# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1259735 | P | Lipophilicity, log P of the compound | 52 | small-molecule physicochemical format | Scientific Literature | |||
2. | ALA1259736 | B | Inhibition of human recombinant soluble epoxide hydrolase by fluorescence assay | Homo sapiens | 52 | single protein format | Scientific Literature | ||
3. | ALA1259737 | F | Antiinflammatory activity in Sprague-Dawley rat assessed as reduction in carrageenan-induced inflammatory pain at 10 uL, intraplanter | Rattus norvegicus | 53 | organism-based format | Scientific Literature | ||
4. | ALA1259738 | B | Inhibition of mouse recombinant soluble epoxide hydrolase by fluorescence assay | Mus musculus | 52 | single protein format | Scientific Literature | ||
5. | ALA3705305 | B | Inhibition Assay: Inhibition assay of human soluble epoxide hydrolases. | Homo sapiens | 74 | single protein format | BindingDB Database | ||
6. | ALA3888169 | B | Fluorescent Based Assay: IC50 values were determined using a sensitive fluorescent based assay (Anal. Biochem. 2005, 343, 66-75). Cyano(2-methoxynaphthalen-6-yl)methyl trans-(3-phenyl-oxyran-2-yl)methyl carbonate (CMNPC) was used as the fluorescent substrate. Human sEH (1 nM) or murine sEH (1 nM) was incubated with the inhibitor for 5 min in pH 7.0 Bis-Tris/HCl buffer (25 mM) containing 0.1 mg/mL of BSA at 30° C. prior to substrate introduction ([S]=5 μM). Activity was determined by monitoring the appearance of 6-methoxy-2-naphthaldehyde over 10 minutes by fluorescence detection with an excitation wavelength of 330 nm and an emission wavelength of 465 nm. | 52 | single protein format | BindingDB Database | |||
7. | ALA3888170 | B | Fluorescent Based Assay: IC50 values were determined using a sensitive fluorescent based assay (Anal. Biochem. 2005, 343, 66-75). Cyano(2-methoxynaphthalen-6-yl)methyl trans-(3-phenyl-oxyran-2-yl)methyl carbonate (CMNPC) was used as the fluorescent substrate. Human sEH (1 nM) or murine sEH (1 nM) was incubated with the inhibitor for 5 min in pH 7.0 Bis-Tris/HCl buffer (25 mM) containing 0.1 mg/mL of BSA at 30° C. prior to substrate introduction ([S]=5 μM). Activity was determined by monitoring the appearance of 6-methoxy-2-naphthaldehyde over 10 minutes by fluorescence detection with an excitation wavelength of 330 nm and an emission wavelength of 465 nm. | 51 | single protein format | BindingDB Database |