# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1068578 | B | Inhibition of pleckstrin homology domain of AKT by surface plasmon resonance spectroscopy | 14 | ALA1152590 | single protein format | Scientific Literature | ||
2. | ALA4345047 | B | Displacement of 2FAMInsP5 from recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells by fluorescence polarization assay | Homo sapiens | 6 | ALA4342482 | cell-based format | Scientific Literature | |
3. | ALA4345048 | B | Binding affinity to recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as increase in release of phosphate at 100 uM incubated for 10 mins by phosphate release assay | Homo sapiens | 6 | ALA4342482 | cell-based format | Scientific Literature | |
4. | ALA4345052 | B | Inhibition of recombinant human brain INPP5A expressed in Escherichia coli incubated for 10 mins by malachite green reagent based phosphate assay | Homo sapiens | 6 | ALA4342482 | assay format | Scientific Literature | |
5. | ALA4345059 | B | Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 3-OH-Bz(1,2,4)P3 product formation at 100 uM incubated for 2 hrs in presence of AS1949490 by fluorescence based HPLC analysis | Homo sapiens | 1 | ALA4342482 | cell-based format | Scientific Literature | |
6. | ALA4345060 | B | Substrate activity at recombinant human N-terminal His-tagged SHIP2 (419 to 832 residues) expressed in Escherichia coli Rosetta2 (DE3) cells assessed as 1,2-diOH-Bz(3,4)P2 product formation at 100 uM incubated for 2 hrs in presence of AS1949490 by fluorescence based HPLC analysis | Homo sapiens | 2 | ALA4342482 | cell-based format | Scientific Literature |