PubChem BioAssay. qHTS for Agonist of gsp, the Etiologic Mutation Responsible for Fibrous Dysplasia/McCune-Albright Syndrome: qHTS. (Class of assay: confirmatory)
Inhibition of Mycobacterium tuberculosis MetAP1c expressed in Escherichia coli lacking endogenous MetAP by cell based Met-AMC substrate hydrolysis assay
Inhibition of Mycobacterium tuberculosis MetAP1c mediated cell growth of Escherichia coli lacking endogenous MetAP complemented with MtMetAP1c after 72 hrs by resazurin dye reduction method
Antibacterial activity against Burkholderia thailandensis E264 assessed as bacterial growth inhibition at 1 mM after 24 hrs by two-fold dilution method relative to DMSO-treated control
Inhibition of Burkholderia pseudomallei recombinant methionine aminopeptidase 1 using H-Met-Gly-Pro-7-amino-4-methylcoumarin hydrochloride as substrate measured for 10 mins by fluorescence assay in presence of human recombinant DPP4
Antibacterial activity against Rickettsia prowazekii str. Breinl infected with 50:1 multiplicity of infection in CD rat primary pulmonary vascular endothelial cells assessed as restoration of host cell viability by measuring fold reduction in viability at 0.3 to 300 uM measured 72 hrs post infection by WST1 assay relative to control
Inhibition of Rickettsia prowazekii str. Madrid E N-terminal His-tagged methionine aminopeptidase 1A expressed in Escherichia coli BL21(DE3) Rosetta cells using Met-AMC as substrate preincubated for 1 hr followed by substrate addition measured after 30 mins for 90 mins in presence of MnCl2 by fluorescence assay
Antibacterial activity against Rickettsia prowazekii str. Breinl infected in CD rat primary pulmonary vascular endothelial cells assessed as protection against bacterial infection by measuring restoration of host cell viability at 0.3 to 300 uM measured 72 hrs post infection by WST1 assay
Cytotoxicity against CD rat primary pulmonary vascular endothelial cells assessed as reduction in cell viability at 0.3 to 300 uM measured after 72 hrs by WST1 assay
Inhibition of Rickettsia prowazekii N-terminal His6-tagged methionine aminopeptidase 1 expressed in Escherichia coli DLB3 Rosetta cells at 10 uM using Met-AMC as substrate preincubated for 1 hr followed by 30 mins incubation after substrate addition measured after 90 mins in presence of MnCl2 by fluorescence assay relative to control