#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA1217267	B	Inhibition of rat recombinant NAAA expressed in HEK293 cells after 30 mins	Rattus norvegicus	25		cell-based format	Scientific Literature
2.	ALA2421060	B	Inhibition of recombinant rat lung NAAA enzyme using heptadecenoylethanolamide as substrate by LC/MS analysis	Rattus norvegicus	2		single protein format	Scientific Literature
3.	ALA3888344	B	NAAA Assay: Recombinant NAAA or native rat lung NAAA was incubated at 37 °C. for 30 min in 0.2 ml of sodium hydrogen phosphate buffer (50 mM, pH 5.0) containing 0.1% Triton X-100, 3 mM dithiothreitol (DTT) and 50 mM heptadecenoylethanolamide as substrate. The reaction was terminated by the addition of 0.2 ml cold methanol containing 1 mmol of heptadecanoic acid (HDA, NuChek Prep, Elysian, Minn.). Samples were analyzed by LC/MS (liquid chromatography/mass spectrometry). Heptadecanoic acid was eluted on an XDB Eclipse C18 column isocratically at 2.2 ml/min for 1 min with a solvent mixture of 95% methanol and 5% water, both containing 0.25% acetic acid and 5 mM ammonium acetate. The column temperature was 50 °C. ESI was in the negative mode, capillary voltage was 4 kV, and fragmentor voltage was 100 V. N2 was used as drying gas at a flow rate of 13 liters/min and a temperature of 350 °C. Nebulizer pressure was set at 60 psi.		25		single protein format	BindingDB Database
4.	ALA4612015	B	Inhibition of rat NAAA	Rattus norvegicus	4		single protein format	Scientific Literature