# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1029994 | F | Cytotoxicity against mouse LLC cells after 48 hrs by SRB assay | Mus musculus | 5 | cell-based format | Scientific Literature | ||
2. | ALA953970 | F | Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production by measuring nitrite level after 24 hrs | Mus musculus | 7 | cell-based format | Scientific Literature | ||
3. | ALA953971 | F | Cell viability of LPS-stimulated mouse RAW264.7 cells after 24 hrs by MTT assay | Mus musculus | 7 | cell-based format | Scientific Literature | ||
4. | ALA3706222 | B | Protease Assay: The HCV protease assay herein was applied to investigate the HCV-protease inhibitory activity of the prepared compounds as described above. The method of the HCV protease assay was described in D. T. Phuong, C. M. Ma, M. Hattori and J. S. J in: Inhibitory Effects of Antrodins A-E from Antrodia cinnamomea and Their Metabolites on Hepatitis C Virus Protease. Phytotherapy Research, 23, 582-584, 2009. Two micro liters of a compound solution (using DMSO as solvent) was placed in 384 well micro plate, then 8 ul of HCV NS3/4A protease (0.5 g/mL) was added to the well containing a sample and the plate was agitated. Finally, 10 uL of freshly prepared substrate (Ac-Asp-Glu-Dap(QXL 520)-Glu-Glu-Abu-COO-Ala-Ser-Cys(5-FAMsp)-NH2) (100 dilution of a DMSO stock solution) was added with sequential rotational shaking. The reaction mixture was incubated for 30 min at 37 C. The fluorimetric analyses were performed on an automated TECAN GENios plate reader with excitation wavelength at 485 nm. | Hepatitis C virus genotype 1a (isolate H) | 65 | single protein format | BindingDB Database |