# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1117203 | B | Binding affinity to mouse eIF4E expressed in Escherichia coli by fluorescence time-synchronized titration method in presence of KKRYDREFLLGFQFIFA | Mus musculus | 19 | ALA1157016 | assay format | Scientific Literature | |
2. | ALA1117204 | B | Binding affinity to mouse eIF4E expressed in Escherichia coli assessed as fluorescence quenching by time-synchronized fluorescence titration assay in presence of KKRYDREFLLGFQFIFA | Mus musculus | 19 | ALA1157016 | assay format | Scientific Literature | |
3. | ALA1117205 | B | Binding affinity to mouse eIF4E expressed in Escherichia coli by fluorescence time-synchronized titration method in absence of KKRYDREFLLGFQFIFA | Mus musculus | 19 | ALA1157016 | assay format | Scientific Literature | |
4. | ALA1117206 | B | Binding affinity to mouse eIF4E expressed in Escherichia coli assessed as fluorescence quenching by time-synchronized fluorescence titration assay in absence of KKRYDREFLLGFQFIFA | Mus musculus | 19 | ALA1157016 | assay format | Scientific Literature | |
5. | ALA2051182 | B | Competitive inhibition of [3H]m7-GTP binding to human FLAG-His6 tagged eIF4E expressed in Escherichia coli by scintillation proximity assay | Homo sapiens | 26 | ALA2046428 | assay format | Scientific Literature | |
6. | ALA2051184 | B | Inhibition of eIF4E in rabbit reticulocyte cell lysate assessed as inhibition of cap-dependent translation after 90 mins by luciferase reporter gene assay | Oryctolagus cuniculus | 11 | ALA2046428 | assay format | Scientific Literature | |
7. | ALA4623582 | B | Binding affinity to murine eIF4E assessed as intrinsic fluorescence quenching of tryptophan residues by fluorescence spectrophotometric method | Mus musculus | 10 | ALA4622862 | single protein format | Scientific Literature | |
8. | ALA4623584 | B | Inhibition of eIF4E in rabbit reticulocyte lysate system assessed as reduction in ARCA mRNA cap-dependent translation measured after 60 mins by luciferase reporter gene based luminescence assay | Oryctolagus cuniculus | 6 | ALA4622862 | assay format | Scientific Literature |