# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA1291058 | B | Inhibition of human neuraminidase 3 assessed as inhibition of 4-methylumbelliferyl-alpha-D-glucopyranoside hydrolysis by fluorescence assay | 21 | ALA1287720 | single protein format | Scientific Literature | ||
2. | ALA2390084 | B | Ratio of DANA IC50 to compound IC50 for human NEU4 using GM3 as substrate | Homo sapiens | 1 | ALA2384918 | single protein format | Scientific Literature | |
3. | ALA2390086 | B | Inhibition of human NEU4 using GM3 as substrate preincubated for 30 mins before substrate addition measured after 1 hr by fluorescence plate reader analysis | Homo sapiens | 2 | ALA2384918 | single protein format | Scientific Literature | |
4. | ALA2390087 | B | Inhibition of human NEU4 using 4-MU-NANA as substrate measured every 30 seconds for 60 mins by fluorescence plate reader analysis | Homo sapiens | 2 | ALA2384918 | single protein format | Scientific Literature | |
5. | ALA2390088 | B | Selectivity ratio of IC50 for human NEU3 to IC50 for human NEU4 | Homo sapiens | 1 | ALA2384918 | assay format | Scientific Literature | |
6. | ALA2390089 | B | Inhibition of human NEU3 using 4-MU-NANA as substrate preincubated for 15 mins before substrate addition measured after 30 mins by fluorescence plate reader analysis | Homo sapiens | 11 | ALA2384918 | single protein format | Scientific Literature | |
7. | ALA2390090 | B | Inhibition of human NEU4 using 4-MU-NANA as substrate preincubated for 15 mins before substrate addition measured after 30 mins by fluorescence plate reader analysis | Homo sapiens | 11 | ALA2384918 | single protein format | Scientific Literature | |
8. | ALA2390091 | B | Inhibition of human NEU2 using 4-MU-NANA as substrate preincubated for 15 mins before substrate addition measured after 30 mins by fluorescence plate reader analysis | Homo sapiens | 11 | ALA2384918 | single protein format | Scientific Literature | |
9. | ALA2390092 | B | Inhibition of human NEU1 using 4-MU-NANA as substrate preincubated for 15 mins before substrate addition measured after 30 mins by fluorescence plate reader analysis | Homo sapiens | 11 | ALA2384918 | single protein format | Scientific Literature | |
10. | ALA4418863 | B | Inhibition of His6-tagged human neuraminidase-1 expressed in HEK293 cells pre-incubated for 15 mins before 4MU-NANA substrate addition and measured after 30 mins by fluorescence based assay | Homo sapiens | 71 | ALA4418500 | cell-based format | Patent Bioactivity Data | |
11. | ALA4418864 | B | Inhibition of N-terminal MBP tagged human neuraminidase-2 expressed in Escherichia coli pre-incubated for 15 mins before 4MU-NANA substrate addition and measured after 30 mins by fluorescence based assay | Homo sapiens | 67 | ALA4418500 | assay format | Patent Bioactivity Data | |
12. | ALA4418865 | B | Inhibition of N-terminal MBP tagged human neuraminidase-3 expressed in Escherichia coli pre-incubated for 15 mins before 4MU-NANA substrate addition and measured after 30 mins by fluorescence based assay | Homo sapiens | 67 | ALA4418500 | assay format | Patent Bioactivity Data | |
13. | ALA4418866 | B | Inhibition of N-terminal GST tagged human neuraminidase-4 expressed in Escherichia coli pre-incubated for 15 mins before 4MU-NANA substrate addition and measured after 30 mins by fluorescence based assay | Homo sapiens | 67 | ALA4418500 | assay format | Patent Bioactivity Data | |
14. | ALA4418872 | B | Selectivity index, ratio of IC50 for inhibition of His6-tagged human neuraminidase-1 expressed in HEK293 cells to IC50 for inhibition of N-terminal GST tagged human neuraminidase-4 expressed in Escherichia coli | Homo sapiens | 18 | ALA4418500 | cell-based format | Patent Bioactivity Data | |
15. | ALA4418873 | B | Selectivity index, ratio of IC50 for inhibition of N-terminal MBP tagged human neuraminidase-2 expressed in Escherichia coli to IC50 for inhibition of N-terminal GST tagged human neuraminidase-4 expressed in Escherichia coli | Homo sapiens | 18 | ALA4418500 | assay format | Patent Bioactivity Data | |
16. | ALA4418874 | B | Selectivity index, ratio of IC50 for inhibition of N-terminal MBP tagged human neuraminidase-3 expressed in Escherichia coli to IC50 for inhibition of N-terminal GST tagged human neuraminidase-4 expressed in Escherichia coli | Homo sapiens | 4 | ALA4418500 | assay format | Patent Bioactivity Data | |
17. | ALA4418879 | B | Inhibition of His6-tagged human neuraminidase-1 expressed in HEK293 cells pre-incubated for 15 mins before 4MU-NANA substrate addition and measured every 30 secs for 30 mins by fluorescence based assay | Homo sapiens | 5 | ALA4418500 | cell-based format | Patent Bioactivity Data | |
18. | ALA4418880 | B | Inhibition of N-terminal MBP tagged human neuraminidase-2 expressed in Escherichia coli pre-incubated for 15 mins before 4MU-NANA substrate addition and measured every 30 secs for 30 mins by fluorescence based assay | Homo sapiens | 8 | ALA4418500 | assay format | Patent Bioactivity Data | |
19. | ALA4418881 | B | Inhibition of N-terminal MBP tagged human neuraminidase-3 expressed in Escherichia coli pre-incubated for 15 mins before 4MU-NANA substrate addition and measured every 30 secs for 30 mins by fluorescence based assay | Homo sapiens | 8 | ALA4418500 | assay format | Patent Bioactivity Data | |
20. | ALA4418882 | B | Inhibition of N-terminal GST tagged human neuraminidase-4 expressed in Escherichia coli pre-incubated for 15 mins before 4MU-NANA substrate addition and measured every 30 secs for 30 mins by fluorescence based assay | Homo sapiens | 8 | ALA4418500 | assay format | Patent Bioactivity Data |