# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3595950 | Binding | Displacement of [3H]-MK-801 from rat cortical membranes NMDA receptor by beta counting analysis in absence of spermine | Rattus norvegicus | 81 | ALA3593210 | assay format | Scientific Literature | |
2. | ALA3595951 | Binding | Displacement of [3H]-MK-801 from rat cortical membranes NMDA receptor by beta counting analysis in presence of 30 uM spermine | Rattus norvegicus | 79 | ALA3593210 | assay format | Scientific Literature | |
3. | ALA3595952 | Binding | Ratio of IC50 for displacement of [3H]-MK-801 from rat cortical membranes NMDA receptor in presence of 30 uM spermine to IC50 for displacement of [3H]-MK-801 from rat cortical membranes NMDA receptor in absence of 30 spermine | Rattus norvegicus | 79 | ALA3593210 | assay format | Scientific Literature | |
4. | ALA3595953 | Binding | Displacement of [3H]-ifenprodil from rat cortical membranes NMDA receptor by beta counting analysis in absence of spermine | Rattus norvegicus | 79 | ALA3593210 | assay format | Scientific Literature | |
5. | ALA4814081 | Functional | Cytotoxicity against mouse MigA9 cells harboring mouse Hoxa9 gene incubated for 72 hrs by MTS assay | Mus musculus | 10 | ALA4813909 | cell-based format | Scientific Literature | |
6. | ALA4814082 | ADME | Drug localization in nucleus of human HT-29 cells at 5 uM incubated for 16 hrs by DiOC6/propidium iodide staining based by fluorescence microscopy | Homo sapiens | 7 | ALA4813909 | subcellular format | Scientific Literature | |
7. | ALA4814083 | Binding | Inhibition of HOXA9 (unknown origin) expressed in reticulocyte lysate system binding to DNA Hoxa9-binding site (5'-biotin-cctctcctctcctGCGATGATTTACGACCGC) (unknown origin) assessed as Hoxa9/DNA remaining complexes at 5 to 10 uM incubated for 20 mins with DNA followed by compound wash out and subsequent HOXA9 addition and measured after 1 hr by ELISA | 39 | ALA4813909 | subcellular format | Scientific Literature | ||
8. | ALA4814084 | Binding | Binding affinity to DNA Hoxa9-binding site (strand a-CTGCGATGATTTA/strand b-GCGGTCGTAAATCATCGCAG) (unknown origin) at 0.5 uM by spectrophotometric method | 4 | ALA4813909 | nucleic acid format | Scientific Literature | ||
9. | ALA4814085 | Binding | Inhibition of HOXA9 (unknown origin) expressed in reticulocyte lysate system binding to DNA Hoxa9-binding site (strand a-CTGCGATGATTTACGACCGC/strand b-GCGGTCGTAAATCATCGCAG) (unknown origin) assessed as HOXA9/DNA inhibition at 2.5 uM incubated for 20 mins by electrophoretic mobility shift assay relative to control | 5 | ALA4813909 | subcellular format | Scientific Literature | ||
10. | ALA4814089 | Binding | Inhibition of HMG-D100 (unknown origin) expressed in reticulocyte lysate system binding to 32P-radiolabeled 117 bp1 DNA fragment (unknown origin) assessed as HMG-D100/DNA inhibition at 2.5 to 5 uM incubated for 20 mins by electrophoretic mobility shift assay relative to control | 2 | ALA4813909 | subcellular format | Scientific Literature | ||
11. | ALA4814090 | Binding | Binding affinity to DNA Hoxa9-binding site (strand a-CTGCGATGATTTA/strand b-GCGGTCGTAAATCATCGCAG) (unknown origin) at 1 uM by spectrophotometric method | 5 | ALA4813909 | nucleic acid format | Scientific Literature | ||
12. | ALA4814092 | Binding | Binding affinity to DNA Hoxa9-binding site (strand a-CTGCGATGATTTACGACCGC/strand b-GCGGTCGTAAATCATCGCAG) (unknown origin) assessed as compound-DNA complex formation at 1 to 10 uM at wavelength of 300 to 400 nM by circular dichroism method | Not specified | 9 | ALA4813909 | nucleic acid format | Scientific Literature | |
13. | ALA4814093 | Binding | Binding affinity to DNA Hoxa9-binding site (strand a-CTGCGATGATTTACGACCGC/strand b-GCGGTCGTAAATCATCGCAG) (unknown origin) assessed as change in positive ellipticity band of the DNA helix at 1 to 10 uM at wavelength of 300 to 400 nM by circular dichroism method | Not specified | 9 | ALA4813909 | nucleic acid format | Scientific Literature | |
14. | ALA4814094 | Binding | Binding affinity to DNA FP-Hoxa9 binding site-1R (strand a-cgcgGTTCCTCTGATCGCGGACCACCGACAGCTGCGATGATTTACGACCGCGCTCGAACCTGAACCTGATCTCAAGTGCT/strand b-ctagAGCACTTGAGATCAGGTTCAGGTTCGAGCGCGGTCGTAAATCATCGCAGCTGTCGGTGGTCCGCGATCAGAGGAAC) (unknown origin) assessed as recognition of HOXA9 binding site 5'-ATTTA at 0.1 to 1 uM by DNase1footprinting based densitometric analysis | Not specified | 4 | ALA4813909 | nucleic acid format | Scientific Literature | |
15. | ALA4814096 | Binding | Binding affinity to DNA Hoxa9 binding site-5'ATGA mutant (strand a-CTGCGTAGATTTACGACCGC/strand b-GCGGTCGTAAATCTACGCAG) (unknown origin) by UV/visible spectrophotometric method | Not specified | 3 | ALA4813909 | nucleic acid format | Scientific Literature | |
16. | ALA4814097 | Binding | Binding affinity to DNA Hoxa9 binding site-5'-ATTTA mutant (strand a-CTGCGATGAGTCACGACCGC/strand b-GCGGTCGTGACTCATCGCAG) (unknown origin) assessed as decrease in deltaTm by UV/visible spectrophotometric method relative to wild type HBS | Not specified | 2 | ALA4813909 | nucleic acid format | Scientific Literature | |
17. | ALA4814098 | Binding | Binding affinity to DNA Hoxa9-binding site-5'-ATTTA/5'ATGA double mutant (strand a-CTGCGTAGCGTCACGACCGC/strand b-GCGGTCGTGACGCTACGCAG) (unknown origin) assessed as decrease in deltaTm by UV/visible spectrophotometric method relative to wild type HBS | Not specified | 2 | ALA4813909 | nucleic acid format | Scientific Literature | |
18. | ALA4814101 | Binding | Binding affinity to DNA FP-Hoxa9-binding site-1R mutant (strand a-cgcgGTTCCTCTGATCGCGGACCACCGACAGCTGCGATGCTGTCCGACCGCGCTCGAACCTGAACCTGATCTCAAGTGCT/strand b-ctagAGCACTTGAGATCAGGTTCAGGTTCGAGCGCGGTCGGACAGCATCGCAGCTGTCGGTGGTCCGCGATCAGAGGAAC) (unknown origin) at 0.5 to 1.5 uM by DNase1footprinting based densitometric analysis | Not specified | 2 | ALA4813909 | nucleic acid format | Scientific Literature | |
19. | ALA4814102 | Binding | Binding affinity to DNA Hoxa9-binding site ATGATTTA (unknown origin) by surface plasmon resonance assay | Not specified | 2 | ALA4813909 | nucleic acid format | Scientific Literature | |
20. | ALA4814103 | Binding | Binding affinity to hairpin DNA Hoxa9-binding site 5'-biotin-CCATGATTTACCTCTGTAAATCATGG (unknown origin) by surface plasmon resonance | Not specified | 2 | ALA4813909 | nucleic acid format | Scientific Literature |