# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1117203 | B | Binding affinity to mouse eIF4E expressed in Escherichia coli by fluorescence time-synchronized titration method in presence of KKRYDREFLLGFQFIFA | Mus musculus | 19 | ALA1157016 | assay format | Scientific Literature | |
2. | ALA1117204 | B | Binding affinity to mouse eIF4E expressed in Escherichia coli assessed as fluorescence quenching by time-synchronized fluorescence titration assay in presence of KKRYDREFLLGFQFIFA | Mus musculus | 19 | ALA1157016 | assay format | Scientific Literature | |
3. | ALA1117205 | B | Binding affinity to mouse eIF4E expressed in Escherichia coli by fluorescence time-synchronized titration method in absence of KKRYDREFLLGFQFIFA | Mus musculus | 19 | ALA1157016 | assay format | Scientific Literature | |
4. | ALA1117206 | B | Binding affinity to mouse eIF4E expressed in Escherichia coli assessed as fluorescence quenching by time-synchronized fluorescence titration assay in absence of KKRYDREFLLGFQFIFA | Mus musculus | 19 | ALA1157016 | assay format | Scientific Literature | |
5. | ALA4182516 | B | Inhibition of human DCPS using m7GMPF as substrate after 30 mins by nucleoside 5'-fluorophosphate probe based HTS assay | Homo sapiens | 6 | ALA4177717 | single protein format | Scientific Literature | |
6. | ALA4623582 | B | Binding affinity to murine eIF4E assessed as intrinsic fluorescence quenching of tryptophan residues by fluorescence spectrophotometric method | Mus musculus | 10 | ALA4622862 | single protein format | Scientific Literature | |
7. | ALA4623583 | B | Displacement of pyrene-labeled 7-methylguanosine-pentaphosphate probe from murine eIF4E assessed as decrease in fluorescence intensity incubated for 15 mins by fluorescence assay | Mus musculus | 6 | ALA4622862 | single protein format | Scientific Literature | |
8. | ALA4623584 | B | Inhibition of eIF4E in rabbit reticulocyte lysate system assessed as reduction in ARCA mRNA cap-dependent translation measured after 60 mins by luciferase reporter gene based luminescence assay | Oryctolagus cuniculus | 6 | ALA4622862 | assay format | Scientific Literature | |
9. | ALA4623588 | B | Inhibition of human DcpS using pyrene labeled 7-methylguanosine triphosphate as substrate preincubated for 15 mins followed by enzyme addition by fluorescence assay | Homo sapiens | 10 | ALA4622862 | single protein format | Scientific Literature |