#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA3436039	Functional	ST_JUDE_LEISH: Cytotoxicity against transgenic Leishmania Mexicana promastigotes LmPfHT that are glucose transport deficient and complemented with the Plasmodium falciparum hexose transporter. Activity is measured by by DNA content using SYBR green in vitro	Leishmania mexicana	791	ALA3433997	assay format	St Jude Leishmania Screening
2.	ALA3436040	Functional	ST_JUDE_LEISH: Cytotoxicity against transgenic Leishmania Mexicana promastigotes LmGT2 that are glucose transport deficient and complemented with the L. Mexicana glucose transporter 2. Activity is measured by by DNA content using SYBR green in vitro	Leishmania mexicana	791	ALA3433997	assay format	St Jude Leishmania Screening
3.	ALA3436041	Functional	ST_JUDE_LEISH: Cytotoxicity against transgenic Leishmania Mexicana promastigotes LmGLUT1 that are glucose transport deficient and complemented with the human glucose transporter GLUT1. Activity is measured by DNA content using SYBR green in vitro	Leishmania mexicana	791	ALA3433997	assay format	St Jude Leishmania Screening
4.	ALA3436042	Functional	ST_JUDE_LEISH: Cytotoxicity at 2uM final concentration against transgenic Leishmania Mexicana promastigotes LmGLUT1 that are glucose transport deficient and complemented with the human glucose transporter GLUT1. Activity is measured by DNA content using SYBR green in vitro	Leishmania mexicana	13,179	ALA3433997	assay format	St Jude Leishmania Screening
5.	ALA3436043	Functional	ST_JUDE_LEISH: Cytotoxicity at 2uM final concentration against transgenic Leishmania Mexicana promastigotes LmPfHT that are glucose transport deficient and complemented with the Plasmodium falciparum hexose transporter. Activity is measured by by DNA content using SYBR green in vitro	Leishmania mexicana	13,179	ALA3433997	assay format	St Jude Leishmania Screening
6.	ALA3436044	Functional	ST_JUDE_LEISH: Cytotoxicity at 2uM final concentration against transgenic Leishmania Mexicana promastigotes LmGT2 that are glucose transport deficient and complemented with the L. Mexicana glucose transporter 2. Activity is measured by by DNA content using SYBR green in vitro	Leishmania mexicana	13,179	ALA3433997	assay format	St Jude Leishmania Screening
7.	ALA3820729	Binding	Inhibition of Plasmodium falciparum DHODH	Plasmodium falciparum	2	ALA3817764	single protein format	Scientific Literature
8.	ALA3820730	Binding	Inhibition of human DHODH	Homo sapiens	1	ALA3817764	single protein format	Scientific Literature
9.	ALA1785545	Functional	Antimalarial activity against Plasmodium falciparum 3D7 infected in human A positive erythrocytes by [3H]hypoxanthine uptake assay in presence of serum in medium albumax	Plasmodium falciparum	3	ALA1781999	organism-based format	Scientific Literature
10.	ALA1785548	Physicochemical	Partition coefficient, log D of the compound at pH 7.4 by UV-HPLC analysis		21	ALA1781999	small-molecule physicochemical format	Scientific Literature
11.	ALA1785549	Physicochemical	Aqueous solubility of the compound in phosphate buffer at pH 6.5		21	ALA1781999	small-molecule physicochemical format	Scientific Literature
12.	ALA1785550	ADME	Plasma protein binding in human by albumin chromatographic method	Homo sapiens	24	ALA1781999	cell-free format	Scientific Literature
13.	ALA1785551	ADME	Intrinsic clearance in human liver microsome assessed per mg of protein at 1 uM after 60 mins by LC-MS analysis	Homo sapiens	21	ALA1781999	microsome format	Scientific Literature
14.	ALA1794352	Functional	PUBCHEM_BIOASSAY: qHTS profiling assay for firefly luciferase inhibitor/activator using purified enzyme and Km concentrations of substrates (counterscreen for miR-21 project). (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID2288, AID2289, AID2598, AID411]		59,860	ALA1201862	assay format	PubChem BioAssays
15.	ALA1794483	Functional	PUBCHEM_BIOASSAY: qHTS for Inhibitors of Polymerase Iota. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID588623]		114,923	ALA1201862	assay format	PubChem BioAssays
16.	ALA1794580	Functional	PUBCHEM_BIOASSAY: Primary qHTS for delayed death inhibitors of the malarial parasite plastid, 48 hour incubation. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID488752, AID488774, AID504848, AID504850]	Plasmodium falciparum	130,890	ALA1201862	organism-based format	PubChem BioAssays
17.	ALA1794584	Functional	PUBCHEM_BIOASSAY: qHTS for Inhibitors of TGF-b. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID588856, AID588860]		63,980	ALA1201862	assay format	PubChem BioAssays
18.	ALA3392923	Functional	MMV: IC50 determined against P. falciparum Dd2-luc strain using a Malaria Sybr Green I Fluorescence assay. Starting conditions were 1% trophozoite synchronised parasites at 2% haematocrit. Fluorescence detection completed after 48hr incubation at 37 degrees C under 1% O2, 3% CO2 and balance N2.	Plasmodium falciparum	315	ALA3392922	organism-based format	MMV Malaria Box
19.	ALA3392925	Toxicity	MMV: Selection for compounds that exhibit enhanced toxicity in combination with artemisinin. Assay utilized the disc diffusion method with the observance of a clear zone indicating inhibition of growth. Synthetic complete medium containing 3% glycerol as a carbon source (SCG) was used in each assay. 10 uL of each compound was added to a paper disc which was placed on SCG and SCG+Artemisinin (0.2 uM) plates seeded with 10e6 yeast cells (WC081 strain: Mat a, leu2 delta 0, lys2 delta 0, ura3 delta 0, his3 delta 1, pdr1 delta::HIS3, pdr3 delta::kanMX4, derived from BY4742, regulators of Multi-drug resistance transporters have been deleted to enhance toxicity of compounds). Plates were incubated at 30 degrees C for 5 days and clear zones were measured. Compounds that produced a larger clear zone in the presence of artemisinin was considered to have an additive interaction with artemisinin. Results are reported as diameter of clear zone on SCG+artemisinin plates - diameter of clear zone on SCG plates without artemisinin.	Saccharomyces cerevisiae	80	ALA3392924	organism-based format	MMV Malaria Box
20.	ALA3413104	Binding	Inhibition of Plasmodium falciparum DHODH using dihydroorotate substrate by DCIP assay	Plasmodium falciparum	54	ALA3407421	single protein format	Scientific Literature