# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1035715 | Binding | Inhibition of HO1 in rat spleen microsomal fraction assessed as carbon monoxide production | Rattus norvegicus | 3 | ALA1140121 | microsome format | Scientific Literature | |
2. | ALA1035716 | Binding | Inhibition of human recombinant HO1 expressed in Escherichia coli DH5alpha assessed as heme degradation at 25 uM by spectrophotometry in presence of L-ascorbic acid relative to control | Homo sapiens | 1 | ALA1140121 | assay format | Scientific Literature | |
3. | ALA1035717 | Binding | Inhibition of human recombinant HO1 expressed in Escherichia coli DH5alpha assessed as heme degradation at 10 uM by spectrophotometry in presence of L-ascorbic acid relative to control | Homo sapiens | 1 | ALA1140121 | assay format | Scientific Literature | |
4. | ALA1036575 | Binding | Inhibition of human recombinant HO1 expressed in Escherichia coli DH5alpha assessed as heme degradation at 25 uM by spectrophotometry in absence of L-ascorbic acid relative to control | Homo sapiens | 2 | ALA1140121 | assay format | Scientific Literature | |
5. | ALA1036576 | Binding | Inhibition of human recombinant HO1 expressed in Escherichia coli DH5alpha assessed as heme degradation at 50 uM by spectrophotometry in absence of L-ascorbic acid relative to control | Homo sapiens | 2 | ALA1140121 | assay format | Scientific Literature | |
6. | ALA4148788 | Binding | Inhibition of HO-1 in Sprague-Dawley albino rat spleen microsomes assessed as reduction in bilirubin formation using hemin as substrate after 60 mins in presence of NADPH by biliverdin reductase enzyme coupled spectrophotometric assay | Rattus norvegicus | 25 | ALA4145549 | microsome format | Scientific Literature | |
7. | ALA4148789 | Binding | Inhibition of HO-2 in Sprague-Dawley albino rat brain microsomes assessed as reduction in bilirubin formation using hemin as substrate after 60 mins in presence of NADPH by biliverdin reductase enzyme coupled spectrophotometric assay | Rattus norvegicus | 25 | ALA4145549 | microsome format | Scientific Literature | |
8. | ALA4148790 | Binding | Selectivity index, ratio of IC50 for HO-2 in Sprague-Dawley albino rat brain microsomes to IC50 for HO-1 in Sprague-Dawley albino rat spleen microsomes | Rattus norvegicus | 19 | ALA4145549 | assay format | Scientific Literature | |
9. | ALA4433390 | Binding | Inhibition of recombinant truncated HO-1 (Met1 to Pro267) in rat spleen microsomes expressed in Escherichia coli by spectrometry based assay | Rattus norvegicus | 1 | ALA4433201 | microsome format | Scientific Literature | |
10. | ALA4433391 | Binding | Inhibition of recombinant truncated HO-2 (Met1 to Ala288) in rat brain microsomes expressed in Escherichia coli by spectrometry based assay | Rattus norvegicus | 1 | ALA4433201 | microsome format | Scientific Literature |