# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1024879 | F | Inhibition of Vaccinia virus DNA synthesis in by rapid plate assay | Vaccinia virus | 93 | organism-based format | Scientific Literature | ||
2. | ALA1024880 | F | Antiviral activity against Vaccinia virus WR in BSC1 cells assessed as reduction in plaque formation after 20 hrs | Vaccinia virus | 60 | organism-based format | Scientific Literature | ||
3. | ALA1024882 | A | Cytotoxicity against african green monkey BSC1 cells after 20 hrs WST-1 based assay | Chlorocebus aethiops | 75 | cell-based format | Scientific Literature | ||
4. | ALA1837681 | B | Inhibition of His6-tagged Escherichia coli DH5alpha CDP-ME kinase assessed as ADP production after 30 mins by Kinase Glo luminescence-based assay or standard pyruvate kinase/lactate dehydrogenase-coupled absorbance-based assay | Escherichia coli DH5[alpha] | 6 | assay format | Scientific Literature | ||
5. | ALA1838042 | B | Inhibition of His6-tagged Yersinia pestis KIM6 CDP-ME kinase assessed as ADP production after 30 mins by Kinase Glo luminescence-based assay or standard pyruvate kinase/lactate dehydrogenase-coupled absorbance-based assay | Yersinia pestis | 6 | single protein format | Scientific Literature | ||
6. | ALA1838043 | F | Antimicrobial activity against Escherichia coli DH5[alpha] assessed as growth inhibition at 50 uM up to 6 hrs | Escherichia coli | 1 | organism-based format | Scientific Literature | ||
7. | ALA1838044 | F | Antimicrobial activity against Escherichia coli DH5[alpha] assessed as growth inhibition at 50 uM after overnight incubation | Escherichia coli | 1 | organism-based format | Scientific Literature | ||
8. | ALA2020225 | B | Inhibition of human galactosidase after 30 mins by Kinase-GloTM assay | Homo sapiens | 13 | single protein format | Scientific Literature | ||
9. | ALA3705442 | B | FP Assay: The FP assay was then adapted for HTS and used to screen ~120,000 small molecule library for compounds that displaced probe 5 from the T4 binding of TTR. The FP assay was performed in 384-well plate using very low concentration of probe 5 (1.5 nM) and TTR (50 nM) in a 10 μL assay volume. A detergent (0.01% Triton-X100) was added to the assay buffer to avoid any false positive hits from promiscuous, aggregate-based inhibitors. The assay demonstrated robust performance, with a very good dynamic range (−70-230 mP) and a Z′ factor in the range of 0.57-0.78 (FIGS. 4A and 4B). "Hits" were defined as compounds that resulted in at least 50% decrease in fluorescence polarization and demonstrated relative fluorescence between 70 and 130%. Many fluorescence quenchers and enhancers having less than 70% and greater than 130% total fluorescence relative to a control, respectively, were excluded from the hit list. 200 compounds were designated as positive hits (0.167% hit rate). | 33 | single protein format | BindingDB Database | |||
10. | ALA4477063 | B | Inhibition of His6-tagged recombinant human GALK expressed in Escherichia coli HMS174 (DE3) incubated for 60 mins using galactose and ATP by Kinase-Glo reagent based assay | Homo sapiens | 34 | assay format | Patent Bioactivity Data | ||
11. | ALA4477064 | B | Inhibition of Bacillus stearothermophilus glucokinase | Geobacillus stearothermophilus | 34 | assay format | Patent Bioactivity Data | ||
12. | ALA4477065 | B | Inhibition of Saccharomyces cerevisiae hexokinase | Saccharomyces cerevisiae | 34 | assay format | Patent Bioactivity Data |