# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1115314 | Binding | Inhibition of GST-fused human recombinant SET7 at 100 uM after 90 mins by SDS-PAGE based scintillation counting | Homo sapiens | 6 | ALA1156943 | single protein format | Scientific Literature | |
2. | ALA1115316 | Binding | Inhibition of GST-fused human recombinant PRMT1 at 100 uM after 90 mins by SDS-PAGE based scintillation counting | Homo sapiens | 3 | ALA1156943 | single protein format | Scientific Literature | |
3. | ALA5136927 | Binding | Binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS at 100 uM incubated for 5 mins by microscale thermophoresis assay | Homo sapiens | 38 | ALA5131574 | assay format | Scientific Literature | |
4. | ALA5136928 | Binding | Inhibition of full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS at 100 uM using tRNA asp as substrate and SAM as co-substrate incubated for 5 mins by tritium incorporation assay relative to control | Homo sapiens | 38 | ALA5131574 | assay format | Scientific Literature | |
5. | ALA5136929 | Binding | Binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS assessed as dissociation constant at 100 uM by isothermal titration calorimetry assay | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
6. | ALA5136930 | Binding | Inhibition of full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS by isothermal titration calorimetry assay | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
7. | ALA5136931 | Binding | Selectivity ratio of Kd for binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS assessed as dissociation constant to IC50 for inhibition of full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS | 4 | ALA5131574 | assay format | Scientific Literature | ||
8. | ALA5136932 | Binding | Binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS assessed as gibbs free energy change at 100 uM by isothermal titration calorimetry assay | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
9. | ALA5136933 | Binding | Binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS assessed as change in enthalpy change at 100 uM by isothermal titration calorimetry assay | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
10. | ALA5136934 | Binding | Binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS assessed as change in entropy at 100 uM by isothermal titration calorimetry assay | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
11. | ALA5136935 | Binding | Inhibition of recombinant human DNMT3A-3L expressed in Escherichia coli Rosetta2(DE3)pLysS at 100 uM using Poly(dI-dC)-Poly(dI-dC) as substrate measured at 150 mins by liquid scintillation counting analysis | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
12. | ALA5136936 | Binding | Inhibition of full length recombinant human NSUN2 expressed in Escherichia coli Rosetta2(DE3)pLysS at 100 uM measured at 20 mins by liquid scintillation counting analysis | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
13. | ALA5136937 | Binding | Inhibition of full length human NSUN6 expressed in Escherichia coli Rosetta2(DE3)pLysS at 100 uM measured at 20 mins by liquid scintillation counting analysis | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
14. | ALA5136938 | Binding | Inhibition of recombinant human EHMT2 G9a expressed in Escherichia coli Rosetta2(DE3)pLysS at 100 uM using histone H3 as substrate measured at 120 mins by liquid scintillation counting analysis | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
15. | ALA5136939 | Binding | Inhibition of full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS at 100 uM by liquid scintillation counting analysis | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature |