#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA2051182	B	Competitive inhibition of [3H]m7-GTP binding to human FLAG-His6 tagged eIF4E expressed in Escherichia coli by scintillation proximity assay	Homo sapiens	26		assay format	Scientific Literature
2.	ALA2064994	B	Inhibition of Ascaris suum eIF4E-3 assessed as inhibition of cap-dependent translation after 1 hr by cell-free Renilla luciferase reporter gene assay	Ascaris suum	16		assay format	Scientific Literature
3.	ALA1023603	B	Binding affinity to mouse eukaryotic initiation factor 4E expressed in Escherichia coli BL21(DE3) by fluorescence quenching titration method	Mus musculus	6		assay format	Scientific Literature
4.	ALA1023604	A	Stability in presence of human DcpS assessed as complete degradation at 40 uM by HPLC-based assay		6		assay format	Scientific Literature
5.	ALA1023605	B	Inhibition of eukaryotic initiation factor 4E in micrococcal nuclease-pretreated rabbit reticulocyte lysate assessed as inhibition of m7,3'-O-GpppG-capped 5'UTRbetaglob-LUC mRNA translation by luciferase-based luminometry	Oryctolagus cuniculus	6		assay format	Scientific Literature
6.	ALA1023606	B	Inhibition of eukaryotic initiation factor 4E in micrococcal nuclease-pretreated rabbit reticulocyte lysate assessed as inhibition of m7,3'-O-GpppG-capped 5'UTRbetaglob-LUC mRNA translation treated 60 mins before mRNA addition by luciferase-based luminometry	Oryctolagus cuniculus	6		assay format	Scientific Literature
7.	ALA1023607	B	Inhibition of eukaryotic initiation factor 4E in micrococcal nuclease-pretreated rabbit reticulocyte lysate assessed as inhibition of rabbit beta-globulin mRNA translation by [3H]leucine incorporation assay	Oryctolagus cuniculus	4		assay format	Scientific Literature
8.	ALA1117203	B	Binding affinity to mouse eIF4E expressed in Escherichia coli by fluorescence time-synchronized titration method in presence of KKRYDREFLLGFQFIFA	Mus musculus	19		assay format	Scientific Literature
9.	ALA1117204	B	Binding affinity to mouse eIF4E expressed in Escherichia coli assessed as fluorescence quenching by time-synchronized fluorescence titration assay in presence of KKRYDREFLLGFQFIFA	Mus musculus	19		assay format	Scientific Literature
10.	ALA1117205	B	Binding affinity to mouse eIF4E expressed in Escherichia coli by fluorescence time-synchronized titration method in absence of KKRYDREFLLGFQFIFA	Mus musculus	19		assay format	Scientific Literature
11.	ALA1117206	B	Binding affinity to mouse eIF4E expressed in Escherichia coli assessed as fluorescence quenching by time-synchronized fluorescence titration assay in absence of KKRYDREFLLGFQFIFA	Mus musculus	19		assay format	Scientific Literature
12.	ALA3608038	B	Binding affinity to eIF4E (unknown origin) by fluorescence quenching assay	Homo sapiens	11		single protein format	Scientific Literature
13.	ALA3608039	A	Stability of the compound assessed as human DcpS-mediated compound hydrolysis at 40 uM after 10 to 180 mins by HPLC analysis	Homo sapiens	11		single protein format	Scientific Literature
14.	ALA3608041	A	Stability of the compound in 5% fetal bovine serum at 50 uM up to 240 mins by RP-HPLC analysis	Bos taurus	5		cell-free format	Scientific Literature
15.	ALA3608042	A	Stability of the compound in 10% fetal bovine serum at 50 uM up to 240 mins by RP-HPLC analysis	Bos taurus	5		cell-free format	Scientific Literature
16.	ALA3608043	B	Inhibition of eIF4E-mediated translation in flexi rabbit reticulocyte lysate using luciferase mRNA treated simultaneously with mRNA by luminescence based luciferase assay	Oryctolagus cuniculus	11		assay format	Scientific Literature
17.	ALA3608168	B	Inhibition of eIF4E-mediated translation in flexi rabbit reticulocyte lysate using luciferase mRNA preincubated for 60 mins before mRNA addition by luminescence based luciferase assay	Oryctolagus cuniculus	11		assay format	Scientific Literature