#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA963040	B	Initial binding affinity to wild type human PNP	Homo sapiens	10		single protein format	Scientific Literature
2.	ALA963041	B	Equilibrium binding affinity to wild type human PNP	Homo sapiens	10		single protein format	Scientific Literature
3.	ALA963043	B	Ratio of Km for wild type human PNP to Ki for wild type human PNP relative to inosine	Homo sapiens	10		single protein format	Scientific Literature
4.	ALA963045	B	Equilibrium binding affinity to human PNP His257Gly mutant	Homo sapiens	10		assay format	Scientific Literature
5.	ALA963047	B	Ratio of Km for human PNP His257Gly mutant to Ki for human PNP His257Gly mutant relative to inosine	Homo sapiens	10		assay format	Scientific Literature
6.	ALA963048	B	Ratio of relative binding Km/Ki for wild type human PNP to relative binding affinity Km/Ki for human PNP His257Gly mutant	Homo sapiens	10		assay format	Scientific Literature
7.	ALA960618	B	Initial binding affinity to human PNP His257Gly mutant	Homo sapiens	9		assay format	Scientific Literature
8.	ALA3888213	B	Inhibition Assay: Assays for slow-onset inhibitors were carried out by adding 1 nM PaMTIP into reaction mixtures at 25 °C. containing 100 mM Hepes, pH 7.4, 100 mM phosphate, pH 7.4, 2 mM MTI, 5 mM DTT, 0.5 unit of xanthine oxidase and variable inhibitor concentration. Inhibitors were present at >10 times the enzyme concentration. Assays for MTA inhibition used 200 μM MTI. Controls having no enzyme and no inhibitor were included in all of the inhibition assays. Slow-onset inhibitors display a second phase of tighter binding after reaching a thermodynamic equilibrium with the enzyme. The equilibrium constant for the second binding phase is indicated as Ki*.		8		single protein format	BindingDB Database
9.	ALA3888214	B	Inhibition Assay: Assays for slow-onset inhibitors were carried out by adding 1 nM PaMTIP into reaction mixtures at 25 °C. containing 100 mM Hepes, pH 7.4, 100 mM phosphate, pH 7.4, 2 mM MTI, 5 mM DTT, 0.5 unit of xanthine oxidase and variable inhibitor concentration. Inhibitors were present at >10 times the enzyme concentration. Assays for MTA inhibition used 200 μM MTI. Controls having no enzyme and no inhibitor were included in all of the inhibition assays. Ki is the inhibition constant.		7		single protein format	BindingDB Database