# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3988182 | F | GSK PKIS2: Screen against O. lienalis microfilariae, single point, at 3.1uM to assess reduction in motility. Compounds were also tested for cytotoxicity against Rhesus Monkey LLCMK2 cells. A score was assigned as follows: Score 3 = Good activity, 100 % motility reduction; Score 2 = Marginal/moderate activity, 50-99% motility reduction; Score 1 = Inactive, <50% motility reduction; Score 0 = Cytotoxic. | Onchocerca lienalis | 486 | ALA3988181 | cell-based format | Published Kinase Inhibitor Set 2 | |
2. | ALA4233136 | B | Inhibition of human full length PKMYT1 expressed in HEK293 cells using EFS (247 to 259 residues) as substrate after 1 hr by fluorescence polarization immunoasay | Homo sapiens | 18 | ALA4229416 | cell-based format | Scientific Literature | |
3. | ALA4233137 | B | Inhibition of N-[3',6'-dihydroxy-3-oxo-3H-spiro(2-benzofuran-1,9'-xanthen)-5-yl]-N'-[2-(4-{4-[N-(2-chloro-6-methylphenyl)-5-carboxamido]-thiazol-2-yl})-amino-2-methyl-pyrimid-6-yl)piperazinyl]-ethyl]thiourea binding to human PKMYT1 kinase doamin expressed in Escherichia coli BL21 after 120 mins by fluorescence polarization asay | Homo sapiens | 18 | ALA4229416 | assay format | Scientific Literature | |
4. | ALA4233138 | B | Inhibition of human full length PKMYT1 expressed in HEK293 cells assessed as decrease in GST-tagged Cdk1 phosphorylation at tyrosine 15 at 5 to 20 uM after 1 hr by Western blot method relative to control | Homo sapiens | 10 | ALA4229416 | cell-based format | Scientific Literature | |
5. | ALA4328725 | B | Displacement of (6-FAM)KI(pY)VV from PKMYT1 kinase domain (unknown origin) by fluorescence polarization binding assay | Homo sapiens | 23 | ALA4325875 | single protein format | Scientific Literature | |
6. | ALA4328727 | B | Displacement of (6-FAM)KI(pY)VV from full length PKMYT1 (unknown origin) by fluorescence polarization immuno assay | Homo sapiens | 23 | ALA4325875 | single protein format | Scientific Literature |