Inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as reduction in DHFR steady state activity at 500 uM using [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by NADPH based radiometric chemical quench assay relative to control
Inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as reduction in DHFR steady state activity using [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by NADPH based radiometric chemical quench assay relative to control
Luciferase/luciferin-expressing antifolate-resistant parasites were used to infect a culture of HepG2 cells that were pre-incubated with compounds. Infected hepatocytes emit light due to the luciferase reaction. Assay results are presented as the percent inhibition of light emission compared to control. (Single Point Inhibition, %)
Luciferase/luciferin-expressing antifolate-resistant parasites were used to infect a culture of HepG2 cells that were pre-incubated with compounds. Infected hepatocytes emit light due to the luciferase reaction. Assay results are presented as the percent inhibition of light emission compared to control. (Single Point Inhibition, %)