# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1070011 | Binding | Inhibition of SHP2 Src homology-2 domain expressed in Escherichia coli BL21 (DE3) assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry | 9 | ALA1157613 | assay format | Scientific Literature | ||
2. | ALA1070012 | Binding | Inhibition of SHP2 Src homology-2 domain expressed in Escherichia coli BL21 (DE3) assessed as inhibition of p-nitrophenyl phosphate hydrolysis by Lineweaver-Burke plot analysis | 1 | ALA1157613 | assay format | Scientific Literature | ||
3. | ALA1070013 | Binding | Inhibition of SHP1 expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry | 1 | ALA1157613 | assay format | Scientific Literature | ||
4. | ALA1070014 | Binding | Inhibition of PTP1B expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry | 1 | ALA1157613 | assay format | Scientific Literature | ||
5. | ALA1070015 | Binding | Inhibition of HePTP expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry | 1 | ALA1157613 | assay format | Scientific Literature | ||
6. | ALA1070016 | Binding | Inhibition of Lyp expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry | 1 | ALA1157613 | assay format | Scientific Literature | ||
7. | ALA1070017 | Binding | Inhibition of FAP1 expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry | 1 | ALA1157613 | assay format | Scientific Literature | ||
8. | ALA1070018 | Binding | Inhibition of CD45 expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry | 1 | ALA1157613 | assay format | Scientific Literature | ||
9. | ALA1070019 | Binding | Inhibition of LAR expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry | 1 | ALA1157613 | assay format | Scientific Literature | ||
10. | ALA1070020 | Binding | Inhibition of PTPalpha expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry | 1 | ALA1157613 | assay format | Scientific Literature | ||
11. | ALA1070021 | Binding | Inhibition of VHR expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry | 1 | ALA1157613 | assay format | Scientific Literature | ||
12. | ALA1070022 | Binding | Inhibition of Cdc14A expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry | 1 | ALA1157613 | assay format | Scientific Literature | ||
13. | ALA1070023 | Binding | Inhibition of LMWPTP expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry | 1 | ALA1157613 | assay format | Scientific Literature | ||
14. | ALA1070024 | Binding | Inhibition of SHP2-dependent ERK1/2 activation in EGF-stimulated HEK293 cells assessed as phosphorylated ERK1/2 level at 10 uM treated for 3 hrs before EGF challenge measured after 60 mins of EGF stimulation by immunoblot analysis | Homo sapiens | 1 | ALA1157613 | cell-based format | Scientific Literature | |
15. | ALA1070025 | Binding | Inhibition of SHP2-dependent ERK1/2 activation in EGF-stimulated HEK293 cells assessed as phosphorylated ERK1/2 level at 10 uM treated for 3 hrs before EGF challenge measured after 5 to 15 mins of EGF stimulation by immunoblot analysis | Homo sapiens | 1 | ALA1157613 | cell-based format | Scientific Literature | |
16. | ALA1070026 | Binding | Inhibition of SHP2-dependent ERK1/2 activation in EGF-stimulated mouse NIH/3T3 cells assessed as phosphorylated ERK1/2 level at 10 uM treated for 3 hrs before EGF challenge measured after 60 mins of EGF stimulation by immunoblot analysis | Mus musculus | 1 | ALA1157613 | cell-based format | Scientific Literature | |
17. | ALA1070027 | Binding | Inhibition of SHP2-dependent ERK1/2 activation in EGF-stimulated mouse NIH/3T3 cells assessed as phosphorylated ERK1/2 level at 10 uM treated for 3 hrs before EGF challenge measured after 5 to 15 mins of EGF stimulation by immunoblot analysis | Mus musculus | 1 | ALA1157613 | cell-based format | Scientific Literature | |
18. | ALA1070028 | Binding | Inhibition of SHP2-dependent ERK1/2 activation in EGF-stimulated HEK293 cells assessed as phosphorylated ERK1/2 level treated for 3 hrs before EGF challenge measured after 60 mins of EGF stimulation by immunoblot analysis | Homo sapiens | 1 | ALA1157613 | cell-based format | Scientific Literature | |
19. | ALA1073447 | Functional | Antiproliferative activity against GM-CSF-stimulated C57/BL6 mouse low density BM-MNC expressing wild type SHP2 assessed as inhibition of hyperproliferation at 10 uM after 16 hrs by [3H]thymidine incorporation assay | Mus musculus | 1 | ALA1157613 | assay format | Scientific Literature | |
20. | ALA1073448 | Functional | Antiproliferative activity against GM-CSF-stimulated C57/BL6 mouse low density BM-MNC expressing SHP2 E76K mutant assessed as inhibition of hyperproliferation at 10 uM after 16 hrs by [3H]thymidine incorporation assay | Mus musculus | 1 | ALA1157613 | assay format | Scientific Literature |