# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1117203 | B | Binding affinity to mouse eIF4E expressed in Escherichia coli by fluorescence time-synchronized titration method in presence of KKRYDREFLLGFQFIFA | Mus musculus | 19 | ALA1157016 | assay format | Scientific Literature | |
2. | ALA1117204 | B | Binding affinity to mouse eIF4E expressed in Escherichia coli assessed as fluorescence quenching by time-synchronized fluorescence titration assay in presence of KKRYDREFLLGFQFIFA | Mus musculus | 19 | ALA1157016 | assay format | Scientific Literature | |
3. | ALA1117205 | B | Binding affinity to mouse eIF4E expressed in Escherichia coli by fluorescence time-synchronized titration method in absence of KKRYDREFLLGFQFIFA | Mus musculus | 19 | ALA1157016 | assay format | Scientific Literature | |
4. | ALA1117206 | B | Binding affinity to mouse eIF4E expressed in Escherichia coli assessed as fluorescence quenching by time-synchronized fluorescence titration assay in absence of KKRYDREFLLGFQFIFA | Mus musculus | 19 | ALA1157016 | assay format | Scientific Literature | |
5. | ALA2051182 | B | Competitive inhibition of [3H]m7-GTP binding to human FLAG-His6 tagged eIF4E expressed in Escherichia coli by scintillation proximity assay | Homo sapiens | 26 | ALA2046428 | assay format | Scientific Literature | |
6. | ALA2051184 | B | Inhibition of eIF4E in rabbit reticulocyte cell lysate assessed as inhibition of cap-dependent translation after 90 mins by luciferase reporter gene assay | Oryctolagus cuniculus | 11 | ALA2046428 | assay format | Scientific Literature | |
7. | ALA2064994 | B | Inhibition of Ascaris suum eIF4E-3 assessed as inhibition of cap-dependent translation after 1 hr by cell-free Renilla luciferase reporter gene assay | Ascaris suum | 16 | ALA2062407 | assay format | Scientific Literature | |
8. | ALA4182512 | B | Substrate activity at recombinant His6/SUMO-tagged human cN3B (1 to 300 residues) expressed in Escherichia coli BL21(DE3) RIL assessed as compound dephosphorylation at 100 uM up to 150 mins by HPLC method | Homo sapiens | 5 | ALA4177717 | assay format | Scientific Literature | |
9. | ALA4182513 | B | Substrate activity at recombinant His6/SUMO-tagged human cN3B (1 to 300 residues) expressed in Escherichia coli BL21(DE3) RIL assessed as compound dephosphorylation up to 30 mins by malachite green dye based assay | Homo sapiens | 2 | ALA4177717 | assay format | Scientific Literature | |
10. | ALA4182514 | B | Substrate activity at recombinant His6/SUMO-tagged human cN3B (1 to 300 residues) expressed in Escherichia coli BL21(DE3) RIL assessed as compound dephosphorylation by measuring ratio of Kcat to Km up to 30 mins by malachite green dye based assay | Homo sapiens | 2 | ALA4177717 | assay format | Scientific Literature | |
11. | ALA4182515 | B | Inhibition of fluorescein-labeled m7GDPC4H5 binding to mouse eIF4E (28 to 217 residues) by MST method | Mus musculus | 5 | ALA4177717 | single protein format | Scientific Literature | |
12. | ALA4182516 | B | Inhibition of human DCPS using m7GMPF as substrate after 30 mins by nucleoside 5'-fluorophosphate probe based HTS assay | Homo sapiens | 6 | ALA4177717 | single protein format | Scientific Literature | |
13. | ALA4182521 | B | Substrate activity at recombinant His6/SUMO-tagged human cN3B (1 to 300 residues) expressed in Escherichia coli BL21(DE3) RIL assessed as compound dephosphorylation by Vmax up to 30 mins by malachite green dye based assay | Homo sapiens | 2 | ALA4177717 | assay format | Scientific Literature | |
14. | ALA4182522 | B | Substrate activity at recombinant His6/SUMO-tagged human cN3B (1 to 300 residues) expressed in Escherichia coli BL21(DE3) RIL assessed as compound dephosphorylation by Kcat up to 30 mins by malachite green dye based assay | Homo sapiens | 2 | ALA4177717 | assay format | Scientific Literature | |
15. | ALA4623582 | B | Binding affinity to murine eIF4E assessed as intrinsic fluorescence quenching of tryptophan residues by fluorescence spectrophotometric method | Mus musculus | 10 | ALA4622862 | single protein format | Scientific Literature | |
16. | ALA4623584 | B | Inhibition of eIF4E in rabbit reticulocyte lysate system assessed as reduction in ARCA mRNA cap-dependent translation measured after 60 mins by luciferase reporter gene based luminescence assay | Oryctolagus cuniculus | 6 | ALA4622862 | assay format | Scientific Literature | |
17. | ALA4623588 | B | Inhibition of human DcpS using pyrene labeled 7-methylguanosine triphosphate as substrate preincubated for 15 mins followed by enzyme addition by fluorescence assay | Homo sapiens | 10 | ALA4622862 | single protein format | Scientific Literature |