# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA963040 | B | Initial binding affinity to wild type human PNP | Homo sapiens | 10 | single protein format | Scientific Literature | ||
2. | ALA963041 | B | Equilibrium binding affinity to wild type human PNP | Homo sapiens | 10 | single protein format | Scientific Literature | ||
3. | ALA963043 | B | Ratio of Km for wild type human PNP to Ki for wild type human PNP relative to inosine | Homo sapiens | 10 | single protein format | Scientific Literature | ||
4. | ALA963045 | B | Equilibrium binding affinity to human PNP His257Gly mutant | Homo sapiens | 10 | assay format | Scientific Literature | ||
5. | ALA963047 | B | Ratio of Km for human PNP His257Gly mutant to Ki for human PNP His257Gly mutant relative to inosine | Homo sapiens | 10 | assay format | Scientific Literature | ||
6. | ALA963048 | B | Ratio of relative binding Km/Ki for wild type human PNP to relative binding affinity Km/Ki for human PNP His257Gly mutant | Homo sapiens | 10 | assay format | Scientific Literature | ||
7. | ALA963049 | B | Selectivity for Plasmodium falciparum PNP over human PNP | 2 | assay format | Scientific Literature | |||
8. | ALA960618 | B | Initial binding affinity to human PNP His257Gly mutant | Homo sapiens | 9 | assay format | Scientific Literature | ||
9. | ALA3888213 | B | Inhibition Assay: Assays for slow-onset inhibitors were carried out by adding 1 nM PaMTIP into reaction mixtures at 25 °C. containing 100 mM Hepes, pH 7.4, 100 mM phosphate, pH 7.4, 2 mM MTI, 5 mM DTT, 0.5 unit of xanthine oxidase and variable inhibitor concentration. Inhibitors were present at >10 times the enzyme concentration. Assays for MTA inhibition used 200 μM MTI. Controls having no enzyme and no inhibitor were included in all of the inhibition assays. Slow-onset inhibitors display a second phase of tighter binding after reaching a thermodynamic equilibrium with the enzyme. The equilibrium constant for the second binding phase is indicated as Ki*. | 8 | single protein format | BindingDB Database | |||
10. | ALA4377142 | B | Inhibition of Plasmodium falciparum 3D7 PNP expressed in Escherichia coli assessed as reduction in uric acid formation by spectrophotometric method | Plasmodium falciparum 3D7 | 1 | assay format | Scientific Literature | ||
11. | ALA4377143 | B | Inhibition of human PNP expressed in Escherichia coli assessed as reduction in uric acid formation by spectrophotometric method | Homo sapiens | 1 | assay format | Scientific Literature | ||
12. | ALA4377144 | F | Antimalarial activity against Plasmodium falciparum 3D7 infected in human erythrocytes assessed as reduction in [3H]ethanolamine incorporation incubated for 18 hrs followed by addition of [3H]ethanolamine and measured after 48 hrs by scintillation counting method | Plasmodium falciparum | 2 | organism-based format | Scientific Literature |