# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1113303 | B | Inhibition of 3C-like protease of SARS coronavirus assessed as concentration of FRET peptide for 60 mins | SARS coronavirus | 6 | ALA1155394 | assay format | Scientific Literature | |
2. | ALA1113304 | B | Inhibition of 3C-like protease of SARS coronavirus assessed as concentration of FRET peptide for 60 mins by dixon plot | SARS coronavirus | 6 | ALA1155394 | assay format | Scientific Literature | |
3. | ALA3039488 | A | Inhibition of sodium fluorescein uptake in OATP1B1-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 uM | Cricetulus griseus | 1,862 | ALA3039007 | cell-based format | Scientific Literature | |
4. | ALA3039491 | A | Inhibition of sodium fluorescein uptake in OATP1B3-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 uM | Cricetulus griseus | 1,878 | ALA3039007 | cell-based format | Scientific Literature | |
5. | ALA4133422 | F | Antiproliferative activity against human A549 cells after 72 hrs by MTT assay | Homo sapiens | 21 | ALA4130422 | cell-based format | Scientific Literature | |
6. | ALA4133423 | F | Antiproliferative activity against human MIAPaCa2 cells after 72 hrs by MTT assay | Homo sapiens | 21 | ALA4130422 | cell-based format | Scientific Literature | |
7. | ALA4192289 | F | Antiproliferative activity against human A549 cells after 72 hrs by MTT assay | Homo sapiens | 23 | ALA4190255 | cell-based format | Scientific Literature | |
8. | ALA4192290 | F | Antiproliferative activity against human MIAPaCa2 cells after 72 hrs by MTT assay | Homo sapiens | 23 | ALA4190255 | cell-based format | Scientific Literature | |
9. | ALA4372442 | B | Inhibition of human N-terminal His6-tagged PTP1B catalytic domain (1 to 393 residues) expressed in Escherichia coli strain Rosetta2 (DE3) at 500 uM using DiFMUP as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by fluorescence assay | Homo sapiens | 2 | ALA4371044 | assay format | Scientific Literature | |
10. | ALA4372443 | B | Inhibition of human N-terminal His6-tagged TCPTP catalytic domain (1 to 336 residues) expressed in Escherichia coli strain Rosetta2 (DE3) at 500 uM using DiFMUP as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by fluorescence assay | Homo sapiens | 2 | ALA4371044 | assay format | Scientific Literature | |
11. | ALA4392938 | B | Inhibition of Escherichia coli GroEL expressed in Escherichia coliDH5alpha/Escherichia coli GroES expressed in Escherichia coli BL21 (DE3) assessed as reduction in GroEL/GroES-mediated denatured soluble pig heart MDH refolding by measuring MDH enzyme activity using sodium mesoxalate as substrate after 20 to 40 mins by malachite green dye based spectrometric analysis relative to untreated control | Escherichia coli | 150 | ALA4392822 | assay format | Scientific Literature | |
12. | ALA4392939 | B | Inhibition of Escherichia coli GroEL expressed in Escherichia coli DH5alpha/Escherichia coli GroES expressed in Escherichia coli BL21 (DE3) assessed as reduction in GroEL/GroES-mediated denatured rhodanese refolding by measuring rhodanese enzyme activity after 45 mins by Fe(SCN)3 dye based spectrometric analysis relative to untreated control | Escherichia coli | 150 | ALA4392822 | assay format | Scientific Literature | |
13. | ALA4396409 | A | Induction of apoptosis in TNFalpha-treated human HepG2 cells assessed as normal cells at 2 uM incubated for 10 hrs by FITC Annexin V and propidium iodide staining based flow cytometric analysis (Rvb = 88.37%) | Homo sapiens | 3 | ALA4393721 | cell-based format | Scientific Literature | |
14. | ALA4396410 | B | Binding affinity to GFP-Nur77 (unknown origin) expressed in human HepG2 cells cotransfected with mCherry-p62/SQSTM1 assessed as co-localization with p62/SQSTM1 in cytoplasm in presence of TNFalpha by DAPI staining based confocal microscopic analysis | Homo sapiens | 3 | ALA4393721 | cell-based format | Scientific Literature | |
15. | ALA4396411 | B | Binding affinity to recombinant human N-terminal His-tagged Nur77 LBD (367 to 598 residues) expressed in Escherichia coli BL21(DE3) incubated for 30 secs by fluorescence quenching assay | Homo sapiens | 25 | ALA4393721 | assay format | Scientific Literature | |
16. | ALA4396413 | F | Antiinflammatory activity in human HepG2 cells assessed as inhibition of TNFalpha-induced IkappaBalpha degradation at 2 uM pretreated for 1 hr followed by TNFalpha addition and measured after 30 mins by Western blot analysis | Homo sapiens | 25 | ALA4393721 | cell-based format | Scientific Literature | |
17. | ALA4396414 | B | Binding affinity to Myc-Nur77 (unknown origin) expressed in human HepG2 cells co-transfected with Flag tagged TRAF2 assessed as effect on protein interaction with TRAF2 at 2 uM incubated for 1 hr in presence of TNFalpha by co-immunoprecipitation assay | Homo sapiens | 3 | ALA4393721 | cell-based format | Scientific Literature | |
18. | ALA4396415 | A | Induction of apoptosis in TNFalpha-treated human HepG2 cells assessed as PARP cleavage at 2 uM incubated for 10 hrs by Western blot analysis | Homo sapiens | 3 | ALA4393721 | cell-based format | Scientific Literature | |
19. | ALA4396416 | A | Induction of apoptosis in TNFalpha-treated human HepG2 cells assessed as non-viable apoptotic cells at 2 uM incubated for 10 hrs by FITC Annexin V and propidium iodide staining based flow cytometric analysis (Rvb = 6.65%) | Homo sapiens | 3 | ALA4393721 | cell-based format | Scientific Literature | |
20. | ALA4396417 | A | Induction of apoptosis in TNFalpha-treated human HepG2 cells assessed as viable apoptotic cells at 2 uM incubated for 10 hrs by FITC Annexin V and propidium iodide staining based flow cytometric analysis (Rvb = 3.69%) | Homo sapiens | 3 | ALA4393721 | cell-based format | Scientific Literature |