#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1054501	Functional	GSK_TCMDC: Inhibition of Plasmodium falciparum Dd2 in whole red blood cells, using parasite LDH activity as an index of growth. Test compounds present at 2uM	Plasmodium falciparum	13,467	ALA1157539	organism-based format	GSK Malaria Screening
2.	ALA1054502	Functional	GSK_TCMDC: Inhibition of Plasmodium falciparum 3D7 LDH activity, using an LDH reporter assay. Test compounds present at 2uM	Plasmodium falciparum	13,467	ALA1157539	organism-based format	GSK Malaria Screening
3.	ALA1054503	Functional	GSK_TCMDC: Inhibition of Plasmodium falciparum 3D7 in whole erythrocytes, using parasite LDH activity as an index of growth.	Plasmodium falciparum	13,467	ALA1157539	organism-based format	GSK Malaria Screening
4.	ALA1054504	Functional	GSK_TCMDC: Percent inhibition of human HepG2 cell line. Test compounds present at 10uM.	Homo sapiens	13,467	ALA1157539	cell-based format	GSK Malaria Screening
5.	ALA1054505	Functional	GSK_TCMDC: Inhibition Frequency Index (IFI). The number of non-kinase HTS assays where a compound showed > 50 % inhibition, expressed as a percentage of the number of such assays in which the compound was tested		13,467	ALA1157539	assay format	GSK Malaria Screening
6.	ALA1054500	Functional	GSK_TCMDC: Inhibition of Plasmodium falciparum 3D7 in whole red blood cells, using parasite LDH activity as an index of growth. Test compounds present at 2uM	Plasmodium falciparum	13,467	ALA1157539	organism-based format	GSK Malaria Screening
7.	ALA4369188	Functional	Antitrypanosomal activity against Trypanosoma brucei brucei 427 bloodstream forms after 48 hrs by resazurin dye based fluorescence assay	Trypanosoma brucei brucei	69	ALA4368861	organism-based format	Scientific Literature
8.	ALA4369190	Functional	Antitrypanosomal activity against Trypanosoma brucei brucei 427 bloodstream forms assessed as parasite viability at 10 uM after 24 hrs by resazurin dye based fluorescence assay relative to control	Trypanosoma brucei brucei	69	ALA4368861	organism-based format	Scientific Literature
9.	ALA4369191	Functional	Antitrypanosomal activity against Trypanosoma brucei brucei 427 bloodstream forms after 24 hrs by resazurin dye based fluorescence assay	Trypanosoma brucei brucei	70	ALA4368861	organism-based format	Scientific Literature
10.	ALA4369192	ADME	Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by resazurin dye based fluorescence assay	Homo sapiens	70	ALA4368861	cell-based format	Scientific Literature
11.	ALA4369193	Toxicity	Selectivity index, ratio of IC50 for human HeLa cells to IC50 for Trypanosoma brucei brucei 427 bloodstream forms		28	ALA4368861	assay format	Scientific Literature
12.	ALA2098495	Functional	GSK_TB: Intracellular minimum inhibitory concentration (MIC) BCG assay	Mycobacterium tuberculosis variant bovis BCG	776	ALA2095176	organism-based format	GSK Tuberculosis Screening
13.	ALA2098496	Functional	GSK_TB: Intracellular minimum inhibitory concentration (MIC) H37Rv assay	Mycobacterium tuberculosis H37Rv	177	ALA2095176	organism-based format	GSK Tuberculosis Screening
14.	ALA2098497	Physicochemical	GSK_TB: GSK in-house hydrophobicity assay at pH7.4 (logD)		148	ALA2095176	assay format	GSK Tuberculosis Screening
15.	ALA2098498	Physicochemical	GSK_TB: GSK in-house hydrophobicity assay (logP)		148	ALA2095176	assay format	GSK Tuberculosis Screening
16.	ALA2098499	ADME	GSK_TB: GSK in-house kinetic solubility assay. DMSO stock solution diluted to 100 ml with pH 7.4 phosphate buffered saline.		157	ALA2095176	assay format	GSK Tuberculosis Screening
17.	ALA3431450	Functional	GSK_TCAKS: pIC50 Leishmania donovani viability assay using fluorescence intensity. Assay plates (1536-well) were prepared by adding 30nL per well of compound. For single shot assay, the final compound concentration was 5uM. For potency determinations, eleven-point one in three dilution curves were generated with a top concentration of 50uM. Briefly, eGFP LdBOB axenic amastigotes were harvested, fixed and counted in a CASY cell counter (Roche-Applied Science). A final concentration of 1.5 * 10^5 cells per well were prepared in amastigote media containing 500 U/mL penicillin/streptomycin (Invitrogen) and 6uL was dispensed to each experimental well using a Multidrop Combi dispenser (Thermo Scientific). 6uL of assay media was dispensed to control columns used as reference for 100% compound response. Following incubation for 72 h at 37C, 5% CO2, 2 uL of resazurin was added to each well at a final concentration of 0.5 mM in phosphate buffered saline (PBS) with IGEPAL (Sigma-Aldrich), 0.05% (v/v). The plates were incubated for 4 h at room temperature and resorufin fluorescence was detected using a PerkinElmer EnVision plate reader with excitation at 528 nm and emission at 590 nm.	Leishmania donovani	590	ALA3430912	organism-based format	GSK Kinetoplastid Screening
18.	ALA3431451	Functional	GSK_TCAKS: pIC50 Leishmania donovani Imaging: Infected Macrophages. Assay plates (384-well) were prepared by adding 250nL of compound to each well. For single shot assay, the final compound concentration was 5 uM. For potency determinations, eleven-point one in three dilution curves were generated with a top concentration of 50 uM. Two exposure images were taken for each well using 405 nm and 488 nm laser excitation. Automated image analysis was performed with a script developed on Acapella High Content Imaging and Analysis Software (PerkinElmer). Output: Percent of infected macrophage cells were reported for each well.	Homo sapiens	592	ALA3430912	organism-based format	GSK Kinetoplastid Screening
19.	ALA3431452	Functional	GSK_TCAKS: pIC50 Leishmania donovani Imaging: Amastigotes/Macrophages. Assay plates (384-well) were prepared by adding 250nL of compound to each well. For single shot assay, the final compound concentration was 5 uM. For potency determinations, eleven-point one in three dilution curves were generated with a top concentration of 50 uM. Two exposure images were taken for each well using 405 nm and 488 nm laser excitation. Automated image analysis was performed with a script developed on Acapella High Content Imaging and Analysis Software (PerkinElmer). Output: Average number of amastigotes per macrophage cell were reported for each well.	Homo sapiens	588	ALA3430912	organism-based format	GSK Kinetoplastid Screening
20.	ALA3431453	Functional	GSK_TCAKS: pIC50 Leishmania donovani Imaging: THP-1 Host Macrophages. Assay plates (384-well) were prepared by adding 250nL of compound to each well. For single shot assay, the final compound concentration was 5 uM. For potency determinations, eleven-point one in three dilution curves were generated with a top concentration of 50 uM. Two exposure images were taken for each well using 405 nm and 488 nm laser excitation. Automated image analysis was performed with a script developed on Acapella High Content Imaging and Analysis Software (PerkinElmer). Output: THP-1 cell count were reported for each well.	Homo sapiens	591	ALA3430912	organism-based format	GSK Kinetoplastid Screening