PUBCHEM_BIOASSAY: qHTS profiling assay for firefly luciferase inhibitor/activator using purified enzyme and Km concentrations of substrates (counterscreen for miR-21 project). (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID2288, AID2289, AID2598, AID411]
PUBCHEM_BIOASSAY: qHTS Assay for Inhibitors of the Phosphatase Activity of Eya2. (Class of assay: confirmatory) [Related pubchem assays (depositor defined):AID488939]
PubChem BioAssay. Small Molecule Inhibitors of FGF22-Mediated Excitatory Synaptogenesis & Epilepsy Measured in Biochemical System Using RT-PCR - 7012-01_Inhibitor_Dose_CherryPick_Activity. (Class of assay: confirmatory)
Inhibition of sclerostin binding to LRP6 (unknown origin) expressed in rat MC3T3-E1 cells co-expressing TCF/LEF luciferase plasmid assessed as reduction in sclerostin-induced inhibition of Wnt3a activation by measuring luciferase activity at 3 uM after 24 hrs by dual-luciferase reporter gene assay (Rvb = 100 +/- 3.1%)
Inhibition of sclerostin binding to human ALP-fused LRP5 at 50 uM using alkaline phosphatase as substrate pretreated for 2 hrs followed by substrate addition measured after 25 mins by ELISA relative to control
Inhibition of sclerostin binding to LRP6 (unknown origin) expressed in rat MC3T3-E1 cells co-expressing TCF/LEF luciferase plasmid assessed as reduction in sclerostin-induced inhibition of Wnt3a activation by measuring luciferase activity at 0.3 uM after 24 hrs by dual-luciferase reporter gene assay (Rvb = 100 +/- 2.6%)