# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA972434 | Binding | Binding affinity to SHH | 1 | ALA1138588 | single protein format | Scientific Literature | ||
2. | ALA1101544 | Binding | Inhibition of SHH in mouse Ptch deffective MEF cells after 30 hrs by Gli-responsive beta-galactosidse reporter gene assay | Mus musculus | 18 | ALA1154349 | cell-based format | Scientific Literature | |
3. | ALA1101545 | Functional | Inhibition of SHH in mouse Shh Light2 cells after 30 hrs by GLI-responsive bright glo luciferase reporter gene assay | Mus musculus | 14 | ALA1154349 | cell-based format | Scientific Literature | |
4. | ALA1101547 | Functional | Inhibition of SHH in mouse Shh Light2 cells after 30 hrs by GLI-responsive bright glo luciferase reporter gene assay relative to 6 uM cyclopamine | Mus musculus | 7 | ALA1154349 | cell-based format | Scientific Literature | |
5. | ALA1101549 | ADME | Cytotoxicity against mouse Shh Light2 cells by cell titer glo assay | Mus musculus | 18 | ALA1154349 | cell-based format | Scientific Literature | |
6. | ALA1101550 | Binding | Binding affinity to recombinant Shh N-terminal peptide by surface plasma resonance | 18 | ALA1154349 | single protein format | Scientific Literature | ||
7. | ALA1220906 | Binding | Binding affinity to N-terminal Shh by surface plasmon resonance assay | 2 | ALA1212710 | single protein format | Scientific Literature | ||
8. | ALA1220907 | Functional | Inhibition of N-palmitoylated Shh in mouse Shh Light2 cells after 30 hrs by firefly luciferase reporter gene assay | Mus musculus | 2 | ALA1212710 | cell-based format | Scientific Literature | |
9. | ALA1220908 | ADME | Cytotoxicity against mouse Shh Light2 cells assessed as cell viability | Mus musculus | 2 | ALA1212710 | cell-based format | Scientific Literature | |
10. | ALA1220909 | Functional | Inhibition of N-palmitoylated Shh in mouse Shh Light2 cells at > 30 uM after 30 hrs by firefly luciferase reporter gene assay | Mus musculus | 1 | ALA1212710 | cell-based format | Scientific Literature | |
11. | ALA1220912 | Functional | Inhibition of N-palmitoylated Shh in mouse Shh Light2 cells at 6.3 to 50 uM after 30 hrs by firefly luciferase reporter assay in presence of 3.6 uM Smo agonist purmorphamine | Mus musculus | 1 | ALA1212710 | cell-based format | Scientific Literature | |
12. | ALA1220913 | Functional | Inhibition of N-palmitoylated Shh in mouse Shh Light2 cells at 6.3 to 50 uM after 30 hrs by firefly luciferase reporter assay in presence of 100 nM Smo agonist SAG | Mus musculus | 1 | ALA1212710 | cell-based format | Scientific Literature | |
13. | ALA1220914 | Binding | Inhibition of N-terminal Shh-induced differentiation in mouse C3H10T1/2 cells assessed as inhibition of alkaline phosphatase induction | Mus musculus | 1 | ALA1212710 | cell-based format | Scientific Literature | |
14. | ALA1220915 | Binding | Inhibition of N-terminal Shh-induced differentiation in mouse C3H10T1/2 cells assessed as inhibition of alkaline phosphatase induction in presence of 3.6 uM purmorphamine | Mus musculus | 1 | ALA1212710 | cell-based format | Scientific Literature | |
15. | ALA1220916 | Binding | Displacement of BODIPY-cyclopamine from Smo expressed in HEK293T cells after 30 mins | 1 | ALA1212710 | cell-based format | Scientific Literature | ||
16. | ALA1220917 | Binding | Inhibition of of N-terminal N-palmitoylated Shh-mediated Gli2 transcription in human keratinocytes at 100 uM after 30 hrs by real-time quantitative PCR analysis | Homo sapiens | 1 | ALA1212710 | cell-based format | Scientific Literature | |
17. | ALA1220918 | Binding | Inhibition of of N-terminal N-palmitoylated Shh-mediated Gli1 transcription in human keratinocytes after 30 hrs by real-time quantitative PCR analysis | Homo sapiens | 1 | ALA1212710 | cell-based format | Scientific Literature | |
18. | ALA1220919 | Functional | Inhibition of of N-terminal N-palmitoylated Shh-mediated Gli1 transcription in human keratinocytes after 30 hrs by real-time quantitative PCR analysis in presence of 3.6 uM Smo agonist purmorphamine | Homo sapiens | 1 | ALA1212710 | cell-based format | Scientific Literature | |
19. | ALA1220920 | Functional | Inhibition of of N-terminal N-palmitoylated Shh-mediated Gli1 transcription in human keratinocytes after 30 hrs by real-time quantitative PCR analysis in presence of 100 nM Smo agonist SAG | Homo sapiens | 1 | ALA1212710 | cell-based format | Scientific Literature | |
20. | ALA1220921 | Functional | Inhibition of of N-terminal N-palmitoylated Shh-mediated Gli2 transcription in human keratinocytes after 30 hrs by real-time quantitative PCR analysis in presence of 3.6 uM Smo agonist purmorphamine | Homo sapiens | 1 | ALA1212710 | cell-based format | Scientific Literature |