#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1056355	B	Inhibition of HDAC		51		assay format	Scientific Literature
2.	ALA3047468	B	Inhibition of Homo sapiens (human) HDAC8	Homo sapiens	70		single protein format	Scientific Literature
3.	ALA3706061	B	Inhibition of Histone Deacetylase Enzymatic Assay: For deacetylase assays, 20,000 cpm of the [3H]-metabolically labeled acetylated histone substrate (M. Yoshida et al., J. Biol. Chem. 265(28): 17174-17179 (1990)) is incubated with 30 ug of H446 nuclear extract or an equivalent amount of the cloned recombinant hHDAC-1 for 10 minutes at 37 C. The reaction is stopped by adding acetic acid (0.04 M, final concentration) and HCl (250 mM, final concentration). The mixture is extracted with ethyl acetate and the released [3H]-acetic acid was quantified by scintillation counting. For inhibition studies, the enzyme is preincubated with compounds at 4 C. for 30 minutes prior to initiation of the enzymatic assay. IC50 values for HDAC enzyme inhibitors are determined by performing dose response curves with individual compounds and determining the concentration of inhibitor producing fifty percent of the maximal inhibition. Alternatively, the following protocol is used to assay the compounds of the invention.	Homo sapiens	74		single protein format	BindingDB Database
4.	ALA4714468	F	Cytotoxicity against human Jurkat cells assessed as reduction in cell proliferation at IC50 measured after 48 hrs by Ki-67 staining based fluorescence assay	Homo sapiens	4		cell-based format	Scientific Literature
5.	ALA4714469	F	Induction of apoptosis in human Jurkat cells assessed as viable cells at 0.5 times IC50 measured after 48 hrs by Annexin V-FITC/7AAD staining based flow cytometry (Rvb = 85.2%)	Homo sapiens	4		cell-based format	Scientific Literature
6.	ALA4714470	F	Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	Homo sapiens	14		cell-based format	Scientific Literature
7.	ALA4714471	F	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	Homo sapiens	14		cell-based format	Scientific Literature
8.	ALA4714472	F	Cytotoxicity against human Jurkat cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	Homo sapiens	14		cell-based format	Scientific Literature
9.	ALA4714473	F	Cytotoxicity against human NAMALVA cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	Homo sapiens	14		cell-based format	Scientific Literature
10.	ALA4714474	A	Cytotoxicity against human HS5 cells assessed as reduction in cell viability measured after 72 hrs by MTT assay	Homo sapiens	14		cell-based format	Scientific Literature
11.	ALA4714475	U	Selectivity index, ratio of IC50 for human HS5 cells to IC50 for human Jurkat cells	Homo sapiens	6		assay format	Scientific Literature
12.	ALA4714476	U	Selectivity index, ratio of IC50 for human HS5 cells to IC50 for human NAMALVA cells	Homo sapiens	6		assay format	Scientific Literature
13.	ALA4714477	F	Selectivity ratio of IC50 for human HCT-116 cells to IC50 for human Jurkat cells	Homo sapiens	6		assay format	Scientific Literature
14.	ALA4714478	F	Selectivity ratio of IC50 for human HCT-116 cells to IC50 for human NAMALVA cells	Homo sapiens	6		assay format	Scientific Literature
15.	ALA4714479	F	Cytotoxicity against human Jurkat cells assessed as reduction in cell viability MTT assay	Homo sapiens	4		cell-based format	Scientific Literature
16.	ALA4714480	F	Cytotoxicity against human Jurkat cells assessed as reduction in cell viability measured after 24 hrs MTT assay	Homo sapiens	6		cell-based format	Scientific Literature
17.	ALA4714481	F	Cytotoxicity against human Jurkat cells assessed as reduction in cell viability measured after 48 hrs MTT assay	Homo sapiens	6		cell-based format	Scientific Literature
18.	ALA4714482	F	Induction of apoptosis in human Jurkat cells assessed as viable cells at IC50 measured after 48 hrs by Annexin V-FITC/7AAD staining based flow cytometry (Rvb = 85.2%)	Homo sapiens	6		cell-based format	Scientific Literature
19.	ALA4714483	F	Induction of apoptosis in human Jurkat cells assessed as early apoptotic cells at IC50 measured after 48 hrs by Annexin V-FITC/7AAD staining based flow cytometry (Rvb = 6.05%)	Homo sapiens	6		cell-based format	Scientific Literature
20.	ALA4714484	F	Induction of apoptosis in human Jurkat cells assessed as late apoptotic cells at IC50 measured after 48 hrs by Annexin V-FITC/7AAD staining based flow cytometry (Rvb = 6.77%)	Homo sapiens	6		cell-based format	Scientific Literature