of IC50 for human CYP11B2 to IC50 for human CYP11B1	Homo sapiens	30	ALA3421536	assay format	Scientific Literature
19.	ALA3888752	B	Cellular Inhibition Assay: V79MZ cells expressing human CYP11B1 and human CYP11B2 genes, respectively, were grown on 24-well cell culture plates (8×10^5 cells per well) with 1.9 cm^2 culture area per well in 1 ml DMEM culture medium until confluence. Before testing, the DMEM culture medium was removed and 450 μl of fresh DMEM, containing the inhibitor in at least three different concentrations for determining the IC50 value, was added to each well. Every value was determined at least three times. After a pre-incubation step of 60 min at 37° C., the reaction was started by the addition of 50 μl of DMEM containing the substrate 11-deoxycorticosterone (containing 0.15 μCi of [1,2-3H] 11-deoxycorticosterone, dissolved in ethanol, final concentration 100 nM). The V79MZh11B1 cells were incubated for 25 min, the V79MZh11B2 cells were incubated for 50 min. Controls were treated in the same way without inhibitors. The maximum DMSO concentration in each well was 1%. Enzyme reactions were stopped by extracting the supernatant with 500 μl ethyl acetate. Samples were centrifuged (10000×g, 2 min), and the solvent was pipetted into fresh cups. The solvent was evaporated and the steroids were redissolved in 40 μl of methanol and analyzed by HPLC using radioflow detection (Ehmer et al. J. Steroid Biochem. Mol. Biol. 2002, 81, 173-179 and Denner et al. Endocr. Res. 1995, 21, 443-448).	Homo sapiens	46	ALA3886821	cell-based format	BindingDB Database
20.	ALA3888753	B	Cellular Inhibition Assay: V79MZ cells expressing human CYP11B1 and human CYP11B2 genes, respectively, were grown on 24-well cell culture plates (8×10^5 cells per well) with 1.9 cm^2 culture area per well in 1 ml DMEM culture medium until confluence. Before testing, the DMEM culture medium was removed and 450 μl of fresh DMEM, containing the inhibitor in at least three different concentrations for determining the IC50 value, was added to each well. Every value was determined at least three times. After a pre-incubation step of 60 min at 37° C., the reaction was started by the addition of 50 μl of DMEM containing the substrate 11-deoxycorticosterone (containing 0.15 μCi of [1,2-3H] 11-deoxycorticosterone, dissolved in ethanol, final concentration 100 nM). The V79MZh11B1 cells were incubated for 25 min, the V79MZh11B2 cells were incubated for 50 min. Controls were treated in the same way without inhibitors. The maximum DMSO concentration in each well was 1%. Enzyme reactions were stopped by extracting the supernatant with 500 μl ethyl acetate. Samples were centrifuged (10000×g, 2 min), and the solvent was pipetted into fresh cups. The solvent was evaporated and the steroids were redissolved in 40 μl of methanol and analyzed by HPLC using radioflow detection (Ehmer et al. J. Steroid Biochem. Mol. Biol. 2002, 81, 173-179 and Denner et al. Endocr. Res. 1995, 21, 443-448).	Homo sapiens	44	ALA3886821	cell-based format	BindingDB Database